##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088226_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3269579
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.966837014796095	31.0	30.0	33.0	27.0	34.0
2	30.800709510306984	31.0	31.0	34.0	27.0	34.0
3	30.790525324514256	31.0	31.0	34.0	27.0	34.0
4	34.97279496840419	35.0	35.0	37.0	32.0	37.0
5	34.883924199415276	35.0	35.0	37.0	32.0	37.0
6	34.98897197467931	35.0	35.0	37.0	32.0	37.0
7	34.598791159351094	35.0	35.0	37.0	30.0	37.0
8	34.82589103979442	35.0	35.0	37.0	32.0	37.0
9	36.22526049989922	38.0	35.0	39.0	32.0	39.0
10	36.17004238160326	38.0	35.0	39.0	32.0	39.0
11	36.266014982357056	38.0	35.0	39.0	32.0	39.0
12	35.99867322367803	38.0	35.0	39.0	30.0	39.0
13	36.167665011305736	38.0	35.0	39.0	32.0	39.0
14	37.06823906074758	39.0	36.0	40.0	31.0	41.0
15	37.11227164108896	39.0	36.0	40.0	31.0	41.0
16	37.02838836437352	39.0	36.0	40.0	31.0	41.0
17	37.13609886777472	39.0	36.0	40.0	31.0	41.0
18	37.13593370889647	39.0	36.0	40.0	31.0	41.0
19	37.217067396138766	39.0	36.0	40.0	31.0	41.0
20	37.178131496440365	39.0	36.0	40.0	31.0	41.0
21	37.10663666484278	39.0	36.0	40.0	31.0	41.0
22	37.030438475412275	39.0	36.0	40.0	31.0	41.0
23	36.89756846370741	39.0	35.0	40.0	31.0	41.0
24	36.827796178040046	39.0	35.0	40.0	31.0	41.0
25	36.73891286921038	38.0	35.0	40.0	30.0	41.0
26	36.47515475233968	38.0	35.0	40.0	30.0	41.0
27	36.33276608395148	38.0	35.0	40.0	30.0	41.0
28	36.16305340840518	38.0	35.0	40.0	30.0	41.0
29	35.997841006441504	38.0	34.0	40.0	29.0	41.0
30	35.83551766144816	38.0	34.0	40.0	29.0	41.0
31	35.57561783948331	38.0	34.0	40.0	28.0	41.0
32	35.33478652756211	38.0	34.0	40.0	27.0	41.0
33	35.460638510340324	38.0	34.0	40.0	27.0	41.0
34	35.45400248778206	38.0	34.0	40.0	27.0	41.0
35	35.356874386580046	38.0	34.0	40.0	27.0	41.0
36	35.26952950211633	38.0	34.0	40.0	26.0	41.0
37	35.10539827910566	38.0	33.0	40.0	26.0	41.0
38	34.97751728892313	38.0	33.0	40.0	25.0	41.0
39	34.92904529910426	38.0	33.0	40.0	25.0	41.0
40	34.76133685713054	38.0	33.0	40.0	24.0	41.0
41	34.59417772135189	38.0	33.0	40.0	24.0	41.0
42	34.37607747052449	38.0	33.0	40.0	23.0	41.0
43	34.03248399870442	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	11.0
16	49.0
17	99.0
18	308.0
19	637.0
20	1348.0
21	2793.0
22	5008.0
23	8445.0
24	14049.0
25	22113.0
26	33363.0
27	47781.0
28	67181.0
29	89511.0
30	114446.0
31	140196.0
32	170397.0
33	206325.0
34	248014.0
35	295238.0
36	358802.0
37	448566.0
38	517771.0
39	477120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.54866972169812	25.044753468259977	13.391877058177826	22.01469975186408
2	18.107835901808762	22.795381301384673	35.889299509202864	23.207483287603694
3	20.165012070361353	26.42450297117763	32.02069746594287	21.38978749251815
4	13.099362333805056	15.662719879226042	35.010348427121656	36.227569359847244
5	11.275580128206109	40.586265081834696	34.61500700854758	13.523147781411613
6	32.19405311815374	35.40697441474881	15.157823071410723	17.241149395686723
7	24.610263278544426	35.266008253662015	21.1395412069872	18.984187260806358
8	28.09107839266156	33.336035006341795	19.950458453519552	18.62242814747709
9	25.154308857501224	13.471887359198234	23.712043660667014	37.661760122633524
10	16.48362067409902	27.659279680961983	33.59502859542467	22.262071049514326
11	31.579080976480455	21.28047678309654	25.099959352564966	22.04048288785804
12	20.32656192127488	27.09409988258427	31.683283994667207	20.896054201473646
13	31.437044341182762	20.44009335758518	28.934489730940893	19.18837257029116
14	21.113176956421604	22.26595534165102	28.20207127584316	28.418796426084214
15	23.35517814373043	28.475409219352095	26.93132663257257	21.238086004344904
16	22.10489485037676	28.009844692542984	27.154444043101574	22.73081641397868
17	19.529242144019154	26.647253361977185	27.77449329103227	26.04901120297139
18	20.793013412430163	25.917312290053246	32.41977025176636	20.869904045750232
19	19.910055698302443	24.277590478774176	33.678189149122865	22.13416467380051
20	24.686664552225228	23.234826257447825	32.39120388282406	19.687305307502893
21	24.067532853618157	24.12215762335151	31.084124286337783	20.72618523669255
22	22.20099284953812	23.713328229720094	32.100554842075994	21.985124078665784
23	21.20936671051533	25.95120044507259	33.26367094968496	19.57576189472712
24	19.86399472225629	24.87947225009703	31.387955452368637	23.868577575278042
25	20.95242231492189	26.029712082197737	32.66527586579189	20.352589737088476
26	22.501582007958824	24.851150560974364	31.232797861743055	21.414469569323757
27	21.169086295208036	26.442578692853118	30.796900763064606	21.591434248874243
28	20.940402418782355	25.81274225213705	30.925143573530416	22.32171175555018
29	19.974467660821162	25.789191819497248	31.88266746269168	22.353673056989905
30	19.17109205802949	26.138105242295723	34.00667180698188	20.684130892692913
31	20.82032579729684	25.36075745531764	32.791530652723175	21.02738609466234
32	21.875721614311814	25.65935859020382	30.438505997255305	22.02641379822907
33	22.876829096345432	25.61256357469876	30.012426676339675	21.49818065261613
34	21.019525755456588	28.694734092676764	30.017014422957818	20.26872572890883
35	19.55814494771345	29.941805963397734	30.09797285827931	20.402076230609506
36	20.78714109675894	25.844703553576775	32.534249822377745	20.83390552728654
37	20.875287001782187	26.389941946654293	31.381471437148328	21.35329961441519
38	21.091094602699613	25.864583788922058	32.06743131149301	20.976890296885315
39	22.23745014266363	25.198993509561934	31.87401191407212	20.68954443370232
40	20.274200439873148	26.266225712851714	30.837884632853342	22.621689214421796
41	21.59984511767417	25.68832256385302	30.66997310662932	22.04185921184348
42	18.94097068766346	27.621323723941217	31.10158830846418	22.33611727993115
43	19.626257692504144	27.418637078351676	30.569409700759635	22.38569552838454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	352.0
2	476.0
3	1292.5
4	2109.0
5	2109.0
6	3844.0
7	5579.0
8	6063.5
9	6548.0
10	10253.0
11	13958.0
12	13958.0
13	26378.0
14	38798.0
15	61918.5
16	85039.0
17	81837.0
18	78635.0
19	78635.0
20	84130.0
21	89625.0
22	71442.0
23	53259.0
24	58373.0
25	63487.0
26	63487.0
27	68902.0
28	74317.0
29	78227.0
30	82137.0
31	88421.5
32	94706.0
33	94706.0
34	102813.5
35	110921.0
36	120979.0
37	131037.0
38	137352.0
39	143667.0
40	143667.0
41	152932.5
42	162198.0
43	187131.0
44	212064.0
45	270503.0
46	328942.0
47	328942.0
48	336580.5
49	344219.0
50	315475.0
51	286731.0
52	268842.0
53	250953.0
54	250953.0
55	217235.5
56	183518.0
57	149836.0
58	116154.0
59	102160.5
60	88167.0
61	88167.0
62	78134.0
63	68101.0
64	59173.0
65	50245.0
66	43382.5
67	36520.0
68	36520.0
69	31007.5
70	25495.0
71	21355.0
72	17215.0
73	13951.5
74	10688.0
75	10688.0
76	8394.5
77	6101.0
78	4772.0
79	3443.0
80	2726.0
81	2009.0
82	2009.0
83	1509.5
84	1010.0
85	805.5
86	601.0
87	484.0
88	367.0
89	367.0
90	296.0
91	225.0
92	139.0
93	53.0
94	35.5
95	18.0
96	18.0
97	15.5
98	13.0
99	8.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3269579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.62014289347916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40687751905045	27.577438133628938
2	14.34934027395279	11.083471436144245
3	5.652271182687214	6.548745622442239
4	2.761684266989774	4.266265640712733
5	1.5421300320636788	2.977864109931244
6	0.9463564395124475	2.192905255328094
7	0.6329795320320218	1.7112031983006981
8	0.4252528799219668	1.3138661590759917
9	0.32245755090666384	1.1208021023787023
>10	1.671479605616978	12.158356890064878
>50	0.16591567272368501	4.49369009755705
>100	0.10651265995652331	7.602148544431861
>500	0.007930603224838729	2.042677906250136
>1k	0.007289746398538484	6.019010047109527
>5k	8.010710328064268E-4	2.385898284451035
>10k+	7.209639295257842E-4	6.505656572192706
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42626	1.3037152489663042	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37930	1.1600881948409871	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34043	1.0412043874761858	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	24585	0.7519316707135689	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15565	0.47605517407592846	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14808	0.4529023461430356	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13971	0.42730272001379993	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13967	0.42718038010398285	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	12789	0.3911512766628364	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9783	0.2992128344352591	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9597	0.2935240286287623	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9316	0.2849296499641085	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8930	0.27312384866675493	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7906	0.24180483175356826	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7831	0.23951095844449696	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7580	0.23183412910347173	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5671	0.17344740714324383	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5478	0.16754450649456704	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5028	0.1537812666401393	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4998	0.15286371731651077	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4967	0.15191558301542799	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4943	0.15118154355652516	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4924	0.15060042898489379	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4292	0.13127072323378638	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	4035	0.1234103840280354	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4020	0.12295160936622115	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3838	0.11738514346954149	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3660	0.11194101748267897	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3578	0.1094330493314277	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3576	0.10937187937651913	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3447	0.1054264172849165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.058497745428387E-5	0.0	0.0	0.0	0.0
3	3.058497745428387E-5	0.0	0.0	0.0	0.0
4	3.058497745428387E-5	0.0	0.0	0.0	0.0
5	3.058497745428387E-5	0.0	0.0	0.0	0.0
6	3.058497745428387E-5	0.0	0.0	0.0	0.0
7	3.058497745428387E-5	0.0	0.0	0.0	0.0
8	3.058497745428387E-5	0.0	0.0	0.0	0.0
9	3.058497745428387E-5	0.0	0.0	3.058497745428387E-5	0.0
10	3.058497745428387E-5	0.0	0.0	6.116995490856774E-5	0.0
11	3.058497745428387E-5	0.0	0.0	3.058497745428387E-4	0.0
12	3.058497745428387E-5	0.0	0.0	5.199446167228257E-4	0.0
13	3.058497745428387E-5	0.0	0.0	6.116995490856774E-4	0.0
14	3.058497745428387E-5	0.0	0.0	9.175493236285161E-4	0.0
15	3.058497745428387E-5	0.0	0.0	0.001406908962897058	0.0
16	3.058497745428387E-5	0.0	0.0	0.0022327033541627225	0.0
17	3.058497745428387E-5	0.0	0.0	0.0033337625425169416	0.0
18	3.058497745428387E-5	0.0	0.0	0.0038231221817854838	0.0
19	3.058497745428387E-5	0.0	0.0	0.0046489165730511485	0.0
20	3.058497745428387E-5	0.0	0.0	0.0056888058064968	0.0
21	3.058497745428387E-5	0.0	0.0	0.008472038754836632	0.0
22	3.058497745428387E-5	0.0	0.0	0.013732654876973458	0.0
23	3.058497745428387E-5	0.0	0.0	0.022969318068167186	0.0
24	3.058497745428387E-5	0.0	0.0	0.03731367249422632	0.0
25	3.058497745428387E-5	0.0	0.0	0.0434306679850831	0.0
26	3.058497745428387E-5	0.0	0.0	0.0510157423937455	0.0
27	6.116995490856774E-5	0.0	0.0	0.10056340586968536	0.0
28	1.2233990981713548E-4	0.0	0.0	0.15390360654995644	0.0
29	1.2233990981713548E-4	0.0	0.0	0.21797913431668114	0.0
30	1.2233990981713548E-4	0.0	0.0	0.3428270122850679	0.0
31	1.5292488727141934E-4	0.0	0.0	0.5096374793207321	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9820	0.0	29.181772	1
GTATTGG	1380	0.0	21.985508	1
ATTGGAC	1495	0.0	21.408028	3
CGCGATA	70	1.22007084E-4	18.5	14
TTGGACC	2330	0.0	18.023605	4
TATTGGA	1775	0.0	17.926762	2
GTATCAA	16935	0.0	17.565989	2
TGGACCC	2345	0.0	17.434969	5
ACGTTTA	225	0.0	17.266666	26
GGACCCT	2370	0.0	17.251055	6
CGTCTGT	950	0.0	15.968421	34
CGATCTG	330	0.0	15.69697	33
GACCCTC	2570	0.0	15.40467	7
CGTATAC	170	1.4897523E-9	15.235294	3
GAACCGT	135	3.9783663E-7	15.074075	6
TAAACGT	175	2.242814E-9	14.799999	4
ATCACGT	240	0.0	14.645833	23
AGTATCA	710	0.0	14.591549	1
TGCGACG	140	6.00412E-7	14.535714	22
CTTGCGA	115	2.2121998E-5	14.478261	10
>>END_MODULE