FastQCFastQC Report
Wed 25 May 2016
SRR2088219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences891539
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188332.112414599922157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT152431.7097401235391834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102751.1525014609568398No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65100.7301980059201No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT50640.5680065594438382No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT50360.5648659228592355No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG43220.4847796899518697No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34230.3839428224676655No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32480.3643138438138993No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31660.3551162652447061No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30060.33716977047554847No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26910.3018376088987694No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT26400.2961171636911004No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT24080.2700947462758219No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.25035360202974855No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC15370.17239851537622022No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.17183768741468403No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15100.1693700443839249No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14970.16791189168393084No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.16106979055318948No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA13690.15355469586860473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC13350.14974106573015875No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA13320.14940456895323706No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT13230.14839507862247195No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12870.14435711729941147No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12100.13572036669175438No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA11390.12775660963794067No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG11360.12742011286101898No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11200.12562546338410321No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG11090.12439164186872365No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11020.12360648272257298No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11010.12349431713026575No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC10670.11968068699181977No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA10210.11452106974568696No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG9840.11037094283031926No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.10924928690724689No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC9270.10397750406880685No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC9190.10308017933034898No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG9010.10106119866881876No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC9010.10106119866881876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC8950.10038820511497534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.030.4651741
TACGTTT358.867827E-426.4285724
CGTCTGT2300.023.32608834
GTACGTT400.001930848923.1250023
TGCGTCT2400.021.58333432
GTATCAA60550.020.8067722
GTATGCA450.003825144420.5555579
CCTATAG450.003825144420.5555573
CGACCGC656.8999114E-519.92307736
GGACTGC951.6750528E-719.4736846
ACGTTTT500.007033673618.55
CGTTGGG500.007033673618.512
GCTGCGT701.21880774E-418.58
AGTATAC500.007033673618.52
TATACCG609.234599E-418.531
CGATCTG701.21880774E-418.533
AGTACGT500.007033673618.52
TTGGACC3850.017.779224
GCGTCTG3100.017.30645233
TAAACGT650.001579558417.0769234
Produced by FastQC (version 0.11.5)