FastQCFastQC Report
Wed 25 May 2016
SRR2088221_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088221_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764167
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT262261.4865939562411041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT232911.3202264864947593No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT195671.1091353596343203No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG126440.7167121933467749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90330.5120263557815105No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT78810.4467264153563693No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA77990.4420783293191631No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT73420.41617375225814796No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69290.3927632701439263No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA56290.3190741012613885No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG55560.3149361710087537No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT49840.2825129367004371No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT49520.2806990494664054No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45750.25932919049046943No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37890.21477558530456584No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA35910.2035521580439947No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.18008499195370958No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT31210.17691068929415413No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28030.15888518490596412No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC27660.15678787779161496No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27580.15633440598310705No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26590.15072269235282149No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24590.13938589714012337No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24310.13779874581034562No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG24270.13757200990609167No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22070.12510153517212372No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21950.12442132745936184No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20440.11586204707377476No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19840.11246100850996534No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA19600.11110059308444153No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA18450.10458193583714012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC18290.10367499222012429No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC18270.1035616242679973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.030.5785121
ATACGTC450.003826607320.55555529
ACGGACC951.6773811E-719.4736848
CGTCTGT4050.019.18518434
GTATTGG6300.018.51
GTCGGGT500.007036339518.49999832
GTATCAA92950.018.4104352
ATTGGAC6900.017.9637683
TTGGACC10350.017.6956524
GGACTAG1156.410664E-817.6956521
CGAACAC953.6086003E-617.52631632
CTAGGAC1401.87174E-917.1785723
ACTGCGT650.001580387217.0769238
GTAGCGA650.001580387217.07692317
GTGTTAC1201.042099E-716.9583341
TTATGCG1201.042099E-716.9583344
CGGACCA1107.8134144E-716.8181829
TATTGGA7950.016.5220132
TACCGTC1251.659082E-716.2799997
TGGACCC11650.016.1974245
Produced by FastQC (version 0.11.5)