FastQCFastQC Report
Wed 25 May 2016
SRR2088215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394577
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT341401.4257215366221256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328801.3731026398399384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT288791.2060167620418971No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG181470.7578373967510755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118950.4967474422413645No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT100790.42090941322830716No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA99460.4153551963457429No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT91700.3829486376925862No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA91540.3822804612255108No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA76810.3207664652253822No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG72410.3023916123808088No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT72260.30176519694292564No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT63080.2634285721444748No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA55060.2299362267323206No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53830.22479962014167848No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48340.20187281511515395No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43480.1815769549277388No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43040.17973946964328147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41830.17468638511102377No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35940.15008913891681078No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34860.14557894776405186No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG32790.13693441472126391No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT32750.1367673706044951No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31530.1316725250430452No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31160.1301273669629333No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29740.12419730081763918No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.11972887069407248No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26150.10920509133763501No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA25870.10803578252025306No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA25570.10678295164448669No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA23950.10001766491534832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75100.030.1271651
ATTGGAC9600.021.9687483
GTATTGG9950.020.6381911
TATACCG902.1541982E-618.55
TTGGACC16600.018.1656634
TGGACCC16350.018.1039755
GGACCCT16250.017.9876926
TATTGGA11650.017.7854082
CGTCTGT7050.017.31914934
GTATCAA136500.016.9956052
CGTGACT1005.883143E-616.65000212
ACCGACC1005.883143E-616.6500028
AGCCGTC1251.6597915E-716.27999912
GACCCTC18000.015.9305557
TGCGCGC750.004106133714.79999919
TCCCGTA750.004106133714.7999992
CTAGCGG750.004106133714.79999929
CGGACCA1252.9618022E-614.7999999
TATACTG2900.014.6724145
ACAGCGT908.2786306E-414.3888888
Produced by FastQC (version 0.11.5)