FastQCFastQC Report
Wed 25 May 2016
SRR2088225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347726
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT417121.7766979621983143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT375971.6014219717292393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT318461.3564615291562985No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG195680.8334873831102948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138370.589378828704883No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT134300.5720429044956694No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA125500.534559825124397No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT114930.4895375354704936No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107230.45673984102063014No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA92610.3944668159742662No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT84990.36200987679141433No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG84960.361882093566285No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT82670.3521279740480789No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA68620.2922828302791723No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA66630.28380654301225955No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65770.2801434238918851No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT58200.24789945675091554No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.21765742680363892No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48240.2054754260079754No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC48240.2054754260079754No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG44270.18856544588252633No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43940.1871598304061036No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42450.18081326355801317No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC37510.15977162582004883No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36600.15589553465779227No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36580.15581034584103937No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA32450.13821885518156718No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC31610.1346409248779457No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31490.13412979197742836No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC30850.13140374984133582No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG29510.12569609911889207No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC29300.12480161654298672No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA29020.12360897310844622No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA28910.1231404346163053No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC28360.12079774215560078No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG27370.11658089572633262No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG26960.11483452498289835No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA26810.11419560885725165No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC26640.11347150391485207No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC26140.11134178349602977No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA26000.11074546177875953No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA25850.11010654565311284No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC25010.10652861534949137No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT24970.10635823771598561No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA24560.1046118669725513No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG24250.10329144031288148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89500.029.682681
GTATTGG7900.025.291141
ATTGGAC8800.024.8068183
TATTGGA9500.022.0052622
ATCACGT1101.7571438E-920.18181823
TTGGACC17050.019.5307924
CGTCTGT8700.019.35057634
GGACCCT17300.018.7138756
TGGACCC17800.018.3960695
AGTATCA4900.018.1224481
ACGTTTA1258.591087E-917.7626
GTATCAA156500.017.6134192
ATAACGC752.0684228E-417.2666663
TCACGTT1301.3951649E-817.07692324
TTTTTAC19750.016.9544321
CGATCTG2650.016.75471733
GACCCTC19400.016.4020617
GCGTCTG10200.016.3235333
GCGCGTG1151.2434684E-616.08695633
AACGGTA700.002593572715.85714318
Produced by FastQC (version 0.11.5)