##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088215_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2394577
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.425881898974225	31.0	30.0	33.0	27.0	34.0
2	30.787177443030647	31.0	31.0	34.0	27.0	34.0
3	30.970997800446593	31.0	31.0	34.0	27.0	34.0
4	35.02070135978087	35.0	35.0	37.0	32.0	37.0
5	34.86136257050828	35.0	35.0	37.0	32.0	37.0
6	34.910974673188626	35.0	35.0	37.0	32.0	37.0
7	34.716952096341025	35.0	35.0	37.0	31.0	37.0
8	34.84319944608171	35.0	35.0	37.0	32.0	37.0
9	36.29296656570242	38.0	35.0	39.0	32.0	39.0
10	36.166041434457945	38.0	35.0	39.0	32.0	39.0
11	36.201820613828666	38.0	35.0	39.0	32.0	39.0
12	36.03244664924118	38.0	35.0	39.0	30.0	39.0
13	36.11079034000577	38.0	35.0	39.0	31.0	39.0
14	36.9678385785882	39.0	36.0	40.0	31.0	41.0
15	37.03243119766038	39.0	36.0	40.0	31.0	41.0
16	36.9727396529742	38.0	36.0	40.0	31.0	41.0
17	37.102408483836605	39.0	36.0	40.0	31.0	41.0
18	37.1077956566024	39.0	36.0	40.0	31.0	41.0
19	37.22552292116729	39.0	36.0	40.0	31.0	41.0
20	37.18497087377019	39.0	36.0	40.0	31.0	41.0
21	37.07276608770568	39.0	36.0	40.0	31.0	41.0
22	37.030637143846285	39.0	36.0	40.0	31.0	41.0
23	36.9148016539038	39.0	35.0	40.0	31.0	41.0
24	36.84482729099962	39.0	35.0	40.0	31.0	41.0
25	36.73621562388681	38.0	35.0	40.0	30.0	41.0
26	36.45365590665909	38.0	35.0	40.0	30.0	41.0
27	36.29032225733397	38.0	35.0	40.0	30.0	41.0
28	36.120326053411524	38.0	35.0	40.0	30.0	41.0
29	35.99496654315146	38.0	34.0	40.0	29.0	41.0
30	35.82199110740645	38.0	34.0	40.0	29.0	41.0
31	35.541301449065955	38.0	34.0	40.0	27.0	41.0
32	35.24364595500583	38.0	34.0	40.0	27.0	41.0
33	35.35147209715954	38.0	34.0	40.0	27.0	41.0
34	35.315311221982	38.0	34.0	40.0	27.0	41.0
35	35.192556347112664	38.0	34.0	40.0	26.0	41.0
36	35.08817883074965	38.0	34.0	40.0	25.0	41.0
37	34.93707907492639	38.0	33.0	40.0	25.0	41.0
38	34.790494103969095	38.0	33.0	40.0	25.0	41.0
39	34.69888042856839	38.0	33.0	40.0	24.0	41.0
40	34.510602916506755	38.0	33.0	40.0	23.0	41.0
41	34.366618822447556	38.0	33.0	40.0	22.0	41.0
42	34.152077381516655	38.0	33.0	40.0	21.0	41.0
43	33.7947123855278	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	11.0
16	45.0
17	96.0
18	234.0
19	540.0
20	1173.0
21	2224.0
22	4109.0
23	6756.0
24	11183.0
25	17285.0
26	25677.0
27	37540.0
28	51121.0
29	67428.0
30	85781.0
31	103531.0
32	125195.0
33	149270.0
34	178539.0
35	210452.0
36	259005.0
37	325649.0
38	385063.0
39	346668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.26356220743789	24.163766711197844	14.12094912796707	21.451721953397197
2	17.57688309876859	23.43992279220923	36.457670812005624	22.52552329701655
3	20.46808267180383	26.50656044888095	32.147264422902246	20.87809245641297
4	13.107158383296923	15.869733986420147	35.17147287391468	35.85163475636824
5	10.888770751577418	41.355404315668274	34.41739396979091	13.338430962963397
6	31.455158886099717	35.851384190193095	15.463566216496694	17.2298907072105
7	24.034641608935527	35.76690162813724	21.535661622073544	18.66279514085369
8	27.882628121793534	33.679476583964515	20.250883558975133	18.187011735266815
9	25.171376823547543	13.545064535406462	24.27773255986339	37.005826081182605
10	16.39099515279734	27.84228696759386	33.598460187331625	22.168257692277173
11	30.949224017436066	21.5932918423588	25.669043008431135	21.788441131774007
12	19.881423733711635	27.52987270820692	32.25935102525415	20.3293525328273
13	31.20346516315825	20.761495662908313	29.644484182383778	18.390554991549656
14	20.706955758783284	22.511658635324736	29.136962394610823	27.644423211281154
15	22.808412508764597	28.749503565765476	27.95750564713517	20.48457827833475
16	21.189629734186873	28.488246567138997	27.92121531276714	22.40090838590699
17	18.76181054106842	26.893893994638717	28.708786562303068	25.63550890198979
18	19.939930935609922	26.295792534547857	33.52124404435523	20.243032485486996
19	19.222601737175292	24.42272685321875	34.929091860483084	21.425579549122872
20	24.06095105732662	23.384171818237625	33.684654951584356	18.870222172851406
21	23.402838998286548	24.385016643858183	32.13373384944397	20.0784105084113
22	21.41367765580309	23.789086757285315	33.40890687582817	21.388328711083417
23	20.302416668998326	26.15313685882726	34.57779808291819	18.966648389256225
24	19.01317017577635	25.064050978523554	32.847304555251306	23.075474290448796
25	20.185151699026594	26.26413767442016	33.98587725514778	19.56483337140547
26	21.98785004616682	24.954595321010768	32.38033272682399	20.67722190599843
27	20.330354797527914	26.69594671626763	31.93866808208715	21.035030404117304
28	20.06354358201887	25.968511348768487	32.23471201803074	21.733233051181898
29	19.074851215893247	25.88845545580702	33.29377171834524	21.742921609954493
30	18.28702940018216	26.066315679136647	35.44705390555409	20.199601015127097
31	19.948658990711092	25.40294173041836	34.172047923286655	20.476351355583887
32	21.18202922687389	25.68136251204284	31.831008148829625	21.305600112253646
33	22.285439140190523	25.59312145735969	31.295214144293542	20.826225258156242
34	20.276357786782384	28.740190856255616	31.282811118623457	19.700640238338547
35	18.854937636167055	29.61733951340884	31.4209148421621	20.106808008262004
36	19.809135392179915	25.560255527385422	33.90648953865338	20.724119541781285
37	20.02541576236638	25.939529194509092	32.871734757328746	21.163320285795777
38	20.41579786325518	25.335664712389704	33.479942386484126	20.76859503787099
39	21.33583509738881	24.90289516687081	33.39395642737736	20.367313308363023
40	19.470912816752186	25.814538434136804	32.24703152164244	22.467517227468566
41	20.5041642010259	25.386571406975012	32.003773526597804	22.10549086540128
42	17.91924001608635	27.350843176059907	32.386972730465544	22.3429440773882
43	18.756214563156668	27.108503923657494	31.878198111816825	22.25708340136901
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	274.0
2	372.0
3	1091.0
4	1810.0
5	1810.0
6	3178.5
7	4547.0
8	5146.5
9	5746.0
10	8816.0
11	11886.0
12	11886.0
13	21733.5
14	31581.0
15	51381.0
16	71181.0
17	69366.0
18	67551.0
19	67551.0
20	71011.5
21	74472.0
22	59495.0
23	44518.0
24	47508.5
25	50499.0
26	50499.0
27	54409.5
28	58320.0
29	60780.0
30	63240.0
31	67375.5
32	71511.0
33	71511.0
34	77644.0
35	83777.0
36	91944.5
37	100112.0
38	104172.5
39	108233.0
40	108233.0
41	114802.5
42	121372.0
43	140503.5
44	159635.0
45	206016.0
46	252397.0
47	252397.0
48	252750.0
49	253103.0
50	227086.5
51	201070.0
52	186188.5
53	171307.0
54	171307.0
55	146191.0
56	121075.0
57	98876.0
58	76677.0
59	66111.0
60	55545.0
61	55545.0
62	48835.5
63	42126.0
64	36541.5
65	30957.0
66	26251.5
67	21546.0
68	21546.0
69	18256.5
70	14967.0
71	12525.5
72	10084.0
73	7966.0
74	5848.0
75	5848.0
76	4497.5
77	3147.0
78	2472.5
79	1798.0
80	1413.0
81	1028.0
82	1028.0
83	809.5
84	591.0
85	480.0
86	369.0
87	295.5
88	222.0
89	222.0
90	187.5
91	153.0
92	85.0
93	17.0
94	11.5
95	6.0
96	6.0
97	4.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2394577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.57953788647824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92787377072833	31.15466366203258
2	13.251871496736168	11.020133859305652
3	4.845139809845233	6.043760228662314
4	2.1936224612674375	3.648392329475963
5	1.2319366903624356	2.561167914533375
6	0.734426252529346	1.8322262515120906
7	0.45647830168503944	1.3286109787487839
8	0.3330851481590469	1.1079621229841847
9	0.24790332136867677	0.9276934988729416
>10	1.5165818101158473	12.088519555942275
>50	0.15578904496961654	4.496710785221585
>100	0.08944009164926678	7.093452237346091
>500	0.006909759228685867	1.9690082456478302
>1k	0.007417829760206885	6.123981957324186
>5k	9.14526956737835E-4	2.8567794461617924
>10k+	6.096846378252235E-4	5.746936926228308
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34140	1.4257215366221256	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32880	1.3731026398399384	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28879	1.2060167620418971	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18147	0.7578373967510755	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11895	0.4967474422413645	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10079	0.42090941322830716	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9946	0.4153551963457429	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9170	0.3829486376925862	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9154	0.3822804612255108	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7681	0.3207664652253822	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7241	0.3023916123808088	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7226	0.30176519694292564	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6308	0.2634285721444748	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5506	0.2299362267323206	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5383	0.22479962014167848	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4834	0.20187281511515395	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4348	0.1815769549277388	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4304	0.17973946964328147	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4183	0.17468638511102377	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3594	0.15008913891681078	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3486	0.14557894776405186	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3279	0.13693441472126391	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3275	0.1367673706044951	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3153	0.1316725250430452	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3116	0.1301273669629333	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2974	0.12419730081763918	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2867	0.11972887069407248	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2615	0.10920509133763501	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2587	0.10803578252025306	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2557	0.10678295164448669	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2395	0.10001766491534832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.1761029192212235E-5	0.0
2	0.0	0.0	0.0	4.1761029192212235E-5	0.0
3	0.0	0.0	0.0	4.1761029192212235E-5	0.0
4	0.0	0.0	0.0	4.1761029192212235E-5	0.0
5	0.0	0.0	0.0	8.352205838442447E-5	0.0
6	4.1761029192212235E-5	0.0	0.0	8.352205838442447E-5	0.0
7	8.352205838442447E-5	0.0	0.0	8.352205838442447E-5	0.0
8	8.352205838442447E-5	0.0	0.0	1.252830875766367E-4	0.0
9	8.352205838442447E-5	0.0	0.0	3.340882335376979E-4	0.0
10	8.352205838442447E-5	0.0	0.0	7.516985254598203E-4	0.0
11	8.352205838442447E-5	0.0	0.0	0.001211069846574155	0.0
12	8.352205838442447E-5	0.0	0.0	0.0015033970509196405	0.0
13	8.352205838442447E-5	0.0	0.0	0.0016286801384962771	0.0
14	8.352205838442447E-5	0.0	0.0	0.0022550955763794606	0.0
15	8.352205838442447E-5	0.0	0.0	0.0032573602769925543	0.0
16	2.088051459610612E-4	0.0	0.0	0.005345411736603166	0.0
17	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.007600507312982627	0.0
18	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.008352205838442447	0.0
19	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.009229187451478904	0.0
20	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.01231950361170261	0.0
21	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.018583657990534446	0.0
22	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.03407699982084519	0.0
23	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.05520808059210457	0.0
24	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.08277035985896465	0.0
25	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.09220835245640462	0.0
26	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.1041102457761851	0.0
27	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.18729821592707188	0.0
28	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.27320065297545243	0.0
29	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.36945982526350163	0.0
30	2.088051459610612E-4	4.1761029192212235E-5	0.0	0.571207357291079	0.0
31	4.176102919221224E-4	4.1761029192212235E-5	0.0	0.8497951830323268	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7510	0.0	30.127165	1
ATTGGAC	960	0.0	21.968748	3
GTATTGG	995	0.0	20.638191	1
TATACCG	90	2.1541982E-6	18.5	5
TTGGACC	1660	0.0	18.165663	4
TGGACCC	1635	0.0	18.103975	5
GGACCCT	1625	0.0	17.987692	6
TATTGGA	1165	0.0	17.785408	2
CGTCTGT	705	0.0	17.319149	34
GTATCAA	13650	0.0	16.995605	2
CGTGACT	100	5.883143E-6	16.650002	12
ACCGACC	100	5.883143E-6	16.650002	8
AGCCGTC	125	1.6597915E-7	16.279999	12
GACCCTC	1800	0.0	15.930555	7
TGCGCGC	75	0.0041061337	14.799999	19
TCCCGTA	75	0.0041061337	14.799999	2
CTAGCGG	75	0.0041061337	14.799999	29
CGGACCA	125	2.9618022E-6	14.799999	9
TATACTG	290	0.0	14.672414	5
ACAGCGT	90	8.2786306E-4	14.388888	8
>>END_MODULE