FastQCFastQC Report
Wed 25 May 2016
SRR2088214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718389
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114901.5994120177229885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98211.367086634121625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65860.9167735029350393No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61530.8564997515273759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.5161548965811003No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT34000.47328118888234644No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT27210.3787641514555485No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27130.3776505486581782No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA21280.29621834410048037No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19550.2721366836073492No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17440.2427654098267095No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT15010.20893972485658885No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14610.2033717108697377No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT14570.20281490947105257No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.1745572384877831No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10140.1411491545666763No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9450.13154433043935806No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA9180.12778592099823355No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC8660.12054750281532706No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8510.11845949757025791No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.11539708987748977No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT8190.114005086380777No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG8070.11233468218472165No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7810.1087154730932684No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7740.10774107064556947No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7720.10746266994622691No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG7700.10718426924688434No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7380.10272985805740345No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC7350.10231225700838961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.029.6984481
CGACGAG250.005494988529.624
ACGTTTA405.937076E-527.7526
TATAGAC358.866144E-426.428573
ACTCGGG509.084892E-625.90000216
GCTATGC400.00193048623.1253
AACACCG400.00193048623.1255
TACTCGG603.7237827E-521.58333415
TACCAAC450.003824432120.5555557
CCGTATA450.003824432120.5555552
TCTAATG450.003824432120.5555553
GAGTATA555.1405135E-420.1818181
GCGCTAC555.1405135E-420.1818181
TCACGTT555.1405135E-420.18181824
CGGACCA555.1405135E-420.1818189
GTATCAA34950.019.3733882
GTATTGG2450.018.877551
TAGACGT500.007032376718.516
TACGGAC500.007032376718.530
ATCACGT609.2322193E-418.523
Produced by FastQC (version 0.11.5)