FastQCFastQC Report
Wed 25 May 2016
SRR2088222_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088222_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599920
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157022.6173489798639817No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT119541.9925990132017604No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71891.1983264435258034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48950.8159421256167488No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT32760.546072809707961No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT32520.5420722763035071No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG21260.35438058407787704No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19980.33304440592078943No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG19000.31670889451926926No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18250.30420722763035074No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA17670.2945392719029204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17500.29170556074143217No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA16250.2708694492599013No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14560.24269902653687156No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14060.23436458194425924No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.18969195892785706No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC11220.1870249366582211No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11050.1841912254967329No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT10660.17769035871449526No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA10440.17402320309374583No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA9940.1656887585011335No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8740.14568609147886386No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8590.14318575810108014No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8200.1366848913188425No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT8060.13435124683291105No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.1291838911854914No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7670.12785038005067342No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7670.12785038005067342No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC7390.12318309107881051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC7230.12051606880917455No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC7170.11951593545806106No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC7030.11718229097212962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7010.11684891318842514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC6930.11551540205360716No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6790.11318175756767569No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA6620.11034804640618749No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG6610.11018135751433525No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA6210.10351380184024538No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG6020.10034671289505268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG352.3864186E-531.7142877
GGTATCA30200.031.670531
GTCGCGT358.8644313E-426.4285728
TATTGTG358.8644313E-426.4285725
CGCGTAA358.8644313E-426.42857210
GCGTAAC358.8644313E-426.42857211
CGTAACT400.001930116423.12500212
GTATCAA42750.023.0222242
ATACCGT603.7224647E-521.5833346
CGGGGGT450.00382370920.5555553
TCGCGTA450.00382370920.5555559
GTATTGG1001.2867531E-820.3499981
GTTGACT555.1391555E-420.1818184
TAGTCCT851.2432774E-619.5882364
ATTGGAC1153.0413503E-919.3043483
TCGTTCG500.00703105918.49999837
AAGCGGG500.00703105918.49999825
TCAGTAC500.00703105918.4999983
GTGGTAT3200.017.9218751
TATGAGT650.001578743717.0769234
Produced by FastQC (version 0.11.5)