FastQCFastQC Report
Wed 25 May 2016
SRR2088217_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088217_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804875
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT386931.0169322251059496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385431.012989914254739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT345200.9072571372252702No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG207880.5463517198331089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142040.3733105555373041No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA119910.3151483294457768No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA118090.31036499227964126No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT114180.3000887019941522No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT104530.27472650218469724No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA91520.2405335260685305No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT86200.2265514635829035No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT83590.21969184270179704No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG78600.20657708860343638No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68610.1803212983343737No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA63140.16594500476362561No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA62930.16539308124445615No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT59770.15708794638457244No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50460.13261933703472517No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48970.12870330825585596No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC46300.12168599494070108No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC42930.11282893656164789No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39450.10368277538683926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85600.029.2844621
GTATTGG10900.023.5917451
ATTGGAC10450.023.0143553
TTGGACC18350.020.2643054
TAGTGCG1303.274181E-1119.9230777
GGACCCT19550.018.5473146
TGGACCC19750.018.5468375
TATTGGA14950.017.2006682
CGTCTGT9500.016.94210634
GTATCAA153100.016.6632942
TACGGGT803.3851352E-416.18754
GACCCTC21050.015.9952497
ACGTTTA2450.015.85714226
CCGTCGA957.066533E-515.5789489
TAGTACG1902.7284841E-1115.5789484
CGCGAAT855.368196E-415.23529435
TTATGCG1651.6485501E-814.5757574
CGAACGA1152.2123879E-514.47826116
TCTATAC3750.014.3066673
CGCGATA1051.6572184E-414.09523814
Produced by FastQC (version 0.11.5)