##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088214_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718389
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.956987091951575	31.0	30.0	31.0	27.0	34.0
2	30.23985194650809	31.0	30.0	31.0	27.0	34.0
3	30.32989230068946	31.0	30.0	33.0	27.0	34.0
4	34.327133349758974	35.0	35.0	37.0	30.0	37.0
5	34.07963512804344	35.0	33.0	37.0	30.0	37.0
6	34.30269672837418	35.0	35.0	37.0	30.0	37.0
7	34.12221929901488	35.0	35.0	37.0	30.0	37.0
8	34.29726095471952	35.0	35.0	37.0	30.0	37.0
9	35.68013012448687	37.0	35.0	39.0	30.0	39.0
10	35.4113050171982	37.0	34.0	39.0	30.0	39.0
11	35.56993077566611	37.0	35.0	39.0	30.0	39.0
12	35.31720418881692	37.0	34.0	39.0	30.0	39.0
13	35.444273228014346	37.0	34.0	39.0	30.0	39.0
14	36.16589897673823	38.0	34.0	40.0	29.0	41.0
15	36.28381420094127	38.0	34.0	40.0	30.0	41.0
16	36.150060760952634	38.0	34.0	40.0	29.0	41.0
17	36.26705447884085	38.0	34.0	40.0	30.0	41.0
18	36.268131889547305	38.0	34.0	40.0	30.0	41.0
19	36.37902584811293	38.0	35.0	40.0	30.0	41.0
20	36.355187788231724	38.0	35.0	40.0	30.0	41.0
21	36.25602841914339	38.0	34.0	40.0	29.0	41.0
22	36.19196285021068	38.0	34.0	40.0	29.0	41.0
23	36.009259607260134	38.0	34.0	40.0	29.0	41.0
24	35.94573970369814	38.0	34.0	40.0	29.0	41.0
25	35.81726056495854	38.0	34.0	40.0	28.0	41.0
26	35.52037266717614	38.0	34.0	40.0	27.0	41.0
27	35.30697992313357	38.0	34.0	40.0	27.0	41.0
28	35.116055507531435	37.0	33.0	40.0	27.0	41.0
29	34.952773497367026	37.0	33.0	40.0	26.0	41.0
30	34.750146508368026	37.0	33.0	40.0	26.0	41.0
31	34.46406612573411	37.0	33.0	39.0	25.0	40.0
32	34.18501675276208	36.0	33.0	39.0	24.0	40.0
33	34.365794854876675	37.0	33.0	39.0	25.0	40.0
34	34.30019669009408	37.0	33.0	39.0	25.0	41.0
35	34.185698834475474	37.0	33.0	39.0	24.0	41.0
36	34.05382738321439	37.0	33.0	39.0	24.0	40.0
37	33.91446556113749	37.0	33.0	40.0	23.0	41.0
38	33.76262999572655	37.0	32.0	39.0	23.0	41.0
39	33.68089433440657	37.0	32.0	40.0	22.0	41.0
40	33.501687804239765	37.0	32.0	40.0	21.0	41.0
41	33.35900048580922	37.0	31.0	40.0	19.0	41.0
42	33.1151409612341	37.0	31.0	40.0	18.0	41.0
43	32.728141717091994	36.0	31.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	7.0
15	15.0
16	33.0
17	86.0
18	180.0
19	341.0
20	671.0
21	1302.0
22	2209.0
23	3508.0
24	5282.0
25	7884.0
26	11017.0
27	15279.0
28	20264.0
29	25501.0
30	31841.0
31	37722.0
32	44204.0
33	52184.0
34	61367.0
35	71876.0
36	86664.0
37	100330.0
38	95986.0
39	42632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.882570585017305	25.861476164028126	14.604900687510527	19.65105256344404
2	21.76856828264353	25.49162083495154	31.82955195583451	20.910258926570425
3	24.659202743917294	26.174259349739486	30.570902394106813	18.59563551223641
4	17.593392994603203	18.381266973742637	32.329559611853746	31.695780419800414
5	11.38213419192109	42.80034911447698	33.30911247249053	12.508404221111402
6	32.07899898244545	36.49415567331905	14.827203645935558	16.599641698299948
7	23.941764141711523	36.18624449984618	21.621015911991968	18.250975446450322
8	27.96896945805128	35.114123406678	20.00712705790317	16.909780077367554
9	25.485496019566	13.536955604832478	23.72809160496611	37.24945677063541
10	16.859389550786553	28.957431141067026	33.17868174484854	21.004497563297882
11	32.207898506241044	21.511047635751662	24.51721838725259	21.763835470754703
12	18.469241594734886	28.485263554982048	32.690923719600384	20.35457113068268
13	31.290289801207983	20.654965485273298	29.655381694318816	18.399363019199903
14	20.455352183844685	22.732252303417784	29.50393171387646	27.30846379886106
15	23.664616245516008	29.164561261377887	27.28925415060643	19.881568342499676
16	21.070617729391735	28.645065556404674	28.393669724898352	21.89064698930524
17	18.71604381470206	27.608162151703326	29.605687169486174	24.070106864108443
18	19.52897385678233	26.43052719348431	33.1953857868091	20.845113162924264
19	19.28815725185102	25.008178020543188	34.8674603870605	20.836204340545304
20	23.445654095483086	24.232692872524495	33.90962278097243	18.41203025101999
21	22.544888632760244	24.798681494287912	32.552976173076146	20.103453699875693
22	21.226104519974555	24.08263489557886	34.025576672248604	20.665683912197988
23	20.162474648136318	26.542165873920677	34.68427272689309	18.611086751049918
24	19.37780227703932	25.334463640172665	33.048390217556225	22.23934386523179
25	20.099138489035884	26.86497148480837	33.72128470786719	19.31460531828856
26	21.611411087864653	25.166727218818775	32.60197469616043	20.619886997156136
27	20.99252633322615	26.890584349147883	32.16224079154887	19.954648526077097
28	20.396192035234392	26.354802203263134	32.75077986995903	20.498225891543438
29	19.350936609552765	25.6846917199456	34.23047958696473	20.733892083536915
30	19.012262158802542	25.957246004601963	35.31443270985496	19.716059126740525
31	19.993485423635384	25.835167297940252	33.80229931137587	20.36904796704849
32	21.20174445878208	26.41966956620995	32.066053349926015	20.312532625081957
33	22.247835086561736	26.29675565745021	31.327177893870868	20.128231362117184
34	20.92863337272703	28.42874821301551	31.512871160332356	19.129747253925103
35	19.512409015171446	28.811131573562516	31.819668731007848	19.85679068025819
36	20.17834348799884	25.600336308044806	34.21725555374595	20.004064650210402
37	20.439065742933145	25.904628272426223	33.21849304485453	20.437812939786106
38	20.683779957655254	25.66186286259951	33.381357453969926	20.27299972577531
39	21.539305306734928	25.40086220696586	33.169216121070896	19.890616365228308
40	20.324782255853027	25.847277728361657	31.940216233823183	21.88772378196214
41	20.992247932526805	25.89126503885778	31.710118055816555	21.40636897279886
42	18.74973029932251	27.769356156622667	31.31673786764552	22.164175676409297
43	20.106794508267807	26.676633411703133	31.37381001101075	21.842762069018317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	86.0
2	93.0
3	208.5
4	324.0
5	324.0
6	722.5
7	1121.0
8	1262.0
9	1403.0
10	2295.0
11	3187.0
12	3187.0
13	6283.0
14	9379.0
15	15332.5
16	21286.0
17	19920.5
18	18555.0
19	18555.0
20	21571.5
21	24588.0
22	19647.5
23	14707.0
24	15575.0
25	16443.0
26	16443.0
27	17496.5
28	18550.0
29	19776.5
30	21003.0
31	22503.5
32	24004.0
33	24004.0
34	25927.5
35	27851.0
36	30000.5
37	32150.0
38	33265.0
39	34380.0
40	34380.0
41	36414.0
42	38448.0
43	43315.0
44	48182.0
45	60005.0
46	71828.0
47	71828.0
48	70441.0
49	69054.0
50	62036.5
51	55019.0
52	50875.5
53	46732.0
54	46732.0
55	40318.5
56	33905.0
57	29014.5
58	24124.0
59	21264.0
60	18404.0
61	18404.0
62	16054.5
63	13705.0
64	11899.0
65	10093.0
66	8546.0
67	6999.0
68	6999.0
69	5949.0
70	4899.0
71	4141.5
72	3384.0
73	2676.5
74	1969.0
75	1969.0
76	1530.5
77	1092.0
78	864.5
79	637.0
80	512.0
81	387.0
82	387.0
83	282.0
84	177.0
85	146.5
86	116.0
87	92.0
88	68.0
89	68.0
90	60.0
91	52.0
92	29.5
93	7.0
94	6.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	718389.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.87945088929769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.22462919761095	51.63083449486249
2	8.305555208410711	9.946641704207595
3	2.1958916272579754	3.9446635455784174
4	0.91956396472163	2.202519410604669
5	0.5242394128925194	1.5695584089265937
6	0.3443907163300285	1.2373156191128396
7	0.22946483958194408	0.9618160014797315
8	0.18450517952050707	0.8838455068735407
9	0.13666423871559935	0.7365041613444591
>10	0.8288768095575858	9.08859851722849
>50	0.057194812799875795	2.3394825020840173
>100	0.03968608924831836	4.801967534449085
>500	0.005602742011442464	2.3939944106407602
>1k	0.002801371005721232	3.5025063618483525
>5k	7.00342751430308E-4	3.153597682123996
>10k+	2.334475838101027E-4	1.6061541386349607
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11490	1.5994120177229885	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9821	1.367086634121625	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6586	0.9167735029350393	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6153	0.8564997515273759	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3708	0.5161548965811003	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3400	0.47328118888234644	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2721	0.3787641514555485	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2713	0.3776505486581782	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2128	0.29621834410048037	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1955	0.2721366836073492	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1744	0.2427654098267095	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1501	0.20893972485658885	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1461	0.2033717108697377	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1457	0.20281490947105257	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.1745572384877831	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1014	0.1411491545666763	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	945	0.13154433043935806	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	918	0.12778592099823355	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	866	0.12054750281532706	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	851	0.11845949757025791	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.11539708987748977	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	819	0.114005086380777	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	807	0.11233468218472165	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	781	0.1087154730932684	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	774	0.10774107064556947	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	772	0.10746266994622691	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	770	0.10718426924688434	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	738	0.10272985805740345	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	735	0.10231225700838961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3920034967127836E-4	0.0
5	0.0	0.0	0.0	1.3920034967127836E-4	0.0
6	0.0	0.0	0.0	1.3920034967127836E-4	0.0
7	0.0	0.0	0.0	1.3920034967127836E-4	0.0
8	0.0	0.0	0.0	1.3920034967127836E-4	0.0
9	0.0	0.0	0.0	1.3920034967127836E-4	0.0
10	0.0	0.0	0.0	1.3920034967127836E-4	0.0
11	0.0	0.0	0.0	1.3920034967127836E-4	0.0
12	0.0	0.0	0.0	2.7840069934255673E-4	0.0
13	0.0	0.0	0.0	2.7840069934255673E-4	0.0
14	0.0	0.0	0.0	6.960017483563918E-4	0.0
15	0.0	0.0	0.0	8.352020980276702E-4	0.0
16	0.0	0.0	0.0	0.001531203846384062	0.0
17	0.0	0.0	0.0	0.002644806643754289	0.0
18	0.0	0.0	0.0	0.003340808392110681	0.0
19	0.0	0.0	0.0	0.0038976097907957945	0.0
20	0.0	0.0	0.0	0.005568013986851135	0.0
21	0.0	0.0	0.0	0.0072384181829064755	0.0
22	0.0	0.0	0.0	0.014476836365812951	0.0
23	0.0	0.0	0.0	0.022132855597733262	0.0
24	0.0	0.0	0.0	0.037444894061573884	0.0
25	0.0	0.0	0.0	0.043012908048425016	0.0
26	0.0	0.0	0.0	0.051504129378372995	0.0
27	0.0	0.0	0.0	0.09966745036463531	0.0
28	0.0	0.0	0.0	0.15075397869399448	0.0
29	0.0	0.0	0.0	0.21534294094146764	0.0
30	0.0	0.0	0.0	0.32976562837125845	0.0
31	0.0	0.0	0.0	0.4945788423820521	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2255	0.0	29.698448	1
CGACGAG	25	0.0054949885	29.6	24
ACGTTTA	40	5.937076E-5	27.75	26
TATAGAC	35	8.866144E-4	26.42857	3
ACTCGGG	50	9.084892E-6	25.900002	16
GCTATGC	40	0.001930486	23.125	3
AACACCG	40	0.001930486	23.125	5
TACTCGG	60	3.7237827E-5	21.583334	15
TACCAAC	45	0.0038244321	20.555555	7
CCGTATA	45	0.0038244321	20.555555	2
TCTAATG	45	0.0038244321	20.555555	3
GAGTATA	55	5.1405135E-4	20.181818	1
GCGCTAC	55	5.1405135E-4	20.181818	1
TCACGTT	55	5.1405135E-4	20.181818	24
CGGACCA	55	5.1405135E-4	20.181818	9
GTATCAA	3495	0.0	19.373388	2
GTATTGG	245	0.0	18.87755	1
TAGACGT	50	0.0070323767	18.5	16
TACGGAC	50	0.0070323767	18.5	30
ATCACGT	60	9.2322193E-4	18.5	23
>>END_MODULE