FastQCFastQC Report
Wed 25 May 2016
SRR2088216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717999
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT287001.670548120225914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275471.6034351591589984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240761.4013977889393416No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG140460.817579055633909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99150.5771248993742139No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85070.4951690891554652No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA77750.452561381001968No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA76130.4431318062466858No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT75340.43853343337219636No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA64100.3731084826009794No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59320.34528541634773946No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT58460.3402795927122193No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT56550.3291620076612385No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46660.2715950358527566No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA46600.2712457923433017No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT41450.24126905778175659No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38820.225960550617317No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.20878941140245133No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA33960.1976718263514705No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC31230.1817812466712728No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29760.17322478068962788No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27610.16071022160082746No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.15908041855670463No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT27050.15745061551258177No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG26790.15593722697161058No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25590.14895235678251267No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.13236329008340517No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA22650.13183942481922284No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC21970.12788133171206734No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC21190.12334116608915373No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC20960.12200239930290994No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG20150.11728761192526886No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC19380.11280565355393105No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19140.11140867951611147No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC18960.1103609489877468No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC18750.10913859670465466No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC18740.10908038945307884No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA18660.10861473144047232No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG18090.10529691810065081No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT17620.10256117727658748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.028.8502221
GTATTGG7100.025.5352121
ATTGGAC7250.025.0068953
TCGACGG502.7028576E-422.19999919
TTGGACC12650.021.2055324
CGAATTA1151.3460522E-1020.91304215
TATTGGA8450.020.5798842
CCCCCGT656.904672E-519.92307737
CGTTTAG656.904672E-519.92307726
CATTGCG851.2460423E-619.58823429
GGACCCT13250.019.4075476
CTGTGCG1153.0540832E-919.3043469
TGGACCC13500.018.6370375
TAGGTCG902.153478E-618.521
GACCGTC609.239349E-418.522
CCGAATT1306.9667294E-1018.514
TGAACGG500.00703626718.49999825
CTCGACG500.00703626718.49999818
TACGTCC500.00703626718.4999986
TTTTTAC14000.017.8392871
Produced by FastQC (version 0.11.5)