FastQCFastQC Report
Wed 25 May 2016
SRR2088213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186104
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT341331.5613621309873638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT321881.472391066481741No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT268911.2300878640723405No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG144550.6612219729710938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112790.5159406871768224No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98180.4491094659723417No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82460.37720071872152466No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81230.3715742709404493No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT80590.36864668835517433No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67810.31018652360546434No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT63790.29179764549170584No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG63480.2903795976769632No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT59200.2708013891379367No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA52860.24180002415255636No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52430.2398330546030747No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT44630.20415314184503575No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43700.199898998400808No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43450.19875541145343498No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40860.18690785067865023No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38330.17533475077123503No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35520.16248083348276202No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.16110852914591436No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30710.14047822061530468No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29840.13649853803844647No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC28190.1289508641857844No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA27630.12638922942366876No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC27280.12478820769734653No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27100.12396482509523792No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC26450.12099149903206802No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25990.1188872990489016No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC25170.11513633386151803No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24830.11358105561309069No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22920.10484405133516063No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA22800.10429512960042157No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA22450.10269410787409931No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA22420.10255687744041456No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG22120.1011845731035669No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT22050.10086436875830244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.031.5195061
GTACGTT400.00193174223.1256
ATTGGAC5750.020.2695663
GTTATCA2300.020.1086961
GTATTGG5950.019.8991581
TGCGACG851.2463606E-619.58823422
TTGGACC12250.019.1795944
GTATCAA119350.018.5077512
GGACCCT12500.018.56
TAGGACG500.007036861518.54
TGTACCG701.2198363E-418.55
TATTGGA6800.018.227942
ACCGTGT1156.412665E-817.6956528
TGGACCC13400.017.5335835
GACCCTC12850.017.1322967
ACGTGCG650.001580550917.0769219
CGAATTA1107.8155244E-716.81818215
GTATACT1554.0199666E-1016.7096774
GTACTAG2050.016.2439041
TATACCG1059.35073E-615.8571445
Produced by FastQC (version 0.11.5)