FastQCFastQC Report
Wed 25 May 2016
SRR2088218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861241
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT253801.3636063250272266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT243971.3107921005393712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT202731.0892195046208417No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG105750.5681693020946778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86500.4647436844556938No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT71110.38205691793808544No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA67430.3622851635011264No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT56470.30339972093887896No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55340.29732850286448664No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA49620.2665963193374743No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT48980.26315775334843794No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG45460.24424564040873803No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT41480.22286205816441826No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37310.20045765164210333No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35760.1921298746374059No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT34770.1868108428731153No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34350.1845542839428102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32030.17208948223255344No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28770.15457428672589954No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27090.14554805100467913No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25850.1388858294009212No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC25710.13813364309081952No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23730.1274955795622383No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA20530.11030274961705656No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20480.11003411164916312No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20020.10756264234454324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.10691791122159892No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC19210.10321070726466912No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18760.10079296555362792No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC18660.10025568961784101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55600.028.7814751
ATTGGAC5200.022.4134643
GTATTGG5950.021.1428571
TTGGACC9500.020.0578944
GGACCCT9600.019.0781256
TATTGGA6800.018.52
CTGTGCG2000.018.59
GACCCTC9800.018.122457
TGGACCC10300.017.9611645
GTATCAA94650.017.1806662
ACAGCGC650.001580430717.0769258
ATAACCG650.001580430717.07692510
ATAATAC2150.016.3488373
TTTTTAC15550.016.2990361
CGTTTAG1059.34924E-615.85714226
GTTATCA2100.015.8571421
CGTCTGT4950.015.69696934
CGGGATA1302.5912777E-715.65384824
TAGGTCG1557.215931E-915.51612921
GGTCGTC1752.240995E-914.823
Produced by FastQC (version 0.11.5)