FastQCFastQC Report
Wed 25 May 2016
SRR2088220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259395
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT346981.5357208456246032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306431.356248022147522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276641.2243985668729904No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG190030.8410658605511653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119540.5290796872614129No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT113830.5038074351762307No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT107260.4747288544057148No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107080.4739321809599472No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA104940.46446061888248846No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA80200.3549622797253247No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG75760.3353110013963915No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74330.328981873466127No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT68920.30503741045722416No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58800.26024665895073684No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58090.25710422480354256No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56730.2510849143244098No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT45210.20009781379528593No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42300.18721825975537698No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41030.18159728599912808No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39950.1768172453245227No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC39010.172656839552181No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37780.167212904339436No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37740.1670358657959321No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34340.15198758959810038No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34090.15088109870120098No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC30770.13618689959037708No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.12441383644736755No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.12317456664284022No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA27600.1221565950176928No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA27560.1219795564741889No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC26490.1172437754354595No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA25910.1146767165546529No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG24730.10945407952128777No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG24700.10932130061365986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC23460.10383310576503887No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG23230.10281513413989143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74900.030.4546051
GTATTGG8700.021.2643661
ACGTATA450.003826934620.55555529
ATTGGAC9100.019.5164833
TATTGGA9650.018.7875652
ACGACGC902.154089E-618.527
GTATCAA128300.018.4134852
AGCCGTC1451.546141E-1017.8620712
AGTATCA4250.017.847061
TTGGACC15850.017.7413234
ACGTTTA1301.3951649E-817.07692126
GTGTCGC650.001580573217.07692111
GGACCCT16100.017.0062126
CGATCTG1757.2759576E-1216.91428633
GTGCGAA1107.8158155E-716.81818212
CGTCTGT5550.016.33333434
TGGACCC16700.016.0628745
TATACCG1403.477726E-815.8571435
GTTATAC1701.4879333E-915.2352933
ACGCTGC1353.9767838E-715.07407330
Produced by FastQC (version 0.11.5)