##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088216_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1717999
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45221271956503	31.0	31.0	33.0	27.0	34.0
2	30.835197226540878	31.0	31.0	34.0	27.0	34.0
3	31.0006612343779	31.0	31.0	34.0	27.0	34.0
4	35.04468803532482	35.0	35.0	37.0	32.0	37.0
5	34.92148307420435	35.0	35.0	37.0	32.0	37.0
6	34.97479917043025	36.0	35.0	37.0	32.0	37.0
7	34.76650743102877	35.0	35.0	37.0	32.0	37.0
8	34.8996803839816	36.0	35.0	37.0	32.0	37.0
9	36.327537443269755	38.0	35.0	39.0	32.0	39.0
10	36.21614855421918	38.0	35.0	39.0	32.0	39.0
11	36.2624396172524	38.0	35.0	39.0	32.0	39.0
12	36.08553439204563	38.0	35.0	39.0	31.0	39.0
13	36.144744554566095	38.0	35.0	39.0	31.0	39.0
14	36.98300639290244	39.0	36.0	40.0	31.0	41.0
15	37.05183763203588	39.0	36.0	40.0	31.0	41.0
16	36.99580558545145	39.0	36.0	40.0	31.0	41.0
17	37.15372476933921	39.0	36.0	40.0	31.0	41.0
18	37.18780569720937	39.0	36.0	40.0	31.0	41.0
19	37.3136206714905	39.0	36.0	40.0	31.0	41.0
20	37.276777809533066	39.0	36.0	40.0	31.0	41.0
21	37.16726843263587	39.0	36.0	40.0	31.0	41.0
22	37.11952218831326	39.0	36.0	40.0	31.0	41.0
23	36.97034689775722	39.0	35.0	40.0	31.0	41.0
24	36.91304593308843	39.0	35.0	40.0	31.0	41.0
25	36.80039743911376	39.0	35.0	40.0	30.0	41.0
26	36.502560828033076	38.0	35.0	40.0	30.0	41.0
27	36.33030461600967	38.0	35.0	40.0	30.0	41.0
28	36.149225348792406	38.0	35.0	40.0	30.0	41.0
29	36.02022236334247	38.0	35.0	40.0	30.0	41.0
30	35.826475451964754	38.0	34.0	40.0	29.0	41.0
31	35.508000877765355	38.0	34.0	40.0	28.0	41.0
32	35.18723992272405	37.0	34.0	40.0	27.0	41.0
33	35.25582610932835	38.0	34.0	40.0	27.0	41.0
34	35.18308799946915	38.0	34.0	40.0	27.0	41.0
35	35.04327767361914	38.0	33.0	40.0	26.0	41.0
36	34.91238178834796	38.0	33.0	40.0	25.0	41.0
37	34.75373501381549	38.0	33.0	40.0	25.0	41.0
38	34.57379369836653	37.0	33.0	40.0	24.0	41.0
39	34.45113763162843	38.0	33.0	40.0	23.0	41.0
40	34.225253914583185	38.0	33.0	40.0	22.0	41.0
41	34.03930328248154	38.0	33.0	40.0	21.0	41.0
42	33.771344453634725	37.0	33.0	40.0	18.0	41.0
43	33.40844144845253	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	14.0
16	24.0
17	61.0
18	164.0
19	404.0
20	807.0
21	1660.0
22	2946.0
23	4917.0
24	8093.0
25	12430.0
26	18696.0
27	26886.0
28	36997.0
29	49517.0
30	62134.0
31	73973.0
32	89678.0
33	108080.0
34	128334.0
35	152870.0
36	186518.0
37	239686.0
38	272558.0
39	240549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16358565982867	24.47725522541049	14.56246482099233	20.796694293768507
2	17.458275586889165	23.495473513081205	36.78308311006002	22.26316778996961
3	20.63918547100435	26.360550850146012	32.75525771551672	20.245005963332925
4	12.852102940688557	15.573874024373705	34.6574124897628	36.91661054517494
5	10.42387102670025	41.82854588390331	34.1153865630888	13.632196526307641
6	31.327899492374563	35.31748272263255	15.162348755732689	18.192269029260203
7	23.2345886115184	36.840882910874804	21.037788729795533	18.886739747811262
8	28.21159965750853	33.38709743137219	20.15140870279901	18.24989420832026
9	24.900945809630855	13.576201150291705	25.21014273000159	36.31271031007585
10	16.75414246457652	28.086512273872106	33.071672335082845	22.087672926468525
11	30.108632193615946	21.6364503122528	26.025800946333494	22.22911654779776
12	20.037497111465143	27.982437708054547	32.221031560553875	19.759033619926438
13	31.571264011213046	20.929523241864516	30.17731675047541	17.32189599644703
14	20.506531144663064	23.020851583732004	29.680576065527397	26.79204120607754
15	22.655542872842187	28.873066864416103	28.64623320502515	19.825157057716563
16	20.91083871410868	28.66555801254832	28.348561320466427	22.075041952876575
17	18.0868556966564	27.091808551692985	28.865732750717548	25.955603000933063
18	19.409906524974694	26.398327356418715	34.61922853272906	19.572537585877523
19	18.420383248185825	24.368349457712142	36.41404913506934	20.79721815903269
20	24.220910489470597	22.990059947648398	34.799787427117245	17.989242135763757
21	23.348907653613303	24.164216626435756	33.03453610857748	19.452339611373464
22	20.95455236004212	23.78796495224968	34.17696983525602	21.080512852452184
23	19.660896193769613	26.56980591956107	35.61742469000273	18.15187319666659
24	18.053095490742425	25.297453607365313	33.62539791932358	23.024052982568673
25	19.487613205828406	26.646697698892723	35.05071888866059	18.81497020661828
26	21.562410688248363	24.988314894246155	33.211311531613234	20.237962885892248
27	19.725389828515617	27.036977320708566	32.734826970213604	20.50280588056221
28	19.332141636869405	26.37452059052421	32.93692254768484	21.35641522492155
29	18.100185157267262	26.180224784764132	34.256422733657004	21.4631673243116
30	17.380394284280722	26.274636946820106	36.90520192386607	19.439766845033088
31	19.151175291720193	25.705893891672808	35.187680551618485	19.955250264988514
32	20.74430776735027	26.138490185384278	32.50351135245131	20.613690694814142
33	21.970559936297985	26.07550993917924	31.735699496914727	20.21823062760805
34	19.44779944575055	29.734767016744478	31.84827232146235	18.969161216042618
35	17.81659942758989	30.6298199242258	32.018470325070034	19.535110323114274
36	19.00391094523338	25.944252586875777	34.97504946161203	20.076787006278813
37	19.19366658537054	26.443845427151004	33.64466451959518	20.717823467883274
38	19.638020743900316	25.84099292258028	34.38197577530604	20.139010558213364
39	20.970442939722318	25.140002991852732	34.117598438648685	19.77195562977627
40	18.720034179298125	26.237442513063165	32.84914601230851	22.193377295330208
41	19.95350404744124	25.81596380440268	32.549087630435174	21.68144451772091
42	16.851057538450256	27.858339847694907	33.12941392864606	22.16118868520878
43	17.801581956683325	27.736221033888842	32.49908760133155	21.96310940809628
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	214.0
2	249.0
3	815.5
4	1382.0
5	1382.0
6	2515.0
7	3648.0
8	4150.5
9	4653.0
10	7374.0
11	10095.0
12	10095.0
13	18342.5
14	26590.0
15	43529.5
16	60469.0
17	58567.0
18	56665.0
19	56665.0
20	59052.5
21	61440.0
22	48177.0
23	34914.0
24	36967.0
25	39020.0
26	39020.0
27	41076.5
28	43133.0
29	43696.5
30	44260.0
31	46652.0
32	49044.0
33	49044.0
34	52584.0
35	56124.0
36	60628.5
37	65133.0
38	68199.0
39	71265.0
40	71265.0
41	75574.0
42	79883.0
43	95716.5
44	111550.0
45	150409.5
46	189269.0
47	189269.0
48	191164.0
49	193059.0
50	172071.5
51	151084.0
52	138249.5
53	125415.0
54	125415.0
55	105621.0
56	85827.0
57	67309.5
58	48792.0
59	41272.5
60	33753.0
61	33753.0
62	28602.0
63	23451.0
64	20114.0
65	16777.0
66	14084.5
67	11392.0
68	11392.0
69	9411.0
70	7430.0
71	6175.5
72	4921.0
73	4044.5
74	3168.0
75	3168.0
76	2515.0
77	1862.0
78	1444.5
79	1027.0
80	786.5
81	546.0
82	546.0
83	417.5
84	289.0
85	208.0
86	127.0
87	95.0
88	63.0
89	63.0
90	50.5
91	38.0
92	23.5
93	9.0
94	6.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1717999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.63804966549199
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28321388323236	31.000010344301447
2	12.739490269043177	10.354160765328572
3	4.505304298122672	5.492603394757911
4	1.9902838458969032	3.2352501511193905
5	1.0764367295956785	2.187214463953448
6	0.6695410643082534	1.6325305814667104
7	0.4434442649208276	1.2614497043211126
8	0.3198012983058554	1.0396880834913738
9	0.2276513345635426	0.8326175616367901
>10	1.4713556397055507	11.573002325110163
>50	0.1593906840954618	4.476068961057118
>100	0.0938854129402337	7.560227038460154
>500	0.008574642020859304	2.421715201274666
>1k	0.009737305345721585	7.510010281038045
>5k	0.0013079962404700636	3.8499743640984208
>10k+	5.813316624311393E-4	5.573476778584747
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28700	1.670548120225914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27547	1.6034351591589984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24076	1.4013977889393416	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14046	0.817579055633909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9915	0.5771248993742139	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8507	0.4951690891554652	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7775	0.452561381001968	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7613	0.4431318062466858	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7534	0.43853343337219636	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6410	0.3731084826009794	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5932	0.34528541634773946	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5846	0.3402795927122193	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5655	0.3291620076612385	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4666	0.2715950358527566	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4660	0.2712457923433017	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4145	0.24126905778175659	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3882	0.225960550617317	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3587	0.20878941140245133	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3396	0.1976718263514705	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3123	0.1817812466712728	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2976	0.17322478068962788	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2761	0.16071022160082746	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2733	0.15908041855670463	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2705	0.15745061551258177	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2679	0.15593722697161058	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2559	0.14895235678251267	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2274	0.13236329008340517	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2265	0.13183942481922284	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2197	0.12788133171206734	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2119	0.12334116608915373	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2096	0.12200239930290994	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2015	0.11728761192526886	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1938	0.11280565355393105	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1914	0.11140867951611147	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1896	0.1103609489877468	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	1875	0.10913859670465466	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1874	0.10908038945307884	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1866	0.10861473144047232	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1809	0.10529691810065081	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	1762	0.10256117727658748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.820725157581582E-5	0.0	0.0	0.0
3	0.0	5.820725157581582E-5	0.0	0.0	0.0
4	0.0	5.820725157581582E-5	0.0	0.0	0.0
5	0.0	5.820725157581582E-5	0.0	0.0	0.0
6	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.0	0.0
7	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.0	0.0
8	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.0	0.0
9	1.1641450315163163E-4	5.820725157581582E-5	0.0	1.7462175472744744E-4	0.0
10	1.1641450315163163E-4	5.820725157581582E-5	0.0	5.820725157581582E-4	0.0
11	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.0011641450315163164	0.0
12	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.001629803044122843	0.0
13	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.001920839302001922	0.0
14	1.1641450315163163E-4	5.820725157581582E-5	0.0	0.002561119069335896	0.0
15	1.7462175472744744E-4	5.820725157581582E-5	0.0	0.0044237511197620025	0.0
16	1.7462175472744744E-4	5.820725157581582E-5	0.0	0.006810248434370451	0.0
17	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.01100117054782919	0.0
18	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.012572766340376216	0.0
19	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.014318983887650691	0.0
20	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.018160662491654534	0.0
21	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.024563460164994274	0.0
22	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.04004658908416128	0.0
23	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.0618161011735164	0.0
24	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.09144359222560665	0.0
25	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.10378352955967961	0.0
26	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.11752044093157214	0.0
27	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.20721781560990432	0.0
28	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.30232846468478736	0.0
29	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.4089641495716819	0.0
30	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.6447617257053119	0.0
31	1.7462175472744744E-4	1.1641450315163163E-4	0.0	0.9573928739190186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6810	0.0	28.850222	1
GTATTGG	710	0.0	25.535212	1
ATTGGAC	725	0.0	25.006895	3
TCGACGG	50	2.7028576E-4	22.199999	19
TTGGACC	1265	0.0	21.205532	4
CGAATTA	115	1.3460522E-10	20.913042	15
TATTGGA	845	0.0	20.579884	2
CCCCCGT	65	6.904672E-5	19.923077	37
CGTTTAG	65	6.904672E-5	19.923077	26
CATTGCG	85	1.2460423E-6	19.588234	29
GGACCCT	1325	0.0	19.407547	6
CTGTGCG	115	3.0540832E-9	19.304346	9
TGGACCC	1350	0.0	18.637037	5
TAGGTCG	90	2.153478E-6	18.5	21
GACCGTC	60	9.239349E-4	18.5	22
CCGAATT	130	6.9667294E-10	18.5	14
TGAACGG	50	0.007036267	18.499998	25
CTCGACG	50	0.007036267	18.499998	18
TACGTCC	50	0.007036267	18.499998	6
TTTTTAC	1400	0.0	17.839287	1
>>END_MODULE