##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088217_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3804875
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.477265087552155	31.0	31.0	33.0	27.0	34.0
2	30.87523322053944	31.0	31.0	34.0	27.0	34.0
3	31.079195768586352	31.0	31.0	34.0	28.0	34.0
4	35.12118137915174	35.0	35.0	37.0	32.0	37.0
5	34.951877262722164	35.0	35.0	37.0	32.0	37.0
6	34.9813476132593	36.0	35.0	37.0	32.0	37.0
7	34.80144498833733	35.0	35.0	37.0	32.0	37.0
8	34.90029449062059	36.0	35.0	37.0	32.0	37.0
9	36.40092933407799	38.0	35.0	39.0	32.0	39.0
10	36.256687539012454	38.0	35.0	39.0	32.0	39.0
11	36.291620881106475	38.0	35.0	39.0	32.0	39.0
12	36.119073556949964	38.0	35.0	39.0	31.0	39.0
13	36.186025822136074	38.0	35.0	39.0	32.0	39.0
14	37.08461513190315	39.0	36.0	40.0	31.0	41.0
15	37.15508945760373	39.0	36.0	40.0	31.0	41.0
16	37.13269555504451	39.0	36.0	40.0	31.0	41.0
17	37.20278537402674	39.0	36.0	40.0	31.0	41.0
18	37.2021540786491	39.0	36.0	40.0	31.0	41.0
19	37.30102644633529	39.0	36.0	40.0	31.0	41.0
20	37.26131975426262	39.0	36.0	40.0	31.0	41.0
21	37.15010847925359	39.0	36.0	40.0	31.0	41.0
22	37.098474719931666	39.0	36.0	40.0	31.0	41.0
23	36.9763188015375	39.0	36.0	40.0	31.0	41.0
24	36.91071980025625	39.0	35.0	40.0	31.0	41.0
25	36.81328663885147	39.0	35.0	40.0	30.0	41.0
26	36.55242103879891	38.0	35.0	40.0	30.0	41.0
27	36.41053043792503	38.0	35.0	40.0	30.0	41.0
28	36.26842537534085	38.0	35.0	40.0	30.0	41.0
29	36.152165839876474	38.0	35.0	40.0	30.0	41.0
30	36.00166680902789	38.0	35.0	40.0	29.0	41.0
31	35.76521856828411	38.0	34.0	40.0	29.0	41.0
32	35.46181464568481	38.0	34.0	40.0	27.0	41.0
33	35.63770320969808	38.0	34.0	40.0	28.0	41.0
34	35.623175005749204	38.0	34.0	40.0	28.0	41.0
35	35.55958027530471	38.0	34.0	40.0	27.0	41.0
36	35.47012608824206	38.0	34.0	40.0	27.0	41.0
37	35.351402345674956	38.0	34.0	40.0	27.0	41.0
38	35.239843096028125	38.0	34.0	40.0	26.0	41.0
39	35.21595295509051	38.0	34.0	40.0	25.0	41.0
40	35.07908722362758	38.0	34.0	40.0	25.0	41.0
41	34.963985939091295	38.0	33.0	40.0	24.0	41.0
42	34.78806320838398	38.0	33.0	40.0	24.0	41.0
43	34.45558211504977	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	7.0
15	14.0
16	34.0
17	134.0
18	280.0
19	669.0
20	1447.0
21	2810.0
22	5232.0
23	9102.0
24	15061.0
25	23788.0
26	36019.0
27	52695.0
28	73954.0
29	98535.0
30	126807.0
31	156207.0
32	189471.0
33	230866.0
34	276884.0
35	333146.0
36	409237.0
37	519482.0
38	635484.0
39	607505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11448470711916	23.575597095831004	13.569565360228655	22.740352836821184
2	17.41489536449949	23.18223331909721	36.39875160156378	23.004119714839515
3	19.708479253589147	26.25568514077335	31.953060218798253	22.082775386839252
4	13.114701534215973	16.07427313643681	36.012957061664316	34.79806826768291
5	11.553993232366372	40.16925654587864	34.96848122474457	13.308268997010414
6	31.62174841486251	36.654607575807354	15.746929925424618	15.976714083905517
7	24.832090410328856	34.698064982423865	21.974361838430962	18.49548276881632
8	27.26814941358126	33.94384835244259	20.471710634383523	18.316291599592628
9	25.33946581687966	13.779611682381157	23.444764939715494	37.436157561023684
10	15.961575610236867	27.71799336377673	34.46854364466638	21.85188738132002
11	31.852741548671116	22.059778573540523	25.110286146062617	20.977193731725745
12	20.043496829725026	27.08262426492329	32.17312001051283	20.70075889483886
13	30.52470843325996	21.137409244718945	28.508873484674268	19.829008837346827
14	20.886205197279807	22.336607641512533	28.513236308682938	28.263950852524722
15	22.778750944511973	29.25541574953185	26.645605966030423	21.320227339925754
16	21.88395150957653	28.48387923387759	27.042964617760113	22.589204638785766
17	19.597831729031835	27.25332632478071	28.120056506455533	25.02878543973192
18	20.678603107855054	26.7413778376425	31.893399914583263	20.68661913991918
19	20.075928906994317	25.290292059528895	33.07038996024836	21.56338907322842
20	23.917290318341603	24.55305364828017	31.789112651532577	19.740543381845658
21	23.37028154669996	25.218489437892178	30.649758533460364	20.7614704819475
22	21.846000197115544	24.944998193107526	31.52069384670981	21.68830776306712
23	21.098459213508985	26.64487006800486	32.27259765432504	19.98407306416111
24	20.002102565787315	25.903820756266633	30.914208745359574	23.179867932586482
25	21.114123328624462	26.652702125562598	31.918604421958673	20.314570123854267
26	22.172765202536223	25.819429021978387	30.704031012845363	21.303774762640035
27	20.899057130654754	27.160997404645354	30.466414796806728	21.47353066789316
28	20.762548046913498	26.626420053221196	30.635408521961953	21.975623377903347
29	20.03726797858011	26.53556292913696	31.438141857485462	21.989027234797465
30	19.56555734419659	26.77160222083511	32.92334176549821	20.739498669470088
31	20.731009560103814	26.244541542100595	32.041814777095176	20.982634120700418
32	21.567699333092413	26.420342323992248	30.305594796149677	21.70636354676566
33	22.376214724531028	26.395847432570058	29.91496435493939	21.312973487959525
34	20.882078911922207	28.625066526495612	30.008134301389667	20.484720260192514
35	19.86165117119485	29.337862610466836	30.202989585728833	20.59749663260948
36	20.694109530536483	26.274214001774038	31.891744144025758	21.13993232366372
37	20.687880679391572	26.49829495055685	31.195742304280692	21.618082065770885
38	20.839186569861035	26.224172936036005	31.766615197608335	21.17002529649463
39	21.637872466243962	25.689937251552287	31.757311343999472	20.91487893820428
40	20.175143730083118	26.39437563651894	31.069667203258977	22.36081343013897
41	20.936351391307202	25.852570715200894	31.029718453300042	22.18135944019186
42	18.809448405006734	27.332908439830483	31.334012286868816	22.523630868293964
43	19.437984165051414	27.051663983705115	30.962541476395415	22.54781037484806
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	421.0
1	539.5
2	658.0
3	1677.5
4	2697.0
5	2697.0
6	4482.5
7	6268.0
8	6891.0
9	7514.0
10	11315.5
11	15117.0
12	15117.0
13	26762.0
14	38407.0
15	62108.0
16	85809.0
17	85311.5
18	84814.0
19	84814.0
20	90247.5
21	95681.0
22	81048.0
23	66415.0
24	73728.0
25	81041.0
26	81041.0
27	90189.5
28	99338.0
29	105689.0
30	112040.0
31	120708.0
32	129376.0
33	129376.0
34	139978.5
35	150581.0
36	163525.5
37	176470.0
38	183162.0
39	189854.0
40	189854.0
41	199855.5
42	209857.0
43	232836.0
44	255815.0
45	307595.5
46	359376.0
47	359376.0
48	363524.5
49	367673.0
50	337048.5
51	306424.0
52	286139.5
53	265855.0
54	265855.0
55	233163.5
56	200472.0
57	168514.0
58	136556.0
59	120482.5
60	104409.0
61	104409.0
62	92166.0
63	79923.0
64	70180.0
65	60437.0
66	51374.0
67	42311.0
68	42311.0
69	35593.5
70	28876.0
71	24068.5
72	19261.0
73	15069.5
74	10878.0
75	10878.0
76	8646.5
77	6415.0
78	5002.0
79	3589.0
80	2799.5
81	2010.0
82	2010.0
83	1507.5
84	1005.0
85	781.0
86	557.0
87	479.0
88	401.0
89	401.0
90	308.0
91	215.0
92	129.5
93	44.0
94	27.5
95	11.0
96	11.0
97	6.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3804875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.66944806999662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70199378623686	28.04709070948347
2	14.452957279251585	11.466816764943008
3	5.847705445862705	6.95925742539861
4	2.9397018876611627	4.664654054953822
5	1.6061579367658778	3.185769943237346
6	0.9604449475975052	2.2860192583685914
7	0.6433303558304312	1.7864392099727413
8	0.46723724707258224	1.4828034967291186
9	0.3302132657288739	1.1789440197170002
>10	1.74939021657814	13.004252532183461
>50	0.18125603451140954	5.024340552318237
>100	0.10575469534320277	7.641117941008692
>500	0.0069619215588709905	1.8272467189556416
>1k	0.005690032039568469	4.62315274702199
>5k	6.024739806601909E-4	1.7123417411957376
>10k+	6.024739806601909E-4	5.109752884512618
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38693	1.0169322251059496	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38543	1.012989914254739	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34520	0.9072571372252702	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	20788	0.5463517198331089	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14204	0.3733105555373041	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11991	0.3151483294457768	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11809	0.31036499227964126	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11418	0.3000887019941522	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10453	0.27472650218469724	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9152	0.2405335260685305	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8620	0.2265514635829035	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8359	0.21969184270179704	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7860	0.20657708860343638	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6861	0.1803212983343737	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6314	0.16594500476362561	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6293	0.16539308124445615	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5977	0.15708794638457244	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5046	0.13261933703472517	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4897	0.12870330825585596	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4630	0.12168599494070108	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4293	0.11282893656164789	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3945	0.10368277538683926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.628207234140412E-5	0.0
4	0.0	0.0	0.0	5.256414468280824E-5	0.0
5	0.0	0.0	0.0	5.256414468280824E-5	0.0
6	0.0	0.0	0.0	5.256414468280824E-5	0.0
7	0.0	0.0	0.0	5.256414468280824E-5	0.0
8	0.0	0.0	0.0	5.256414468280824E-5	0.0
9	0.0	0.0	0.0	7.884621702421236E-5	0.0
10	0.0	0.0	0.0	2.891027957554453E-4	0.0
11	2.628207234140412E-5	0.0	0.0	4.7307730214527416E-4	0.0
12	2.628207234140412E-5	0.0	0.0	7.884621702421235E-4	0.0
13	2.628207234140412E-5	0.0	0.0	9.724366766319524E-4	0.0
14	2.628207234140412E-5	0.0	0.0	0.001103847038338973	0.0
15	2.628207234140412E-5	0.0	0.0	0.0015243601958014389	0.0
16	5.256414468280824E-5	0.0	0.0	0.0023653865107263706	0.0
17	5.256414468280824E-5	0.0	0.0	0.003653208055455173	0.0
18	5.256414468280824E-5	0.0	0.0	0.004073721212917638	0.0
19	5.256414468280824E-5	0.0	0.0	0.004704490949111338	0.0
20	5.256414468280824E-5	0.0	0.0	0.005703209698084694	0.0
21	5.256414468280824E-5	0.0	0.0	0.007937185847104044	0.0
22	5.256414468280824E-5	0.0	0.0	0.012300009855777129	0.0
23	5.256414468280824E-5	0.0	0.0	0.02060514471566083	0.0
24	5.256414468280824E-5	0.0	0.0	0.032300666907585665	0.0
25	5.256414468280824E-5	0.0	0.0	0.03866092841420546	0.0
26	5.256414468280824E-5	0.0	0.0	0.04617760110384704	0.0
27	5.256414468280824E-5	0.0	0.0	0.1026840566378659	0.0
28	5.256414468280824E-5	0.0	0.0	0.1657347481848944	0.0
29	5.256414468280824E-5	0.0	0.0	0.23632839449390586	0.0
30	5.256414468280824E-5	0.0	0.0	0.3758073524097375	0.0
31	5.256414468280824E-5	0.0	0.0	0.5970761194520188	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8560	0.0	29.284462	1
GTATTGG	1090	0.0	23.591745	1
ATTGGAC	1045	0.0	23.014355	3
TTGGACC	1835	0.0	20.264305	4
TAGTGCG	130	3.274181E-11	19.923077	7
GGACCCT	1955	0.0	18.547314	6
TGGACCC	1975	0.0	18.546837	5
TATTGGA	1495	0.0	17.200668	2
CGTCTGT	950	0.0	16.942106	34
GTATCAA	15310	0.0	16.663294	2
TACGGGT	80	3.3851352E-4	16.1875	4
GACCCTC	2105	0.0	15.995249	7
ACGTTTA	245	0.0	15.857142	26
CCGTCGA	95	7.066533E-5	15.578948	9
TAGTACG	190	2.7284841E-11	15.578948	4
CGCGAAT	85	5.368196E-4	15.235294	35
TTATGCG	165	1.6485501E-8	14.575757	4
CGAACGA	115	2.2123879E-5	14.478261	16
TCTATAC	375	0.0	14.306667	3
CGCGATA	105	1.6572184E-4	14.095238	14
>>END_MODULE