FastQCFastQC Report
Wed 25 May 2016
SRR2088224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431919
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52691.21990465804931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50051.1587820864560254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43130.9985668609160513No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG25140.5820535794905989No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.47995110194272533No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT15020.34775038838300704No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14850.3438144652122273No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT13120.3037606588272338No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12160.2815342691569484No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA10820.2505099335755084No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT10330.23916521384796685No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT10210.2363869151391812No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG9560.2213377971332588No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9060.20976155251331846No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8600.19911140746297337No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT6930.16044675043237275No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.16021522553997392No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA6580.1523433791984145No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6410.1484074560276348No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC5860.13567358694570048No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.1259495414649506No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA5370.12432886721815896No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC5290.1224766680789685No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT5120.1185407449081888No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.11252109770581985No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG4750.10997432388943297No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG4680.10835364964264134No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG4520.10464925136426044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGA303.595792E-430.83333210
CCTATAG250.00549259229.61
GGTATCA11700.029.5683771
AGGGTAC358.8603975E-426.428575
TGCGTTC451.3213445E-424.66666632
TACCCTA705.089765E-621.1428575
GGGTAGA450.003821999520.5555551
TGTACTG759.243828E-619.7333345
GTATTGG1153.0340743E-919.3043461
GGACGAC500.007027947318.525
GTGTAGA752.063504E-417.2666661
TCAAGAC752.063504E-417.2666663
GTATCAA20600.017.2427182
TTGGACC2050.016.2439044
TATACTG803.376453E-416.18755
TGGACCC2100.015.8571445
GCGTTCT700.002588982515.85714233
TTTGGAC1302.5778354E-715.6538463
ATTGGAC1302.5778354E-715.6538463
TAATACT957.043378E-515.5789484
Produced by FastQC (version 0.11.5)