##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088223_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	856228
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.7459555165213	31.0	30.0	31.0	26.0	34.0
2	30.572513395964627	31.0	30.0	34.0	27.0	34.0
3	30.573521305072948	31.0	30.0	34.0	27.0	34.0
4	34.73539290936526	35.0	35.0	37.0	32.0	37.0
5	34.57064239898718	35.0	35.0	37.0	30.0	37.0
6	34.688911131147314	35.0	35.0	37.0	31.0	37.0
7	34.28281135398515	35.0	35.0	37.0	30.0	37.0
8	34.508321381688056	35.0	35.0	37.0	30.0	37.0
9	35.94570605025764	37.0	35.0	39.0	30.0	39.0
10	35.80073882190258	37.0	35.0	39.0	30.0	39.0
11	35.92301232849194	37.0	35.0	39.0	30.0	39.0
12	35.646929322563615	37.0	35.0	39.0	30.0	39.0
13	35.8384787696735	37.0	35.0	39.0	30.0	39.0
14	36.69723484866181	38.0	36.0	40.0	31.0	41.0
15	36.75597387611711	38.0	36.0	40.0	31.0	41.0
16	36.66261322918662	38.0	35.0	40.0	30.0	41.0
17	36.71415323955769	38.0	36.0	40.0	31.0	41.0
18	36.701582989577545	38.0	36.0	40.0	31.0	41.0
19	36.779768940048676	39.0	35.0	40.0	30.0	41.0
20	36.74622997612786	39.0	35.0	40.0	30.0	41.0
21	36.67862064777139	38.0	35.0	40.0	30.0	41.0
22	36.60011352116492	38.0	35.0	40.0	30.0	41.0
23	36.47684612042587	38.0	35.0	40.0	30.0	41.0
24	36.393895084019675	38.0	35.0	40.0	30.0	41.0
25	36.28953619830232	38.0	35.0	40.0	30.0	41.0
26	36.02452968134656	38.0	34.0	40.0	29.0	41.0
27	35.87643828512966	38.0	34.0	40.0	29.0	41.0
28	35.72791008936872	38.0	34.0	40.0	27.0	41.0
29	35.54321045329048	38.0	34.0	40.0	27.0	41.0
30	35.36944482077204	38.0	34.0	40.0	27.0	41.0
31	35.162253511915054	38.0	33.0	40.0	27.0	41.0
32	34.89100683462816	37.0	33.0	40.0	26.0	41.0
33	35.08233788196602	37.0	33.0	40.0	26.0	41.0
34	35.086795806724375	38.0	33.0	40.0	26.0	41.0
35	35.024911588969296	38.0	33.0	40.0	26.0	41.0
36	34.951591164970075	38.0	33.0	40.0	25.0	41.0
37	34.8003242127097	37.0	33.0	40.0	25.0	41.0
38	34.68457583727699	37.0	33.0	40.0	25.0	41.0
39	34.67393264410881	38.0	33.0	40.0	24.0	41.0
40	34.55756410675661	38.0	33.0	40.0	24.0	41.0
41	34.42659315042255	38.0	33.0	40.0	24.0	41.0
42	34.267698556926426	37.0	33.0	40.0	23.0	41.0
43	33.9050836926613	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	10.0
16	13.0
17	55.0
18	118.0
19	254.0
20	517.0
21	926.0
22	1760.0
23	2884.0
24	4838.0
25	7015.0
26	10498.0
27	14935.0
28	19929.0
29	26240.0
30	33265.0
31	40383.0
32	48247.0
33	57083.0
34	67756.0
35	79878.0
36	95377.0
37	115072.0
38	134795.0
39	94379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.942347131838716	26.106597775358896	13.995220899106311	21.955834193696074
2	18.981626389232776	24.868843345464057	33.66217876546901	22.48735149983416
3	20.427386163498507	26.79169566984495	31.676726292529562	21.104191874126986
4	14.917288385803781	18.17740134637036	35.744684826938624	31.16062544088724
5	12.19640095862317	40.26953101276763	34.60865563845144	12.925412390157762
6	31.114492868721882	37.930901582288826	15.687176780016538	15.26742876897275
7	24.488454009913248	34.661094942001434	22.88479236838786	17.965658679697462
8	26.274777278949063	34.90530559617298	20.764796292576275	18.05512083230168
9	25.04321278911692	14.275753654400464	23.33864344543743	37.34239011104519
10	15.932438556085529	28.970554571913087	34.70185511335765	20.395151758643728
11	32.007128942291075	23.511027436617347	23.892234311421724	20.589609309669854
12	19.221632555814573	28.554543883171306	31.77284555048422	20.450978010529905
13	29.284606436603333	22.111049860551162	27.97642683958011	20.62791686326539
14	20.46195639479204	23.686214419523772	28.69913153973007	27.152697645954117
15	23.22290324539725	30.151198045380433	25.641301148759442	20.984597560462866
16	21.62800095301719	28.760680566391194	27.663542888109244	21.947775592482376
17	19.805122000214894	28.789294440265913	28.79910491130867	22.606478648210523
18	20.122444021919396	27.613205828354133	31.27075965747441	20.993590492252064
19	20.22592113315612	27.130040129498216	32.57485155822982	20.069187179115843
20	22.13207229849993	26.526228995080746	31.671937848330117	19.669760858089198
21	21.829349192037633	26.971320722985	30.804762282943326	20.39456780203404
22	21.350855146059228	27.03754140252363	31.114259286078006	20.497344165339136
23	20.885324936815895	27.909738994753734	31.165647467730555	20.039288600699816
24	20.190066197321276	27.59942445236549	30.796937264373508	21.413572085939727
25	20.836973329533723	27.958557767323654	31.23105060801562	19.973418295126997
26	21.164572987568732	27.55574449796082	30.548989287899953	20.730693226570494
27	20.715393563396663	28.050122163722747	30.409657240828373	20.82482703205221
28	20.579682047305155	28.290712286914232	30.597107312538245	20.53249835324236
29	19.973418295126997	27.88439527789327	31.217035649383106	20.92515077759662
30	19.922847652728013	28.28849325179742	31.704873001116525	20.083786094358043
31	20.701845770051904	27.86967957132913	31.097090961753178	20.33138369686579
32	20.66575725157318	28.421985732772114	30.43908865395666	20.47316836169805
33	21.065533946565633	28.30052275795699	30.19312612995604	20.440817165521334
34	20.533315892495924	28.91741452043147	30.420985999056327	20.128283588016277
35	19.973768669092813	29.246649256973612	30.381627323563347	20.39795475037023
36	20.42972198993726	27.976660422223986	31.030636699570675	20.56298088826808
37	20.243439831446764	27.66646267115768	31.15326758760517	20.936829909790383
38	20.41500628337312	27.763983424975592	31.16658179830606	20.654428493345232
39	20.73279547036537	27.39071836006297	31.283957076853362	20.592529092718294
40	20.012193014010286	27.443040872291025	31.266204795918846	21.278561317779843
41	20.171963542420944	27.11964570184577	31.189472897405828	21.518917858327455
42	19.153543215124945	27.99464628580238	30.919801735051877	21.9320087640208
43	19.30829171669228	27.502604446479207	31.18083033958245	22.008273497246062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	208.0
1	215.0
2	222.0
3	439.5
4	657.0
5	657.0
6	1109.5
7	1562.0
8	1770.0
9	1978.0
10	2987.5
11	3997.0
12	3997.0
13	6803.5
14	9610.0
15	14615.0
16	19620.0
17	18697.5
18	17775.0
19	17775.0
20	20145.5
21	22516.0
22	20016.5
23	17517.0
24	19647.0
25	21777.0
26	21777.0
27	24154.5
28	26532.0
29	28708.0
30	30884.0
31	33323.0
32	35762.0
33	35762.0
34	38554.0
35	41346.0
36	43909.0
37	46472.0
38	47630.0
39	48788.0
40	48788.0
41	50684.5
42	52581.0
43	55472.5
44	58364.0
45	63424.0
46	68484.0
47	68484.0
48	68918.5
49	69353.0
50	63443.5
51	57534.0
52	53190.0
53	48846.0
54	48846.0
55	44571.0
56	40296.0
57	36029.5
58	31763.0
59	27972.5
60	24182.0
61	24182.0
62	21156.0
63	18130.0
64	15689.5
65	13249.0
66	11168.0
67	9087.0
68	9087.0
69	7784.0
70	6481.0
71	5356.5
72	4232.0
73	3424.0
74	2616.0
75	2616.0
76	2133.0
77	1650.0
78	1317.5
79	985.0
80	777.5
81	570.0
82	570.0
83	428.0
84	286.0
85	220.0
86	154.0
87	123.5
88	93.0
89	93.0
90	72.0
91	51.0
92	33.0
93	15.0
94	8.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	856228.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.58642290863526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.257138803337	45.641351136573704
2	11.967788053215504	13.783642082267614
3	3.9181453957680086	6.768959333346558
4	1.653137978894663	3.8079321111581845
5	0.8590481108299691	2.4734753904559392
6	0.4984891186543087	1.7223723121307932
7	0.32054851187612804	1.292146951734268
8	0.2521103222006842	1.1614505307104717
9	0.16675559092606068	0.8642572183302761
>10	0.9866066917772766	10.614559022440481
>50	0.07783409646186033	3.085016619263608
>100	0.03809614806387599	4.01787680907214
>500	0.002048179997409882	0.8531132514360282
>1k	0.0016385439979279058	1.3756200541266967
>5k	6.144539992229646E-4	2.538227176953315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8263	0.9650466931705107	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7785	0.9092204412843308	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5472	0.6390821136426279	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3036	0.35457845340259836	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1702	0.1987788299378203	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1484	0.17331832175542028	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1236	0.14435407391489183	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1104	0.12893761941912668	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1051	0.12274767935643309	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1040	0.12146297481511933	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1010	0.1179592351569909	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	995	0.11620736532792666	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	905	0.10569614635354135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.1679132193761475E-4	0.0	0.0	0.0	0.0
9	2.335826438752295E-4	0.0	0.0	0.0	0.0
10	2.335826438752295E-4	0.0	0.0	0.0	0.0
11	2.335826438752295E-4	0.0	0.0	0.0	0.0
12	2.335826438752295E-4	0.0	0.0	1.1679132193761475E-4	0.0
13	2.335826438752295E-4	0.0	0.0	2.335826438752295E-4	0.0
14	2.335826438752295E-4	0.0	0.0	2.335826438752295E-4	0.0
15	2.335826438752295E-4	0.0	0.0	3.5037396581284427E-4	0.0
16	2.335826438752295E-4	0.0	0.0	4.67165287750459E-4	0.0
17	2.335826438752295E-4	0.0	0.0	0.0010511218974385327	0.0
18	2.335826438752295E-4	0.0	0.0	0.0012847045413137623	0.0
19	2.335826438752295E-4	0.0	0.0	0.001401495863251377	0.0
20	2.335826438752295E-4	0.0	0.0	0.001401495863251377	0.0
21	2.335826438752295E-4	0.0	0.0	0.0021022437948770654	0.0
22	2.335826438752295E-4	0.0	0.0	0.0033869483361908276	0.0
23	2.335826438752295E-4	0.0	0.0	0.007007479316256885	0.0
24	2.335826438752295E-4	0.0	0.0	0.013664584666700926	0.0
25	2.335826438752295E-4	0.0	0.0	0.01646757639320368	0.0
26	2.335826438752295E-4	0.0	0.0	0.02464296892883671	0.0
27	2.335826438752295E-4	0.0	0.0	0.07416248943038536	0.0
28	2.335826438752295E-4	0.0	0.0	0.12298126200030833	0.0
29	2.335826438752295E-4	0.0	0.0	0.1823112535446166	0.0
30	2.335826438752295E-4	0.0	0.0	0.2693207883881396	0.0
31	2.335826438752295E-4	0.0	0.0	0.41928084575603697	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2180	0.0	24.18578	1
CGATTAG	45	0.0038250233	20.555555	1
AGCCGTC	60	9.2341914E-4	18.5	12
ACGTTTA	70	1.21873614E-4	18.5	26
TCTAGAC	80	1.6158861E-5	18.5	3
CGGTATA	60	9.2341914E-4	18.5	26
GACTGAT	75	2.0664906E-4	17.266666	11
CGAAGGT	75	2.0664906E-4	17.266666	4
ATTAGAC	110	7.801882E-7	16.818182	3
CATAGGG	80	3.3813057E-4	16.1875	2
TCACGTT	80	3.3813057E-4	16.1875	24
GTATCAA	3440	0.0	15.9723835	2
CGATCTG	70	0.002591769	15.857143	33
AACTGCG	95	7.056312E-5	15.578948	7
GATTAAT	155	7.197741E-9	15.5161295	1
CAGTGCG	85	5.362166E-4	15.235294	9
ATCTCCG	75	0.004103269	14.8	10
AGTACCC	75	0.004103269	14.8	3
TTAGACT	155	1.2086639E-7	14.322581	4
CATACAC	155	1.2086639E-7	14.322581	3
>>END_MODULE