##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088225_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2347726
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.823571830784342	31.0	30.0	33.0	27.0	34.0
2	30.67698147058047	31.0	31.0	34.0	27.0	34.0
3	30.669286790707265	31.0	30.0	34.0	27.0	34.0
4	34.868658437994895	35.0	35.0	37.0	32.0	37.0
5	34.79345928783853	35.0	35.0	37.0	32.0	37.0
6	34.89955727371934	35.0	35.0	37.0	32.0	37.0
7	34.50331767846844	35.0	35.0	37.0	30.0	37.0
8	34.749015004306294	35.0	35.0	37.0	31.0	37.0
9	36.08613398667477	38.0	35.0	39.0	31.0	39.0
10	36.04278608321414	37.0	35.0	39.0	31.0	39.0
11	36.15301146726662	38.0	35.0	39.0	31.0	39.0
12	35.883253837969164	37.0	35.0	39.0	30.0	39.0
13	36.05395263331411	37.0	35.0	39.0	31.0	39.0
14	36.88828934892743	38.0	36.0	40.0	31.0	41.0
15	36.93526587003765	38.0	36.0	40.0	31.0	41.0
16	36.83805052207966	38.0	36.0	40.0	31.0	41.0
17	37.003987688512204	38.0	36.0	40.0	31.0	41.0
18	37.02415614087845	38.0	36.0	40.0	31.0	41.0
19	37.12778237324117	39.0	36.0	40.0	31.0	41.0
20	37.09432148385289	39.0	36.0	40.0	31.0	41.0
21	37.03449806323225	39.0	36.0	40.0	31.0	41.0
22	36.96851336143996	39.0	36.0	40.0	31.0	41.0
23	36.82465330281302	39.0	35.0	40.0	31.0	41.0
24	36.748650822114676	38.0	35.0	40.0	30.0	41.0
25	36.666381426111904	38.0	35.0	40.0	30.0	41.0
26	36.37882614921843	38.0	35.0	40.0	30.0	41.0
27	36.22119318864297	38.0	35.0	40.0	30.0	41.0
28	36.02779412929788	38.0	34.0	40.0	30.0	41.0
29	35.848025706577346	38.0	34.0	40.0	29.0	41.0
30	35.65161266689554	38.0	34.0	40.0	29.0	41.0
31	35.34575542461088	37.0	34.0	40.0	27.0	41.0
32	35.099756530361724	37.0	33.0	40.0	27.0	41.0
33	35.15668054960417	37.0	33.0	40.0	27.0	41.0
34	35.1025694650909	37.0	33.0	40.0	27.0	41.0
35	34.97698922276279	37.0	33.0	40.0	26.0	41.0
36	34.85921440576967	37.0	33.0	40.0	25.0	41.0
37	34.66851199841889	37.0	33.0	40.0	25.0	41.0
38	34.507894873592576	37.0	33.0	40.0	24.0	41.0
39	34.39815378796333	37.0	33.0	40.0	23.0	41.0
40	34.17514565157944	37.0	33.0	40.0	22.0	41.0
41	33.95612775937226	37.0	33.0	40.0	21.0	41.0
42	33.68882356799729	37.0	33.0	40.0	19.0	41.0
43	33.31646239808223	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	2.0
15	19.0
16	43.0
17	84.0
18	209.0
19	516.0
20	1165.0
21	2316.0
22	4330.0
23	6946.0
24	11424.0
25	18041.0
26	26972.0
27	38505.0
28	53258.0
29	70162.0
30	88995.0
31	106940.0
32	128721.0
33	154968.0
34	183415.0
35	215043.0
36	259700.0
37	322697.0
38	351468.0
39	301784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.96855936340101	26.063646268772423	14.392735779217848	20.575058588608723
2	18.020331163006244	23.523017592342548	36.019322527415895	22.437328717235317
3	20.44374002758414	26.455472231427347	33.034306388394555	20.066481352593957
4	12.84498276204293	15.940914740476531	34.38591215499594	36.8281903424846
5	10.45952551532845	41.75448923767083	34.221071794579096	13.56491345242162
6	31.49209064430858	35.2285147414988	14.89811843460438	18.381276179588248
7	23.28772608047106	37.06654865175919	20.656371314199358	18.98935395357039
8	28.49122938537121	33.27969277505126	19.81440764382215	18.41467019575538
9	24.71497951634901	13.61078763024305	25.159366978940472	36.51486587446746
10	16.72767605759786	28.653343703651956	32.97650577622772	21.642474462522458
11	30.188616559172576	21.555624463842886	25.825671309173217	22.43008766781132
12	20.238264601576166	28.31552745081837	31.73308980690251	19.713118140702964
13	31.738967835258457	20.788201008124457	30.140910821790957	17.331920334826126
14	20.763198090407485	23.274607002691113	29.473967575432567	26.488227331468835
15	22.92469393787861	28.976550074412433	28.22944415148957	19.86931183621939
16	21.23386630296721	28.390365826335785	28.402973771215212	21.972794099481796
17	18.313252909411066	27.272518172904338	28.58284995778894	25.831378959895662
18	19.618302987656993	26.071654017547193	34.457981893968885	19.85206110082693
19	18.48886965514715	24.173945341151395	36.56261420625746	20.774570797443996
20	24.36613131174592	22.7145757213576	34.759039172373605	18.16025379452287
21	23.46031862321242	23.763846377302976	33.22282923986871	19.553005759615903
22	21.013908778111244	23.64070594268667	34.26724413325916	21.078141145942926
23	20.0810486402587	26.45692044131215	35.47011022581	17.991920692619157
24	18.193179272197863	25.043680565790044	33.49585939756173	23.267280764450366
25	19.697102643153418	26.33872095806751	35.13131430158374	18.83286209719533
26	21.513370810733452	24.83739584602292	33.25456207410916	20.394671269134474
27	19.974307052867328	26.77735817552815	32.69495673685941	20.55337803474511
28	19.609400756306314	26.28262412223573	32.763704111978996	21.34427100947896
29	18.43183574233109	26.096018019138516	33.94966022440438	21.52248601412601
30	17.503873961441837	26.314016201209174	36.86967729624326	19.312432541105736
31	19.450310641020288	25.566313956569036	34.94918061136606	20.03419479104461
32	21.018423785399147	26.138612427514968	32.165550835148565	20.67741295193732
33	22.24829473286065	26.113779887431498	31.43173436763915	20.2061910120687
34	19.88979974664846	29.750788635471093	31.58856697928123	18.770844638599225
35	17.91111909992904	31.124202739161216	31.72853220520623	19.236145955703517
36	19.374492594110215	26.247696707367048	34.787534831577446	19.59027586694529
37	19.280742301273655	26.932146255568156	33.440103316996954	20.34700812616123
38	19.958632310584797	26.367557372538364	33.886492716782115	19.78731760009473
39	21.519461811131283	25.554131955773375	33.5413076312994	19.38509860179595
40	19.081017120396503	26.884909056678673	32.289969102016165	21.74410472090866
41	20.465846525531514	26.22831625155576	32.1061742298718	21.199662993040928
42	17.227010306994938	28.388491672367216	32.668335231624134	21.71616278901371
43	18.072977851759532	28.21321568189814	32.15733011433191	21.556476352010414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	281.5
2	355.0
3	1060.0
4	1765.0
5	1765.0
6	3225.5
7	4686.0
8	5346.0
9	6006.0
10	9414.5
11	12823.0
12	12823.0
13	24064.0
14	35305.0
15	58887.5
16	82470.0
17	78484.0
18	74498.0
19	74498.0
20	80058.0
21	85618.0
22	66333.0
23	47048.0
24	49976.5
25	52905.0
26	52905.0
27	55898.0
28	58891.0
29	60496.5
30	62102.0
31	65580.0
32	69058.0
33	69058.0
34	73080.5
35	77103.0
36	84019.0
37	90935.0
38	93614.0
39	96293.0
40	96293.0
41	101423.0
42	106553.0
43	127902.0
44	149251.0
45	203007.0
46	256763.0
47	256763.0
48	261285.0
49	265807.0
50	237727.0
51	209647.0
52	191147.5
53	172648.0
54	172648.0
55	145163.5
56	117679.0
57	90709.5
58	63740.0
59	54017.0
60	44294.0
61	44294.0
62	38569.0
63	32844.0
64	28483.5
65	24123.0
66	20558.5
67	16994.0
68	16994.0
69	14381.0
70	11768.0
71	9730.0
72	7692.0
73	6027.0
74	4362.0
75	4362.0
76	3455.5
77	2549.0
78	1979.0
79	1409.0
80	1085.0
81	761.0
82	761.0
83	560.5
84	360.0
85	286.5
86	213.0
87	159.5
88	106.0
89	106.0
90	86.5
91	67.0
92	44.5
93	22.0
94	13.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2347726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.656654526926616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4695524158527	25.483651396861333
2	14.098751066307283	10.05428592064914
3	5.544706357904451	5.931170371711575
4	2.8290616292278963	4.03499492595053
5	1.570517605055208	2.7999701835954864
6	0.9787022242680059	2.0938348257275363
7	0.6140654907788483	1.5326864743126325
8	0.4504351918448517	1.284880961790543
9	0.322918652157817	1.036277893826286
>10	1.8197301497574234	12.260852598203586
>50	0.17081071815065507	4.2477396818442985
>100	0.10681189607446699	7.369973287140817
>500	0.010813998138202531	2.6311603098827803
>1k	0.010935503735260987	8.047240754775114
>5k	0.0010935503735260987	2.8401592945014187
>10k+	0.0010935503735260987	8.351121119226839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41712	1.7766979621983143	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37597	1.6014219717292393	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31846	1.3564615291562985	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19568	0.8334873831102948	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13837	0.589378828704883	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13430	0.5720429044956694	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12550	0.534559825124397	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11493	0.4895375354704936	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10723	0.45673984102063014	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9261	0.3944668159742662	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8499	0.36200987679141433	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8496	0.361882093566285	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8267	0.3521279740480789	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6862	0.2922828302791723	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6663	0.28380654301225955	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6577	0.2801434238918851	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5820	0.24789945675091554	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5110	0.21765742680363892	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4824	0.2054754260079754	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4824	0.2054754260079754	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4427	0.18856544588252633	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4394	0.1871598304061036	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4245	0.18081326355801317	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3751	0.15977162582004883	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3660	0.15589553465779227	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3658	0.15581034584103937	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3245	0.13821885518156718	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3161	0.1346409248779457	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3149	0.13412979197742836	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3085	0.13140374984133582	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2951	0.12569609911889207	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2930	0.12480161654298672	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2902	0.12360897310844622	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2891	0.1231404346163053	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2836	0.12079774215560078	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	2737	0.11658089572633262	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	2696	0.11483452498289835	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2681	0.11419560885725165	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2664	0.11347150391485207	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2614	0.11134178349602977	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	2600	0.11074546177875953	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2585	0.11010654565311284	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2501	0.10652861534949137	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	2497	0.10635823771598561	No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA	2456	0.1046118669725513	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	2425	0.10329144031288148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.2594408376445974E-5	0.0
2	4.2594408376445974E-5	0.0	0.0	4.2594408376445974E-5	0.0
3	4.2594408376445974E-5	0.0	0.0	8.518881675289195E-5	0.0
4	4.2594408376445974E-5	0.0	0.0	8.518881675289195E-5	0.0
5	4.2594408376445974E-5	0.0	0.0	8.518881675289195E-5	0.0
6	4.2594408376445974E-5	0.0	0.0	8.518881675289195E-5	0.0
7	4.2594408376445974E-5	0.0	0.0	2.1297204188222986E-4	0.0
8	4.2594408376445974E-5	0.0	0.0	2.1297204188222986E-4	0.0
9	4.2594408376445974E-5	0.0	0.0	3.8334967538801375E-4	0.0
10	4.2594408376445974E-5	0.0	0.0	6.389161256466896E-4	0.0
11	4.2594408376445974E-5	0.0	0.0	7.666993507760275E-4	0.0
12	4.2594408376445974E-5	0.0	0.0	0.0010222658010347033	0.0
13	4.2594408376445974E-5	0.0	0.0	0.0013204266596698253	0.0
14	4.2594408376445974E-5	0.0	0.0	0.001661181926681393	0.0
15	8.518881675289195E-5	0.0	0.0	0.0023000980523280824	0.0
16	8.518881675289195E-5	0.0	0.0	0.003450147078492124	0.0
17	8.518881675289195E-5	0.0	0.0	0.0048983569632912866	0.0
18	8.518881675289195E-5	0.0	0.0	0.005452084272185085	0.0
19	8.518881675289195E-5	0.0	0.0	0.00655953888997268	0.0
20	8.518881675289195E-5	0.0	0.0	0.007794776732889613	0.0
21	8.518881675289195E-5	0.0	0.0	0.010350441235476371	0.0
22	8.518881675289195E-5	0.0	0.0	0.016143280774673023	0.0
23	8.518881675289195E-5	0.0	0.0	0.02611037233476138	0.0
24	8.518881675289195E-5	0.0	0.0	0.039868366240353434	0.0
25	8.518881675289195E-5	0.0	0.0	0.04757795415649015	0.0
26	8.518881675289195E-5	0.0	0.0	0.05839693388410743	0.0
27	8.518881675289195E-5	0.0	0.0	0.1231404346163053	0.0
28	8.518881675289195E-5	0.0	0.0	0.1906951663013486	0.0
29	8.518881675289195E-5	0.0	0.0	0.2701337379234204	0.0
30	8.518881675289195E-5	0.0	0.0	0.4290534755759403	0.0
31	8.518881675289195E-5	0.0	0.0	0.6335492301912574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8950	0.0	29.68268	1
GTATTGG	790	0.0	25.29114	1
ATTGGAC	880	0.0	24.806818	3
TATTGGA	950	0.0	22.005262	2
ATCACGT	110	1.7571438E-9	20.181818	23
TTGGACC	1705	0.0	19.530792	4
CGTCTGT	870	0.0	19.350576	34
GGACCCT	1730	0.0	18.713875	6
TGGACCC	1780	0.0	18.396069	5
AGTATCA	490	0.0	18.122448	1
ACGTTTA	125	8.591087E-9	17.76	26
GTATCAA	15650	0.0	17.613419	2
ATAACGC	75	2.0684228E-4	17.266666	3
TCACGTT	130	1.3951649E-8	17.076923	24
TTTTTAC	1975	0.0	16.954432	1
CGATCTG	265	0.0	16.754717	33
GACCCTC	1940	0.0	16.402061	7
GCGTCTG	1020	0.0	16.32353	33
GCGCGTG	115	1.2434684E-6	16.086956	33
AACGGTA	70	0.0025935727	15.857143	18
>>END_MODULE