##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088221_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1764167
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.811250295465225	31.0	30.0	31.0	26.0	34.0
2	30.5724758483749	31.0	30.0	33.0	27.0	34.0
3	30.538457526980157	31.0	30.0	34.0	27.0	34.0
4	34.697166424720564	35.0	35.0	37.0	32.0	37.0
5	34.57711089709761	35.0	35.0	37.0	30.0	37.0
6	34.70788026303632	35.0	35.0	37.0	31.0	37.0
7	34.28508128765587	35.0	35.0	37.0	30.0	37.0
8	34.549384497045914	35.0	35.0	37.0	30.0	37.0
9	35.901708284986626	37.0	35.0	39.0	30.0	39.0
10	35.80203121359826	37.0	35.0	39.0	30.0	39.0
11	35.932349941927264	37.0	35.0	39.0	30.0	39.0
12	35.65784815156388	37.0	35.0	39.0	30.0	39.0
13	35.83410810881283	37.0	35.0	39.0	30.0	39.0
14	36.6444389901863	38.0	35.0	40.0	30.0	41.0
15	36.69789084593465	38.0	35.0	40.0	31.0	41.0
16	36.60081726956688	38.0	35.0	40.0	30.0	41.0
17	36.713218759901984	38.0	36.0	40.0	31.0	41.0
18	36.714936284376705	38.0	36.0	40.0	31.0	41.0
19	36.81574590160682	39.0	36.0	40.0	31.0	41.0
20	36.77701544128192	39.0	35.0	40.0	30.0	41.0
21	36.72923991889657	38.0	35.0	40.0	30.0	41.0
22	36.63210058911656	38.0	35.0	40.0	30.0	41.0
23	36.47839518594328	38.0	35.0	40.0	30.0	41.0
24	36.40082373154016	38.0	35.0	40.0	30.0	41.0
25	36.30632474136519	38.0	35.0	40.0	30.0	41.0
26	36.02478960325185	38.0	34.0	40.0	29.0	41.0
27	35.85107362284863	38.0	34.0	40.0	29.0	41.0
28	35.664176917491375	38.0	34.0	40.0	27.0	41.0
29	35.47744856354302	38.0	34.0	40.0	27.0	41.0
30	35.29772068063851	38.0	34.0	40.0	27.0	41.0
31	35.025586013115536	37.0	33.0	40.0	26.0	41.0
32	34.78635185897934	37.0	33.0	40.0	25.0	41.0
33	34.9047369098277	37.0	33.0	40.0	26.0	41.0
34	34.86200172659391	37.0	33.0	40.0	25.0	41.0
35	34.75263849737581	37.0	33.0	40.0	25.0	41.0
36	34.64884163460715	37.0	33.0	40.0	25.0	41.0
37	34.46870676075451	37.0	33.0	40.0	24.0	41.0
38	34.33615808480717	37.0	33.0	40.0	24.0	41.0
39	34.257670617350854	37.0	33.0	40.0	23.0	41.0
40	34.08597712121358	37.0	33.0	40.0	23.0	41.0
41	33.9086651093689	37.0	33.0	40.0	21.0	41.0
42	33.67753959800858	37.0	32.0	40.0	20.0	41.0
43	33.31482903829399	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	1.0
15	9.0
16	31.0
17	98.0
18	246.0
19	557.0
20	1135.0
21	2086.0
22	3925.0
23	6526.0
24	10431.0
25	15692.0
26	23332.0
27	32220.0
28	43446.0
29	56678.0
30	70791.0
31	85128.0
32	101357.0
33	119852.0
34	140324.0
35	163599.0
36	196617.0
37	238809.0
38	266978.0
39	184297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00277014591022	25.95105792138726	13.971693156033412	21.074478776669103
2	19.006137174088394	23.948809834896583	34.60358344759878	22.441469543416243
3	21.154913338703196	26.709772940997084	32.07139686889053	20.063916851409193
4	13.966364862283445	16.572920817586997	34.69824568762481	34.76246863250474
5	10.858665874602574	41.53144231810254	34.416242906708945	13.193648900585941
6	31.824368101205835	36.149582210754424	14.839298093661203	17.186751594378535
7	23.801034709299064	35.913266714545735	21.447799443023253	18.837899133131955
8	27.937094390723782	33.93488258197778	20.14826260779167	17.97976041950677
9	24.833079861487036	13.50246320217984	24.00787453795474	37.65658239837838
10	16.309793800700273	28.277878454817483	33.68428272380109	21.72804502068115
11	31.208723437180268	21.463444220416775	25.24432210782766	22.0835102345753
12	19.640544234190983	27.733768968584037	32.10126932427599	20.52441747294899
13	31.072908630532144	20.62395453491648	29.741515400752878	18.5616214337985
14	20.570501545488607	22.6379362044523	29.00921511398864	27.782347136070452
15	23.11385486748137	28.827996442513665	27.561959837135603	20.49618885286937
16	21.397747492159187	28.400485895042817	27.896508663862324	22.305257948935672
17	18.909831098756523	27.07912572902679	28.72987647994776	25.28116669226893
18	20.037785538443924	25.93399604459215	33.40517082566446	20.62304759129946
19	19.266259940243753	24.52687302279206	34.8656334689403	21.341233568023892
20	24.059627008100705	23.307260593809996	33.60118401489201	19.031928383197283
21	23.42402958450079	24.12350984912426	32.241675532985255	20.210785033389698
22	21.52914094867436	23.777340807304522	33.2338718500006	21.45964639402052
23	20.521923378002196	26.161242104630684	34.34119332240088	18.975641194966236
24	19.341876364312448	24.800599943202656	32.699285271745815	23.15823842073908
25	20.26758237740531	26.233967645920142	33.711604400263695	19.78684557641085
26	21.917822972541714	24.895602287085065	32.242639160578335	20.943935579794886
27	20.745768399476923	26.543688891131055	31.82159058637873	20.888952123013297
28	20.322509150210834	25.93399604459215	32.203867320950906	21.539627484246108
29	19.41482864150616	25.646098130165683	33.17503388284669	21.764039345481464
30	18.664672902281925	25.90769467969869	35.11674348290156	20.31088893511782
31	20.121451087113634	25.3589937914041	33.83687598736401	20.682679134118256
32	21.262556209247762	25.9283276469858	31.560844296486675	21.248271847279764
33	22.37191830478634	25.742347521521484	31.065766449548143	20.81996772414403
34	20.514894564970323	28.72454818619779	31.141609609521094	19.61894763931079
35	19.138324206268454	29.713570200553573	31.181458444693728	19.966647148484242
36	20.321035366833186	25.689404687878188	33.55079196017157	20.438767985117053
37	20.280449639971724	26.277047467728394	32.59334292048315	20.84915997181673
38	20.760279497349174	25.63782226966041	33.00141086416422	20.600487368826194
39	21.705258062303624	25.24857340603242	32.83062204428492	20.21554648737903
40	19.93490412188869	26.22586183734306	31.601486707324195	22.237747333444055
41	21.07975038644301	25.800221861082314	31.436422969027312	21.68360478344737
42	18.585201967840913	27.603509191590135	31.667240119557842	22.144048721011107
43	19.393345414578096	27.348487983280496	31.225048422286548	22.033118179854856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	187.5
2	234.0
3	587.0
4	940.0
5	940.0
6	1867.0
7	2794.0
8	3241.0
9	3688.0
10	5818.5
11	7949.0
12	7949.0
13	15398.0
14	22847.0
15	37236.0
16	51625.0
17	49237.0
18	46849.0
19	46849.0
20	51339.5
21	55830.0
22	44696.0
23	33562.0
24	35627.0
25	37692.0
26	37692.0
27	40814.0
28	43936.0
29	45881.0
30	47826.0
31	50838.5
32	53851.0
33	53851.0
34	58057.0
35	62263.0
36	68400.0
37	74537.0
38	76949.0
39	79361.0
40	79361.0
41	83675.5
42	87990.0
43	101448.0
44	114906.0
45	146856.5
46	178807.0
47	178807.0
48	181796.5
49	184786.0
50	167053.5
51	149321.0
52	139423.5
53	129526.0
54	129526.0
55	110502.5
56	91479.0
57	74990.0
58	58501.0
59	50773.5
60	43046.0
61	43046.0
62	37521.5
63	31997.0
64	27779.0
65	23561.0
66	19925.5
67	16290.0
68	16290.0
69	13622.5
70	10955.0
71	9234.5
72	7514.0
73	5903.5
74	4293.0
75	4293.0
76	3286.5
77	2280.0
78	1809.5
79	1339.0
80	1058.5
81	778.0
82	778.0
83	587.5
84	397.0
85	306.0
86	215.0
87	183.0
88	151.0
89	151.0
90	119.0
91	87.0
92	51.5
93	16.0
94	11.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1764167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.09007140044527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76277835476824	35.840920066682685
2	12.591665475521568	11.607015216346213
3	4.079749662626528	5.641078597391975
4	1.7989344514767251	3.316520692531324
5	0.9244277581362601	2.130347068852689
6	0.553591108806922	1.530903223893762
7	0.36457295818833163	1.1762235571500226
8	0.242094008564468	0.892650411228507
9	0.1924512176651577	0.7983081326960776
>10	1.278965370238792	11.437163276282208
>50	0.1259975682412346	3.9799981668848723
>100	0.07022925602401618	6.045403402613523
>500	0.006339173477216114	2.0281386403244577
>1k	0.006836363553860515	6.019106671496793
>5k	8.70082634127702E-4	2.874122576307489
>10k+	4.971900766444011E-4	4.682100299317476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26226	1.4865939562411041	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23291	1.3202264864947593	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19567	1.1091353596343203	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12644	0.7167121933467749	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9033	0.5120263557815105	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7881	0.4467264153563693	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7799	0.4420783293191631	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7342	0.41617375225814796	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6929	0.3927632701439263	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5629	0.3190741012613885	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5556	0.3149361710087537	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4984	0.2825129367004371	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4952	0.2806990494664054	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4575	0.25932919049046943	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3789	0.21477558530456584	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3591	0.2035521580439947	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.18008499195370958	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3121	0.17691068929415413	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2803	0.15888518490596412	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2766	0.15678787779161496	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2758	0.15633440598310705	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2659	0.15072269235282149	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2459	0.13938589714012337	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2431	0.13779874581034562	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2427	0.13757200990609167	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2207	0.12510153517212372	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2195	0.12442132745936184	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2044	0.11586204707377476	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1984	0.11246100850996534	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1960	0.11110059308444153	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1845	0.10458193583714012	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1829	0.10367499222012429	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1827	0.1035616242679973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.668397606349059E-5	0.0
3	0.0	0.0	0.0	1.1336795212698118E-4	0.0
4	0.0	0.0	0.0	1.1336795212698118E-4	0.0
5	5.668397606349059E-5	0.0	0.0	1.1336795212698118E-4	0.0
6	5.668397606349059E-5	0.0	0.0	1.1336795212698118E-4	0.0
7	5.668397606349059E-5	0.0	0.0	1.1336795212698118E-4	0.0
8	2.2673590425396236E-4	0.0	0.0	1.1336795212698118E-4	0.0
9	2.2673590425396236E-4	0.0	0.0	2.834198803174529E-4	0.0
10	2.2673590425396236E-4	0.0	0.0	3.401038563809435E-4	0.0
11	2.2673590425396236E-4	0.0	0.0	5.668397606349058E-4	0.0
12	2.2673590425396236E-4	0.0	0.0	6.80207712761887E-4	5.668397606349059E-5
13	3.401038563809435E-4	0.0	0.0	9.069436170158494E-4	5.668397606349059E-5
14	3.401038563809435E-4	0.0	0.0	9.6362759307934E-4	5.668397606349059E-5
15	3.401038563809435E-4	0.0	0.0	0.001643835305841227	5.668397606349059E-5
16	3.401038563809435E-4	0.0	0.0	0.002494094946793586	5.668397606349059E-5
17	4.534718085079247E-4	0.0	0.0	0.0037978263962538694	5.668397606349059E-5
18	4.534718085079247E-4	0.0	0.0	0.004364666156888775	5.668397606349059E-5
19	6.80207712761887E-4	0.0	0.0	0.005214925797841134	5.668397606349059E-5
20	6.80207712761887E-4	0.0	0.0	0.006632025199428399	5.668397606349059E-5
21	7.935756648888683E-4	0.0	0.0	0.00957959195472991	5.668397606349059E-5
22	7.935756648888683E-4	0.0	0.0	0.01632498510628529	5.668397606349059E-5
23	8.502596409523588E-4	0.0	0.0	0.027888516223237368	5.668397606349059E-5
24	8.502596409523588E-4	0.0	0.0	0.045063760970475014	5.668397606349059E-5
25	8.502596409523588E-4	0.0	0.0	0.051525734241712945	5.668397606349059E-5
26	8.502596409523588E-4	0.0	0.0	0.05872459920177625	5.668397606349059E-5
27	8.502596409523588E-4	0.0	0.0	0.10974017765891778	5.668397606349059E-5
28	8.502596409523588E-4	0.0	0.0	0.15729803357618638	5.668397606349059E-5
29	8.502596409523588E-4	0.0	0.0	0.21386864168755	5.668397606349059E-5
30	8.502596409523588E-4	0.0	0.0	0.3227018757294519	1.1336795212698118E-4
31	8.502596409523588E-4	0.0	0.0	0.4709871571115433	1.1336795212698118E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5445	0.0	30.578512	1
ATACGTC	45	0.0038266073	20.555555	29
ACGGACC	95	1.6773811E-7	19.473684	8
CGTCTGT	405	0.0	19.185184	34
GTATTGG	630	0.0	18.5	1
GTCGGGT	50	0.0070363395	18.499998	32
GTATCAA	9295	0.0	18.410435	2
ATTGGAC	690	0.0	17.963768	3
TTGGACC	1035	0.0	17.695652	4
GGACTAG	115	6.410664E-8	17.695652	1
CGAACAC	95	3.6086003E-6	17.526316	32
CTAGGAC	140	1.87174E-9	17.178572	3
ACTGCGT	65	0.0015803872	17.076923	8
GTAGCGA	65	0.0015803872	17.076923	17
GTGTTAC	120	1.042099E-7	16.958334	1
TTATGCG	120	1.042099E-7	16.958334	4
CGGACCA	110	7.8134144E-7	16.818182	9
TATTGGA	795	0.0	16.522013	2
TACCGTC	125	1.659082E-7	16.279999	7
TGGACCC	1165	0.0	16.197424	5
>>END_MODULE