FastQCFastQC Report
Wed 25 May 2016
SRR2088223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856228
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82630.9650466931705107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT77850.9092204412843308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54720.6390821136426279No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30360.35457845340259836No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17020.1987788299378203No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT14840.17331832175542028No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT12360.14435407391489183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11040.12893761941912668No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA10510.12274767935643309No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT10400.12146297481511933No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10100.1179592351569909No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.11620736532792666No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG9050.10569614635354135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21800.024.185781
CGATTAG450.003825023320.5555551
AGCCGTC609.2341914E-418.512
ACGTTTA701.21873614E-418.526
TCTAGAC801.6158861E-518.53
CGGTATA609.2341914E-418.526
GACTGAT752.0664906E-417.26666611
CGAAGGT752.0664906E-417.2666664
ATTAGAC1107.801882E-716.8181823
CATAGGG803.3813057E-416.18752
TCACGTT803.3813057E-416.187524
GTATCAA34400.015.97238352
CGATCTG700.00259176915.85714333
AACTGCG957.056312E-515.5789487
GATTAAT1557.197741E-915.51612951
CAGTGCG855.362166E-415.2352949
ATCTCCG750.00410326914.810
AGTACCC750.00410326914.83
TTAGACT1551.2086639E-714.3225814
CATACAC1551.2086639E-714.3225813
Produced by FastQC (version 0.11.5)