##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088224_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431919
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.940023476624088	31.0	30.0	33.0	27.0	34.0
2	30.79864511632968	31.0	31.0	34.0	27.0	34.0
3	30.739048293777305	31.0	31.0	34.0	27.0	34.0
4	34.946221397993604	35.0	35.0	37.0	32.0	37.0
5	34.86819287875736	35.0	35.0	37.0	32.0	37.0
6	34.99983330207747	36.0	35.0	37.0	32.0	37.0
7	34.54626446162359	35.0	35.0	37.0	30.0	37.0
8	34.81008013076526	35.0	35.0	37.0	32.0	37.0
9	36.21009957885622	38.0	35.0	39.0	32.0	39.0
10	36.177568016225266	38.0	35.0	39.0	32.0	39.0
11	36.27480615578384	38.0	35.0	39.0	32.0	39.0
12	36.01843169668387	38.0	35.0	39.0	30.0	39.0
13	36.173551059342145	38.0	35.0	39.0	32.0	39.0
14	37.080913319395535	39.0	36.0	40.0	31.0	41.0
15	37.13829213347873	39.0	36.0	40.0	31.0	41.0
16	37.05463987460612	39.0	36.0	40.0	31.0	41.0
17	37.150817630157505	39.0	36.0	40.0	31.0	41.0
18	37.16179885580398	39.0	36.0	40.0	31.0	41.0
19	37.24542333168951	39.0	36.0	40.0	31.0	41.0
20	37.21216709614534	39.0	36.0	40.0	31.0	41.0
21	37.160229117033516	39.0	36.0	40.0	31.0	41.0
22	37.07874161590483	39.0	36.0	40.0	31.0	41.0
23	36.9452073189649	39.0	35.0	40.0	31.0	41.0
24	36.858600802465276	39.0	35.0	40.0	31.0	41.0
25	36.77127887404814	38.0	35.0	40.0	30.0	41.0
26	36.516467207971864	38.0	35.0	40.0	30.0	41.0
27	36.363077336259806	38.0	35.0	40.0	30.0	41.0
28	36.201713747253535	38.0	35.0	40.0	30.0	41.0
29	36.035812270356246	38.0	35.0	40.0	29.0	41.0
30	35.867384856882886	38.0	34.0	40.0	29.0	41.0
31	35.60650492337684	38.0	34.0	40.0	28.0	41.0
32	35.341401975833435	38.0	34.0	40.0	27.0	41.0
33	35.47254925113273	38.0	34.0	40.0	27.0	41.0
34	35.449778777965314	38.0	34.0	40.0	27.0	41.0
35	35.357194288743955	38.0	34.0	40.0	27.0	41.0
36	35.27281272646029	38.0	34.0	40.0	26.0	41.0
37	35.0980994121583	38.0	34.0	40.0	25.0	41.0
38	34.97450447884904	38.0	33.0	40.0	25.0	41.0
39	34.9100271115649	38.0	33.0	40.0	25.0	41.0
40	34.75679467677968	38.0	33.0	40.0	24.0	41.0
41	34.5955375892239	38.0	33.0	40.0	24.0	41.0
42	34.38499348257428	38.0	33.0	40.0	23.0	41.0
43	34.03564557243372	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	6.0
17	20.0
18	32.0
19	87.0
20	194.0
21	383.0
22	615.0
23	1130.0
24	1885.0
25	2950.0
26	4356.0
27	6336.0
28	8762.0
29	11848.0
30	15425.0
31	18573.0
32	22324.0
33	27239.0
34	32480.0
35	38484.0
36	46854.0
37	58949.0
38	68219.0
39	64764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.985087481680594	25.375591256693962	13.787538867241311	21.85178239438413
2	17.921878870806797	23.27589200752919	35.74304441342011	23.059184708243908
3	20.049824156844224	26.895089125507326	31.746924770616715	21.308161947031735
4	13.191825319099182	16.037729296465308	35.51082494634411	35.259620438091396
5	11.321798763194025	40.52287581699346	34.68034515730959	13.474980262502923
6	31.511232430154728	36.16719801629472	15.498507822068488	16.82306173148206
7	24.067707139533105	35.188773821017364	22.144892908161022	18.598626131288505
8	27.566511313463867	33.82161933140242	20.412160613448354	18.199708741685363
9	25.003067704824282	13.843567891201822	23.852852039387017	37.30051236458688
10	16.22526445930834	28.077718275880432	34.083010934920665	21.614006329890557
11	31.27021501716757	21.897392798186697	25.065347900879566	21.767044283766168
12	19.459435681227266	27.68019003563168	32.15093570785263	20.709438575288424
13	30.28692879914984	21.432722339142295	29.059152294758974	19.221196566948894
14	20.603168649677368	22.878595292172836	28.855178864555626	27.66305719359417
15	22.852201454439374	29.10175287496035	27.192135562455	20.853910108145275
16	21.525100771209416	28.56716189841151	27.54636864782517	22.361368682553906
17	19.028799381365488	27.430374676733372	28.607447229688898	24.933378712212242
18	20.068809198020926	26.795764946668243	32.5060948927924	20.629330962518434
19	19.469159726708014	25.386935976421505	33.86653516052778	21.277369136342696
20	23.623179346127397	24.400176885017792	32.69594530455942	19.28069846429539
21	22.865398373306107	25.2906216211836	31.510537855477533	20.33344215003276
22	21.300290100690177	24.853039574549857	32.49428712328006	21.352383201479906
23	20.431840229302253	26.853877694660344	33.251836571208955	19.46244550482845
24	19.35802777835659	26.011821661005886	31.81013106624159	22.82001949439594
25	20.257270460433556	27.042570481965367	32.773274618620626	19.92688443898046
26	21.874471833839216	25.997235592784758	31.40797232814486	20.720320245231168
27	20.538573204698103	27.394719843303957	31.007434264294925	21.059272687703018
28	20.06603089931214	26.785346326510297	31.45450883151702	21.694113942660547
29	19.410583928931118	26.511683903694905	32.396583618687764	21.681148548686213
30	18.641921286167083	26.790208349250673	34.12098101727407	20.446889347308176
31	20.08200611688766	26.453802680595206	33.0298041993985	20.43438700311864
32	21.03380494953915	26.701765840354323	31.153294946506172	21.111134263600352
33	21.99949527573457	26.50473815692294	30.65783167677273	20.83793489056976
34	20.21513293001697	29.016551714557593	30.747663335023468	20.020652020401975
35	19.110990718167066	29.880371088097533	30.846061414292958	20.162576779442443
36	20.12414364730424	26.351237153262534	32.75058517916554	20.77403402026769
37	20.03871096200908	26.74691319437209	32.12014289716359	21.09423294645524
38	20.19962076222625	26.42625121839975	32.578330659220825	20.795797360153177
39	21.125720331821476	25.792567587904212	32.52971043181708	20.55200164845723
40	19.73078285511867	26.48112261789826	31.603147812437054	22.184946714546015
41	20.58557275785506	26.097022821408643	31.37787409213302	21.93953032860328
42	18.39372660151556	27.588506178241758	31.748082395078708	22.269684825163978
43	18.976474755683356	27.231494794162796	31.54341439019816	22.248616059955687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	62.0
2	74.0
3	195.0
4	316.0
5	316.0
6	558.5
7	801.0
8	901.5
9	1002.0
10	1508.0
11	2014.0
12	2014.0
13	3816.5
14	5619.0
15	8715.5
16	11812.0
17	11322.0
18	10832.0
19	10832.0
20	11517.5
21	12203.0
22	10005.5
23	7808.0
24	8716.5
25	9625.0
26	9625.0
27	10464.0
28	11303.0
29	11831.0
30	12359.0
31	13352.0
32	14345.0
33	14345.0
34	15655.5
35	16966.0
36	18308.0
37	19650.0
38	20522.5
39	21395.0
40	21395.0
41	22478.5
42	23562.0
43	26167.5
44	28773.0
45	35390.5
46	42008.0
47	42008.0
48	42305.5
49	42603.0
50	38671.0
51	34739.0
52	31990.0
53	29241.0
54	29241.0
55	25611.5
56	21982.0
57	18165.5
58	14349.0
59	12593.0
60	10837.0
61	10837.0
62	9616.5
63	8396.0
64	7162.0
65	5928.0
66	5003.5
67	4079.0
68	4079.0
69	3486.0
70	2893.0
71	2354.5
72	1816.0
73	1470.0
74	1124.0
75	1124.0
76	865.0
77	606.0
78	486.0
79	366.0
80	292.5
81	219.0
82	219.0
83	156.0
84	93.0
85	80.0
86	67.0
87	52.5
88	38.0
89	38.0
90	29.5
91	21.0
92	12.0
93	3.0
94	2.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	431919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.14861564425361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65184304014271	52.37491629283625
2	8.63087693752869	10.55532353051589
3	2.303406958030434	4.225504402467073
4	0.9989537422651663	2.4433855372864577
5	0.5462708580966879	1.6701853369705482
6	0.3580964369489155	1.3138260831939559
7	0.272578239197531	1.1667447387174072
8	0.191775893362212	0.9381464314431416
9	0.14152652627180795	0.7788736042615031
>10	0.8018458518363242	8.703616125487015
>50	0.053697769635856325	2.2742619434625735
>100	0.03998770078903746	5.185904076943271
>500	0.0045700229473185664	1.8895516493923257
>1k	0.003808352456098806	4.087197559586482
>5k	7.616704912197611E-4	2.3925626874361297
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5269	1.21990465804931	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5005	1.1587820864560254	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4313	0.9985668609160513	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2514	0.5820535794905989	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2073	0.47995110194272533	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1502	0.34775038838300704	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1485	0.3438144652122273	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1312	0.3037606588272338	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1216	0.2815342691569484	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1082	0.2505099335755084	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1033	0.23916521384796685	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1021	0.2363869151391812	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	956	0.2213377971332588	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	906	0.20976155251331846	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	860	0.19911140746297337	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	693	0.16044675043237275	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	692	0.16021522553997392	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	658	0.1523433791984145	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	641	0.1484074560276348	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	586	0.13567358694570048	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	544	0.1259495414649506	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	537	0.12432886721815896	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	529	0.1224766680789685	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	512	0.1185407449081888	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.11252109770581985	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	475	0.10997432388943297	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	468	0.10835364964264134	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	452	0.10464925136426044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.3152489239880626E-4	0.0
6	0.0	0.0	0.0	2.3152489239880626E-4	0.0
7	0.0	0.0	0.0	2.3152489239880626E-4	0.0
8	0.0	0.0	0.0	2.3152489239880626E-4	0.0
9	0.0	0.0	0.0	2.3152489239880626E-4	0.0
10	0.0	0.0	0.0	4.630497847976125E-4	0.0
11	0.0	0.0	0.0	0.0013891493543928375	0.0
12	0.0	0.0	0.0	0.0016206742467916439	0.0
13	0.0	0.0	0.0	0.0023152489239880625	0.0
14	0.0	0.0	0.0	0.002778298708785675	0.0
15	0.0	0.0	0.0	0.003472873385982094	0.0
16	0.0	0.0	0.0	0.006714221879565382	0.0
17	0.0	0.0	0.0	0.009724045480749862	0.0
18	0.0	0.0	0.0	0.010418620157946281	0.0
19	0.0	0.0	0.0	0.01180776951233912	0.0
20	0.0	0.0	0.0	0.013428443759130763	0.0
21	0.0	0.0	0.0	0.01736436692991047	0.0
22	0.0	0.0	0.0	0.02778298708785675	0.0
23	0.0	0.0	0.0	0.04584192869496364	0.0
24	0.0	0.0	0.0	0.06552154454886217	0.0
25	0.0	0.0	0.0	0.07293034110562398	0.0
26	0.0	0.0	0.0	0.08358048615596905	0.0
27	0.0	0.0	0.0	0.15072270495162288	0.0
28	0.0	0.0	0.0	0.2180964486396755	0.0
29	0.0	0.0	0.0	0.2898691652833054	0.0
30	0.0	0.0	0.0	0.4357298474945534	0.0
31	0.0	0.0	0.0	0.6177084129200151	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCGA	30	3.595792E-4	30.833332	10
CCTATAG	25	0.005492592	29.6	1
GGTATCA	1170	0.0	29.568377	1
AGGGTAC	35	8.8603975E-4	26.42857	5
TGCGTTC	45	1.3213445E-4	24.666666	32
TACCCTA	70	5.089765E-6	21.142857	5
GGGTAGA	45	0.0038219995	20.555555	1
TGTACTG	75	9.243828E-6	19.733334	5
GTATTGG	115	3.0340743E-9	19.304346	1
GGACGAC	50	0.0070279473	18.5	25
GTGTAGA	75	2.063504E-4	17.266666	1
TCAAGAC	75	2.063504E-4	17.266666	3
GTATCAA	2060	0.0	17.242718	2
TTGGACC	205	0.0	16.243904	4
TATACTG	80	3.376453E-4	16.1875	5
TGGACCC	210	0.0	15.857144	5
GCGTTCT	70	0.0025889825	15.857142	33
TTTGGAC	130	2.5778354E-7	15.653846	3
ATTGGAC	130	2.5778354E-7	15.653846	3
TAATACT	95	7.043378E-5	15.578948	4
>>END_MODULE