##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088222_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599920
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.2466612214962	31.0	30.0	31.0	26.0	33.0
2	29.946486198159754	31.0	30.0	31.0	26.0	34.0
3	29.76888418455794	31.0	30.0	31.0	26.0	34.0
4	33.86055974129884	35.0	33.0	37.0	28.0	37.0
5	33.58448126416856	35.0	33.0	37.0	28.0	37.0
6	33.982752700360045	35.0	33.0	37.0	30.0	37.0
7	33.55436558207761	35.0	33.0	37.0	28.0	37.0
8	33.91163321776237	35.0	33.0	37.0	29.0	37.0
9	35.085788105080674	37.0	34.0	39.0	29.0	39.0
10	34.9172606347513	37.0	33.0	39.0	28.0	39.0
11	35.208509467929055	37.0	34.0	39.0	29.0	39.0
12	34.82285138018403	37.0	33.0	39.0	27.0	39.0
13	35.12030770769436	37.0	34.0	39.0	29.0	39.0
14	35.71864581944259	38.0	34.0	40.0	27.0	41.0
15	35.863835178023734	38.0	34.0	40.0	29.0	41.0
16	35.60471729563942	38.0	33.0	40.0	27.0	41.0
17	35.8096112815042	38.0	34.0	40.0	28.0	41.0
18	35.85987798373117	38.0	34.0	40.0	29.0	41.0
19	35.977648686491534	38.0	34.0	40.0	29.0	41.0
20	35.955035671422856	38.0	34.0	40.0	29.0	41.0
21	35.96759234564609	38.0	34.0	40.0	29.0	41.0
22	35.854193892519	38.0	34.0	40.0	28.0	41.0
23	35.647768035738096	38.0	34.0	40.0	27.0	41.0
24	35.53401786904921	38.0	34.0	40.0	27.0	41.0
25	35.39915155354047	37.0	33.0	40.0	27.0	41.0
26	35.06655720762768	37.0	33.0	40.0	27.0	41.0
27	34.81577210294706	37.0	33.0	40.0	26.0	41.0
28	34.566003800506735	37.0	33.0	39.0	25.0	41.0
29	34.35471729563942	36.0	33.0	39.0	25.0	40.0
30	34.087274969996	36.0	32.0	39.0	25.0	40.0
31	33.74400253367116	36.0	32.0	39.0	24.0	40.0
32	33.45752100280038	35.0	31.0	39.0	24.0	40.0
33	33.50698426456861	35.0	31.0	39.0	24.0	40.0
34	33.40049673289772	35.0	31.0	39.0	23.0	40.0
35	33.202735364715295	35.0	31.0	39.0	22.0	40.0
36	33.00013001733564	35.0	31.0	39.0	21.0	40.0
37	32.73896019469262	35.0	31.0	39.0	19.0	40.0
38	32.551540205360716	35.0	31.0	39.0	18.0	40.0
39	32.346689558607814	35.0	30.0	39.0	16.0	40.0
40	32.08485631417523	35.0	30.0	39.0	15.0	40.0
41	31.814128550473395	35.0	30.0	39.0	14.0	40.0
42	31.50487231630884	35.0	29.0	39.0	10.0	40.0
43	31.060384717962396	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	8.0
15	19.0
16	29.0
17	107.0
18	244.0
19	478.0
20	940.0
21	1575.0
22	2673.0
23	4157.0
24	6257.0
25	8838.0
26	12159.0
27	16322.0
28	20990.0
29	25663.0
30	30914.0
31	35994.0
32	41552.0
33	48189.0
34	54693.0
35	62541.0
36	71329.0
37	74323.0
38	58258.0
39	21664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.048039738631815	28.037238298439792	14.698126416855581	18.21659554607281
2	24.25973463128417	26.28017068942526	28.553807174289908	20.906287505000666
3	26.735731430857445	24.695459394585946	31.11848246432858	17.45032671022803
4	19.78930524069876	19.048039738631818	30.05884117882384	31.10381384184558
5	10.43922523003067	44.14855314041872	32.80904120549407	12.60318042405654
6	33.08591145486065	35.62875050006667	12.905554073876516	18.379783971196158
7	21.80790772102947	38.55547406320843	21.41768902520336	18.21892919055874
8	28.30344045872783	36.16748899853314	19.463095079343912	16.06597546339512
9	23.958361114815308	13.576643552473664	25.57924389918656	36.88575143352447
10	17.81320842779037	30.85761434857981	32.17412321642886	19.15505400720096
11	31.465695426056804	21.33684491265502	23.771002800373385	23.426456860914787
12	17.623349779970663	30.636918255767437	32.649519935991464	19.090212028270436
13	31.458861181490867	20.514401920256034	31.695392719029204	16.331344179223898
14	19.757967729030536	23.95986131484198	31.717895719429258	24.564275236698226
15	24.47826376850247	29.04003867182291	28.69432591012135	17.787371649553275
16	20.34854647286305	27.388985198026404	31.54253900520069	20.719929323909856
17	17.452993732497664	28.639485264701957	32.020269369249235	21.88725163355114
18	18.07040938791839	24.632617682357647	36.318009067875714	20.978963861848246
19	17.881550873449793	24.69662621682891	39.43909187891719	17.982731030804107
20	21.33601146819576	23.059907987731698	38.92669022536338	16.67739031870916
21	21.463361781570875	22.90438725163355	36.938091745566076	18.694159221229498
22	19.812641685558074	23.07057607681024	37.5446726230164	19.572109614615282
23	19.0840445392719	25.937958394452593	38.07774369915989	16.900253367115614
24	17.544672623016403	24.714961994932658	37.03560474729964	20.704760634751302
25	18.407454327243634	25.764768635818108	38.292105614081876	17.53567142285638
26	19.598279770636083	24.394919322576346	36.89058541138818	19.116215495399384
27	20.246199493265767	25.11701560208028	36.32484331244166	18.311941592212293
28	18.936191492198958	26.06297506334178	36.824076543539135	18.17675690092012
29	17.92455660754767	24.74179890652087	37.53817175623416	19.795472729697295
30	17.262468329110547	25.579743965862118	39.13105080677424	18.0267368982531
31	18.542472329643953	24.32857714361915	37.502833711161486	19.626116815575408
32	18.957360981464195	26.36334844645953	36.03763835178024	18.64165222029604
33	20.29370582744366	25.953627150286707	34.788138418455794	18.96452860381384
34	19.586611548206427	27.32714361914922	35.18852513668489	17.89771969595946
35	17.852880384051208	27.940392052273637	35.19102547006268	19.015702093612482
36	19.243065742098945	24.763635151353512	37.25046672889719	18.742832377650352
37	18.355780770769435	25.32954393919189	36.820909454593945	19.493765835444727
38	19.666455527403652	25.210361381517536	35.64725296706228	19.475930124016536
39	20.535238031737567	25.25870116015469	35.11234831310841	19.09371249499933
40	19.518935858114414	25.78760501400187	34.073876516868914	20.619582611014803
41	19.398753167088945	25.978630484064542	33.904353913855175	20.718262434991335
42	17.937725030004	26.831077476996935	33.154753967195624	22.07644352580344
43	18.38795172689692	26.45486064808641	33.24926656887585	21.907921056140818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	79.0
2	95.0
3	214.0
4	333.0
5	333.0
6	799.0
7	1265.0
8	1451.5
9	1638.0
10	2773.5
11	3909.0
12	3909.0
13	7945.0
14	11981.0
15	19526.5
16	27072.0
17	24280.0
18	21488.0
19	21488.0
20	25872.0
21	30256.0
22	22858.5
23	15461.0
24	15320.5
25	15180.0
26	15180.0
27	15822.5
28	16465.0
29	16758.0
30	17051.0
31	17506.5
32	17962.0
33	17962.0
34	19579.5
35	21197.0
36	22573.5
37	23950.0
38	24710.0
39	25470.0
40	25470.0
41	27074.0
42	28678.0
43	32833.5
44	36989.0
45	46260.5
46	55532.0
47	55532.0
48	57485.5
49	59439.0
50	53027.5
51	46616.0
52	41813.0
53	37010.0
54	37010.0
55	31566.0
56	26122.0
57	21773.0
58	17424.0
59	15115.0
60	12806.0
61	12806.0
62	11089.0
63	9372.0
64	8178.5
65	6985.0
66	5811.5
67	4638.0
68	4638.0
69	3808.0
70	2978.0
71	2485.0
72	1992.0
73	1572.5
74	1153.0
75	1153.0
76	899.5
77	646.0
78	495.5
79	345.0
80	265.5
81	186.0
82	186.0
83	136.5
84	87.0
85	68.0
86	49.0
87	34.0
88	19.0
89	19.0
90	17.0
91	15.0
92	8.0
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	599920.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.54384029303074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.26379442394636	45.118071511205585
2	9.088928506857469	9.733122728119014
3	2.6507832655394434	4.257993474644474
4	1.1722223875208375	2.5106115322132365
5	0.6257081470261605	1.6751408547208464
6	0.39335715755940304	1.2637111693488725
7	0.2936285308116259	1.1005399411478465
8	0.22704060910818313	0.9725300891296782
9	0.16657144603633817	0.8026987413553991
>10	0.977315214106158	9.68710191105851
>50	0.07126701143023155	2.569435068663463
>100	0.05462757561113479	5.821531175068536
>500	0.008476692767245036	3.1245069432300183
>1k	0.005337176927524653	5.505499913796004
>5k	3.1395158397203845E-4	1.2084833708979719
>10k+	6.279031679440769E-4	4.649021575400516
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15702	2.6173489798639817	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11954	1.9925990132017604	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7189	1.1983264435258034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4895	0.8159421256167488	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3276	0.546072809707961	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3252	0.5420722763035071	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2126	0.35438058407787704	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1998	0.33304440592078943	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1900	0.31670889451926926	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1825	0.30420722763035074	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1767	0.2945392719029204	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1750	0.29170556074143217	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1625	0.2708694492599013	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1456	0.24269902653687156	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1406	0.23436458194425924	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.18969195892785706	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1122	0.1870249366582211	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1105	0.1841912254967329	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1066	0.17769035871449526	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1044	0.17402320309374583	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	994	0.1656887585011335	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	874	0.14568609147886386	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	859	0.14318575810108014	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	820	0.1366848913188425	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	806	0.13435124683291105	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	775	0.1291838911854914	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	767	0.12785038005067342	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	767	0.12785038005067342	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	739	0.12318309107881051	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	723	0.12051606880917455	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	717	0.11951593545806106	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	703	0.11718229097212962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	701	0.11684891318842514	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	693	0.11551540205360716	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	679	0.11318175756767569	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	662	0.11034804640618749	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	661	0.11018135751433525	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	621	0.10351380184024538	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	602	0.10034671289505268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.6668889185224696E-4	0.0
12	0.0	0.0	0.0	1.6668889185224696E-4	0.0
13	0.0	0.0	0.0	1.6668889185224696E-4	0.0
14	0.0	0.0	0.0	3.333777837044939E-4	0.0
15	0.0	0.0	0.0	0.0011668222429657288	0.0
16	0.0	0.0	0.0	0.0018335778103747166	0.0
17	0.0	0.0	0.0	0.0033337778370449394	0.0
18	0.0	0.0	0.0	0.004167222296306174	0.0
19	0.0	0.0	0.0	0.005000666755567409	0.0
20	0.0	0.0	0.0	0.00600080010668089	0.0
21	0.0	0.0	0.0	0.008334444592612348	0.0
22	0.0	0.0	0.0	0.015002000266702228	0.0
23	0.0	0.0	0.0	0.021002800373383118	0.0
24	0.0	0.0	0.0	0.03600480064008534	0.0
25	0.0	0.0	0.0	0.04167222296306174	0.0
26	0.0	0.0	0.0	0.05000666755567409	0.0
27	0.0	0.0	0.0	0.10368049073209762	0.0
28	0.0	0.0	0.0	0.15352046939591946	0.0
29	0.0	0.0	0.0	0.20502733697826378	0.0
30	0.0	0.0	0.0	0.31087478330444057	0.0
31	0.0	0.0	0.0	0.4567275636751567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGCG	35	2.3864186E-5	31.714287	7
GGTATCA	3020	0.0	31.67053	1
GTCGCGT	35	8.8644313E-4	26.428572	8
TATTGTG	35	8.8644313E-4	26.428572	5
CGCGTAA	35	8.8644313E-4	26.428572	10
GCGTAAC	35	8.8644313E-4	26.428572	11
CGTAACT	40	0.0019301164	23.125002	12
GTATCAA	4275	0.0	23.022224	2
ATACCGT	60	3.7224647E-5	21.583334	6
CGGGGGT	45	0.003823709	20.555555	3
TCGCGTA	45	0.003823709	20.555555	9
GTATTGG	100	1.2867531E-8	20.349998	1
GTTGACT	55	5.1391555E-4	20.181818	4
TAGTCCT	85	1.2432774E-6	19.588236	4
ATTGGAC	115	3.0413503E-9	19.304348	3
TCGTTCG	50	0.007031059	18.499998	37
AAGCGGG	50	0.007031059	18.499998	25
TCAGTAC	50	0.007031059	18.499998	3
GTGGTAT	320	0.0	17.921875	1
TATGAGT	65	0.0015787437	17.076923	4
>>END_MODULE