##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088219_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	891539
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.52559114071286	31.0	30.0	31.0	26.0	34.0
2	30.207817044459077	31.0	30.0	31.0	27.0	34.0
3	30.092426691373007	31.0	30.0	31.0	26.0	34.0
4	34.20091437390849	35.0	35.0	37.0	30.0	37.0
5	33.99790699004755	35.0	33.0	37.0	30.0	37.0
6	34.285253926076145	35.0	35.0	37.0	30.0	37.0
7	33.85266712953668	35.0	33.0	37.0	29.0	37.0
8	34.17648358624805	35.0	33.0	37.0	30.0	37.0
9	35.40737084973288	37.0	34.0	39.0	30.0	39.0
10	35.27059836978528	37.0	34.0	39.0	30.0	39.0
11	35.50136449443042	37.0	35.0	39.0	30.0	39.0
12	35.15941310475481	37.0	34.0	39.0	29.0	39.0
13	35.39319648383301	37.0	34.0	39.0	30.0	39.0
14	36.07638925498492	38.0	34.0	40.0	29.0	41.0
15	36.17644993657036	38.0	34.0	40.0	30.0	41.0
16	35.98436860305606	38.0	34.0	40.0	29.0	41.0
17	36.15544468609898	38.0	34.0	40.0	30.0	41.0
18	36.163218883301795	38.0	34.0	40.0	30.0	41.0
19	36.275892585742184	38.0	34.0	40.0	30.0	41.0
20	36.25507801677773	38.0	34.0	40.0	30.0	41.0
21	36.22855758413261	38.0	34.0	40.0	29.0	41.0
22	36.127744271422785	38.0	34.0	40.0	29.0	41.0
23	35.9360813155678	38.0	34.0	40.0	29.0	41.0
24	35.84030311629665	38.0	34.0	40.0	28.0	41.0
25	35.72837307173326	38.0	34.0	40.0	27.0	41.0
26	35.414252208820926	38.0	34.0	40.0	27.0	41.0
27	35.18857728041061	37.0	33.0	40.0	27.0	41.0
28	34.97729095418148	37.0	33.0	40.0	26.0	41.0
29	34.76445562112258	37.0	33.0	40.0	26.0	41.0
30	34.52957862751938	37.0	33.0	39.0	25.0	40.0
31	34.23969674910464	36.0	33.0	39.0	25.0	40.0
32	33.98719293267036	36.0	32.0	39.0	24.0	40.0
33	34.07600228369146	36.0	33.0	39.0	24.0	40.0
34	33.986505357589515	36.0	33.0	39.0	24.0	40.0
35	33.828479741211545	36.0	32.0	39.0	24.0	40.0
36	33.68094272937022	36.0	32.0	39.0	23.0	40.0
37	33.46582370485195	36.0	32.0	39.0	22.0	40.0
38	33.29430905434311	36.0	31.0	39.0	21.0	40.0
39	33.14988127272054	36.0	31.0	39.0	20.0	40.0
40	32.92429832009592	36.0	31.0	39.0	18.0	40.0
41	32.69734582558923	36.0	31.0	39.0	16.0	40.0
42	32.436207501859144	36.0	30.0	39.0	15.0	40.0
43	32.01533752309209	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	5.0
15	19.0
16	42.0
17	95.0
18	226.0
19	504.0
20	980.0
21	1832.0
22	3070.0
23	4931.0
24	7542.0
25	10836.0
26	15423.0
27	20722.0
28	26939.0
29	34648.0
30	41708.0
31	48856.0
32	57718.0
33	67213.0
34	77572.0
35	89146.0
36	106035.0
37	120109.0
38	111040.0
39	44326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.61547279479641	27.512761640264756	14.69716972560931	19.17459583932952
2	22.19319625950183	25.359406599150457	30.81749648641282	21.629900654934893
3	24.963013395936688	25.53191728011899	31.3070992968339	18.197970027110426
4	17.127798110907094	18.423871530017195	31.77696096300891	32.6713693960668
5	10.698802856633305	43.11320088072423	33.35580384032555	12.832192422316915
6	32.91869452710425	35.79338649234638	13.396497517214614	17.89142146333475
7	22.452635274508463	37.312781605740184	21.431591887735703	18.80299123201565
8	28.20762748460808	35.0712644090724	19.620454068750778	17.100654037568745
9	24.12838922357855	13.342209370537914	24.674635658114788	37.854765747768745
10	17.268565929252674	29.7598871165479	32.76637365275103	20.205173301448394
11	31.59558920024811	21.141419500436882	24.185481510062935	23.07750978925207
12	18.600308006716475	29.183804634457942	32.15002372302278	20.06586363580281
13	31.308445283941587	20.252507181402045	30.939420485250785	17.49962704940558
14	20.33517322293248	22.982729863752454	30.378256026937688	26.303840886377376
15	24.13388533760161	28.623537500883305	27.88503924113247	19.35753792038262
16	21.024206456475824	27.293253576119497	29.930378816854898	21.752161150549778
17	18.400989749186518	27.6281800347489	30.348980807345498	23.621849408719083
18	19.187046220075622	24.609916111353513	35.12633771489526	21.07669995367561
19	18.782016266254196	24.056378913317307	37.44031388419351	19.721290936234983
20	22.84779465620685	22.645560093276906	36.463015078420575	18.043630172095668
21	22.89086624365283	22.705568685161275	34.788831447642785	19.61473362354311
22	21.063015751414127	22.737311547784227	35.43770939914014	20.76196330166151
23	19.922403843241852	25.70465229227213	36.280072997367476	18.092870867118542
24	18.605579789554916	24.18278953584756	35.09201504364924	22.11961563094828
25	19.450411030813008	25.337534308650543	36.2758107048598	18.936243955676645
26	20.755682028492302	24.01252216672518	34.81945265434266	20.412343150439856
27	20.8532660937996	25.138552547897515	34.22037622582972	19.787805132473174
28	19.82156697575765	25.634548797080104	34.67890916718169	19.86497505998055
29	18.835182757007825	24.711650303576175	35.43423226577861	21.018934673637386
30	18.172844934433602	25.304781955696836	37.136345129040905	19.386027980828658
31	19.479910581589813	24.300226910993235	35.58520715302415	20.634655354392798
32	20.23489718340981	25.831399411579305	33.735372204693235	20.198331200317654
33	21.54364531445063	25.463384103219266	32.85487230508144	20.138098277248666
34	20.34807226604781	27.711070407463943	33.228383727464525	18.71247359902371
35	18.582585843131934	28.47031930179162	33.40818517193303	19.53890968314342
36	19.94730460473406	24.923194610667622	35.50646690722447	19.623033877373842
37	19.32545856098275	25.712503883733635	34.75260196132754	20.20943559395607
38	20.474370722985757	25.384643857419587	34.11572572820707	20.02525969138759
39	21.502817038850797	25.19519617201267	33.69981571193184	19.6021710772047
40	20.049038796956722	26.004246589324755	32.61068781062858	21.33602680308994
41	20.28492303757884	26.00121811833246	32.44019611032159	21.273662733767114
42	18.399419430894216	27.32140713978861	32.1566414929689	22.12253193634827
43	19.16427660483725	26.874539420036587	32.062534561023135	21.898649414103026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	81.0
2	108.0
3	234.0
4	360.0
5	360.0
6	942.5
7	1525.0
8	1818.0
9	2111.0
10	3452.5
11	4794.0
12	4794.0
13	9882.0
14	14970.0
15	24398.5
16	33827.0
17	30593.5
18	27360.0
19	27360.0
20	31773.0
21	36186.0
22	27672.5
23	19159.0
24	19466.0
25	19773.0
26	19773.0
27	20737.0
28	21701.0
29	22361.0
30	23021.0
31	24321.5
32	25622.0
33	25622.0
34	27709.0
35	29796.0
36	32889.0
37	35982.0
38	37080.0
39	38178.0
40	38178.0
41	40801.0
42	43424.0
43	50406.5
44	57389.0
45	72675.0
46	87961.0
47	87961.0
48	90690.0
49	93419.0
50	83549.0
51	73679.0
52	67395.5
53	61112.0
54	61112.0
55	51780.0
56	42448.0
57	35153.0
58	27858.0
59	24086.5
60	20315.0
61	20315.0
62	17944.5
63	15574.0
64	13519.5
65	11465.0
66	9691.5
67	7918.0
68	7918.0
69	6680.5
70	5443.0
71	4565.0
72	3687.0
73	3003.0
74	2319.0
75	2319.0
76	1835.5
77	1352.0
78	1059.0
79	766.0
80	598.0
81	430.0
82	430.0
83	326.0
84	222.0
85	167.5
86	113.0
87	88.5
88	64.0
89	64.0
90	51.0
91	38.0
92	24.0
93	10.0
94	7.0
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	891539.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.592687004249214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.99464040118336	41.48329177843037
2	10.2756677807031	10.397472875775202
3	3.1529067908112856	4.785420792632616
4	1.3234151657438409	2.6782051702861893
5	0.7456561604377459	1.8862374368908554
6	0.4505212052825757	1.3675846996583074
7	0.33277836987238135	1.1785306336116363
8	0.2321852794676135	0.9397501736879279
9	0.18225264857274995	0.8298586084442909
>10	1.1586237542238451	10.86081683630348
>50	0.08149789420952502	2.8021823140341127
>100	0.05507762556402385	5.530572625183439
>500	0.007164569177175871	2.544006770541854
>1k	0.006268998030028889	5.81081095872597
>5k	6.71678360360238E-4	1.8814707893806577
>10k+	6.71678360360238E-4	5.0237875364130975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18833	2.112414599922157	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15243	1.7097401235391834	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10275	1.1525014609568398	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6510	0.7301980059201	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5064	0.5680065594438382	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5036	0.5648659228592355	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4322	0.4847796899518697	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3423	0.3839428224676655	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3248	0.3643138438138993	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3166	0.3551162652447061	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3006	0.33716977047554847	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2691	0.3018376088987694	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2640	0.2961171636911004	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2408	0.2700947462758219	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2232	0.25035360202974855	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1537	0.17239851537622022	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1532	0.17183768741468403	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1510	0.1693700443839249	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1497	0.16791189168393084	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1436	0.16106979055318948	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1369	0.15355469586860473	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1335	0.14974106573015875	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1332	0.14940456895323706	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1323	0.14839507862247195	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1287	0.14435711729941147	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1210	0.13572036669175438	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1139	0.12775660963794067	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1136	0.12742011286101898	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1120	0.12562546338410321	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1109	0.12439164186872365	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1102	0.12360648272257298	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1101	0.12349431713026575	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1067	0.11968068699181977	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1021	0.11452106974568696	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	984	0.11037094283031926	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	974	0.10924928690724689	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	927	0.10397750406880685	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	919	0.10308017933034898	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	901	0.10106119866881876	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	901	0.10106119866881876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	895	0.10038820511497534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.1216559230723502E-4	0.0	0.0	0.0	0.0
11	2.2433118461447004E-4	0.0	0.0	2.2433118461447004E-4	0.0
12	2.2433118461447004E-4	0.0	0.0	5.608279615361751E-4	0.0
13	2.2433118461447004E-4	0.0	0.0	6.729935538434101E-4	0.0
14	2.2433118461447004E-4	0.0	0.0	0.0011216559230723502	0.0
15	2.2433118461447004E-4	0.0	0.0	0.0015703182923012902	0.0
16	2.2433118461447004E-4	0.0	0.0	0.0021311462538374654	0.0
17	2.2433118461447004E-4	0.0	0.0	0.0032528021769098153	0.0
18	2.2433118461447004E-4	0.0	0.0	0.0037014645461387555	0.0
19	2.2433118461447004E-4	0.0	0.0	0.004598789284596636	0.0
20	2.2433118461447004E-4	0.0	0.0	0.005271782838440046	0.0
21	3.3649677692170507E-4	0.0	0.0	0.007627260276891981	0.0
22	3.3649677692170507E-4	0.0	0.0	0.012899043115332028	0.0
23	3.3649677692170507E-4	0.0	0.0	0.022096621684525298	0.0
24	3.3649677692170507E-4	0.0	0.0	0.03600515513062244	0.0
25	3.3649677692170507E-4	0.0	0.0	0.03981878526906843	0.0
26	3.3649677692170507E-4	0.0	0.0	0.04834337028441829	0.0
27	3.3649677692170507E-4	0.0	0.0	0.0893959770688663	0.0
28	3.3649677692170507E-4	0.0	0.0	0.1284296031917841	0.0
29	3.3649677692170507E-4	0.0	0.0	0.1732958401146781	0.0
30	3.3649677692170507E-4	0.0	0.0	0.2761516882604126	0.0
31	3.3649677692170507E-4	0.0	0.0	0.40054333012913623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4020	0.0	30.465174	1
TACGTTT	35	8.867827E-4	26.428572	4
CGTCTGT	230	0.0	23.326088	34
GTACGTT	40	0.0019308489	23.125002	3
TGCGTCT	240	0.0	21.583334	32
GTATCAA	6055	0.0	20.806772	2
GTATGCA	45	0.0038251444	20.555557	9
CCTATAG	45	0.0038251444	20.555557	3
CGACCGC	65	6.8999114E-5	19.923077	36
GGACTGC	95	1.6750528E-7	19.473684	6
ACGTTTT	50	0.0070336736	18.5	5
CGTTGGG	50	0.0070336736	18.5	12
GCTGCGT	70	1.21880774E-4	18.5	8
AGTATAC	50	0.0070336736	18.5	2
TATACCG	60	9.234599E-4	18.5	31
CGATCTG	70	1.21880774E-4	18.5	33
AGTACGT	50	0.0070336736	18.5	2
TTGGACC	385	0.0	17.77922	4
GCGTCTG	310	0.0	17.306452	33
TAAACGT	65	0.0015795584	17.076923	4
>>END_MODULE