##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088213_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2186104
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.391846408039143	31.0	30.0	33.0	27.0	34.0
2	30.781972403874654	31.0	31.0	34.0	27.0	34.0
3	30.969404017375204	31.0	31.0	34.0	27.0	34.0
4	35.02120576148253	35.0	35.0	37.0	32.0	37.0
5	34.86375991261166	35.0	35.0	37.0	32.0	37.0
6	34.91448073833633	35.0	35.0	37.0	32.0	37.0
7	34.71255301669088	35.0	35.0	37.0	31.0	37.0
8	34.84498541697925	35.0	35.0	37.0	32.0	37.0
9	36.28479523389555	38.0	35.0	39.0	32.0	39.0
10	36.15487323567406	38.0	35.0	39.0	31.0	39.0
11	36.2103317134043	38.0	35.0	39.0	32.0	39.0
12	36.03304966277908	38.0	35.0	39.0	30.0	39.0
13	36.11191828019161	38.0	35.0	39.0	31.0	39.0
14	36.96216648430267	39.0	36.0	40.0	31.0	41.0
15	37.03627595027501	39.0	36.0	40.0	31.0	41.0
16	36.97981660524842	38.0	36.0	40.0	31.0	41.0
17	37.11302984670446	39.0	36.0	40.0	31.0	41.0
18	37.136573099907416	39.0	36.0	40.0	31.0	41.0
19	37.260332994221685	39.0	36.0	40.0	31.0	41.0
20	37.22503458206929	39.0	36.0	40.0	31.0	41.0
21	37.11251843462159	39.0	36.0	40.0	31.0	41.0
22	37.068788584623604	39.0	36.0	40.0	31.0	41.0
23	36.94217018037568	39.0	35.0	40.0	31.0	41.0
24	36.87821576649601	39.0	35.0	40.0	31.0	41.0
25	36.7782772457303	38.0	35.0	40.0	30.0	41.0
26	36.47819728613094	38.0	35.0	40.0	30.0	41.0
27	36.30722463341177	38.0	35.0	40.0	30.0	41.0
28	36.12024908238583	38.0	35.0	40.0	30.0	41.0
29	35.9938003864409	38.0	34.0	40.0	30.0	41.0
30	35.81202998576463	38.0	34.0	40.0	29.0	41.0
31	35.5126709433769	38.0	34.0	40.0	27.0	41.0
32	35.19551906039237	37.0	34.0	40.0	27.0	41.0
33	35.28736327274457	38.0	34.0	40.0	27.0	41.0
34	35.23911259482623	38.0	34.0	40.0	27.0	41.0
35	35.11642995941639	38.0	34.0	40.0	26.0	41.0
36	34.999089247355116	38.0	33.0	40.0	25.0	41.0
37	34.83210634077793	38.0	33.0	40.0	25.0	41.0
38	34.6747748506018	38.0	33.0	40.0	24.0	41.0
39	34.57344252606463	38.0	33.0	40.0	24.0	41.0
40	34.35292602730703	38.0	33.0	40.0	23.0	41.0
41	34.18178641089354	38.0	33.0	40.0	21.0	41.0
42	33.94598518643212	38.0	33.0	40.0	19.0	41.0
43	33.5827655042944	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	5.0
15	13.0
16	29.0
17	100.0
18	211.0
19	530.0
20	1117.0
21	2067.0
22	3795.0
23	6485.0
24	10511.0
25	16263.0
26	23761.0
27	34395.0
28	47466.0
29	62652.0
30	79548.0
31	95335.0
32	114040.0
33	137436.0
34	163173.0
35	192895.0
36	235338.0
37	297091.0
38	345073.0
39	316774.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.27480119884507	24.86688647932578	14.684571273827778	21.173741048001375
2	17.42606939102623	23.87718059159125	36.22128681892536	22.475463198457163
3	20.43635618433524	26.051596813326356	32.92899148439415	20.583055517944253
4	13.300144915337972	16.16999923150957	34.955610529050766	35.57424532410169
5	10.666281201626273	41.49390879848352	34.44589095486765	13.393919045022562
6	31.302399153928633	35.73892184452341	15.38293695084955	17.575742050698413
7	23.1786319406579	36.64757028942813	21.471942780398372	18.701854989515596
8	27.590407409711524	33.88832370280645	20.58163747012951	17.939631417352516
9	24.81423573626872	13.86233225866656	25.112757673010982	36.21067433205374
10	16.35494011263874	28.534186845639546	33.74807419958062	21.362798842141085
11	30.417811778396636	22.02392932815639	25.72819042460926	21.830068468837712
12	19.53552987415054	28.1707091702865	32.34475578472021	19.94900517084274
13	30.739891606254783	21.523358449552262	30.113983598218564	17.62276634597439
14	20.2835729681662	23.34353717846909	29.81436381800683	26.558526035357875
15	22.523265132857357	29.348237778257573	28.282597717217477	19.84589937166759
16	20.88971979375181	28.81614964338385	28.560992523685975	21.733138039178375
17	18.13244017667961	27.652710026604403	29.24613833559611	24.968711461119874
18	19.24876401122728	26.855263976462236	34.3716035467663	19.524368465544185
19	18.467236691392543	25.204336115756615	36.0227143813835	20.30571281146734
20	23.333061922031156	23.964504890892655	34.60585589706619	18.09657729000999
21	22.587168771476563	25.009423156446353	32.98191668831858	19.421491383758504
22	20.54335017913146	24.711953319695677	34.04000907550602	20.704687425666847
23	19.532922495910533	27.142258556774973	35.16895810995268	18.15586083736181
24	18.22607707593051	25.981289087801862	33.38830174593706	22.404332090330563
25	19.40676198387634	27.107127565751675	34.66564262267486	18.820467827697126
26	20.996896762459606	25.910157979675258	33.030404774887195	20.062540482977937
27	19.611601277889797	27.400709206881285	32.57242107420324	20.415268441025678
28	19.107965586266708	27.090293965886342	32.773051968250364	21.028688479596582
29	18.199134167450403	26.684640803914178	33.947332789290904	21.168892239344515
30	17.53493886841614	26.667166795358316	36.3992746914145	19.398619644811042
31	19.111625064498302	26.13882962567197	34.88928248610313	19.860262823726593
32	20.267059572646133	26.66959119968675	32.61189769562656	20.45145153204056
33	21.4752820542847	26.528472570380917	31.853653806040334	20.142591569294048
34	19.388144388373103	29.517397159513	31.970482648583964	19.123975803529934
35	17.91964151751243	30.247188605848578	32.00442430918199	19.82874556745699
36	19.009159674013677	26.18370397748689	34.68471765295704	20.12241869554239
37	19.00824480445578	26.532315022524088	33.682523795757206	20.77691637726293
38	19.436952679286986	26.08110135656858	34.206515335043534	20.275430629100903
39	20.619147122003344	25.4152592923301	34.03054017558176	19.93505341008479
40	18.75089199781895	26.212796829428058	33.115807848117015	21.920503324635973
41	19.58150206943494	25.739809267994566	32.85872950234756	21.819959160222936
42	16.888080347504054	27.59306053142943	33.217175395132166	22.301683725934357
43	17.593444776643747	27.432683897929834	32.85049567632647	22.123375649099952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	318.0
1	383.5
2	449.0
3	1147.0
4	1845.0
5	1845.0
6	3249.5
7	4654.0
8	5269.5
9	5885.0
10	8920.0
11	11955.0
12	11955.0
13	22005.0
14	32055.0
15	52337.5
16	72620.0
17	70475.5
18	68331.0
19	68331.0
20	73168.5
21	78006.0
22	62995.5
23	47985.0
24	51122.0
25	54259.0
26	54259.0
27	57839.5
28	61420.0
29	63026.5
30	64633.0
31	68063.0
32	71493.0
33	71493.0
34	76317.5
35	81142.0
36	86621.0
37	92100.0
38	94742.5
39	97385.0
40	97385.0
41	102449.5
42	107514.0
43	124671.0
44	141828.0
45	185583.5
46	229339.0
47	229339.0
48	229908.0
49	230477.0
50	205616.5
51	180756.0
52	164742.5
53	148729.0
54	148729.0
55	127070.5
56	105412.0
57	82950.5
58	60489.0
59	51367.5
60	42246.0
61	42246.0
62	36395.5
63	30545.0
64	26093.5
65	21642.0
66	18311.0
67	14980.0
68	14980.0
69	12510.5
70	10041.0
71	8331.5
72	6622.0
73	5312.0
74	4002.0
75	4002.0
76	3134.5
77	2267.0
78	1778.5
79	1290.0
80	1006.5
81	723.0
82	723.0
83	515.0
84	307.0
85	245.0
86	183.0
87	148.5
88	114.0
89	114.0
90	84.0
91	54.0
92	30.5
93	7.0
94	4.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2186104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.980799415766874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18413784802924	28.137953984540943
2	13.921233870485327	10.853216502507369
3	5.522875984435204	6.458583628422741
4	2.7376168281742452	4.268579698251528
5	1.5034772368423563	2.9303372297761663
6	0.9159037044792275	2.142159515307756
7	0.5967580380889321	1.6283473767743866
8	0.41106502038438963	1.2818914485153612
9	0.30974443631055515	1.0866677167774377
>10	1.635327748498872	11.976515797404206
>50	0.15148101042498174	4.141188532226455
>100	0.0894456988364473	6.755944529300752
>500	0.00951480682286387	2.4246782378339113
>1k	0.00963374190814967	7.144638798440367
>5k	0.0011893508528579837	3.2547407470631193
>10k+	5.946754264289919E-4	5.514556256857539
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34133	1.5613621309873638	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32188	1.472391066481741	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26891	1.2300878640723405	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14455	0.6612219729710938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11279	0.5159406871768224	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9818	0.4491094659723417	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8246	0.37720071872152466	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8123	0.3715742709404493	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8059	0.36864668835517433	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6781	0.31018652360546434	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6379	0.29179764549170584	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6348	0.2903795976769632	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5920	0.2708013891379367	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5286	0.24180002415255636	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5243	0.2398330546030747	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4463	0.20415314184503575	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4370	0.199898998400808	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4345	0.19875541145343498	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4086	0.18690785067865023	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3833	0.17533475077123503	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3552	0.16248083348276202	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3522	0.16110852914591436	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3071	0.14047822061530468	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2984	0.13649853803844647	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2819	0.1289508641857844	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2763	0.12638922942366876	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2728	0.12478820769734653	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2710	0.12396482509523792	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2645	0.12099149903206802	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2599	0.1188872990489016	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2517	0.11513633386151803	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2483	0.11358105561309069	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2292	0.10484405133516063	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2280	0.10429512960042157	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2245	0.10269410787409931	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2242	0.10255687744041456	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2212	0.1011845731035669	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	2205	0.10086436875830244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.574347789492174E-5	0.0
2	0.0	0.0	0.0	4.574347789492174E-5	0.0
3	0.0	0.0	0.0	4.574347789492174E-5	0.0
4	0.0	0.0	0.0	9.148695578984348E-5	0.0
5	0.0	0.0	0.0	9.148695578984348E-5	0.0
6	4.574347789492174E-5	0.0	0.0	9.148695578984348E-5	0.0
7	4.574347789492174E-5	0.0	0.0	9.148695578984348E-5	0.0
8	4.574347789492174E-5	0.0	0.0	9.148695578984348E-5	0.0
9	4.574347789492174E-5	4.574347789492174E-5	0.0	2.7446086736953045E-4	0.0
10	4.574347789492174E-5	4.574347789492174E-5	0.0	4.574347789492174E-4	0.0
11	4.574347789492174E-5	4.574347789492174E-5	0.0	0.0010063565136882782	0.0
12	4.574347789492174E-5	4.574347789492174E-5	0.0	0.001235073903162887	0.0
13	4.574347789492174E-5	4.574347789492174E-5	0.0	0.0014637912926374957	0.0
14	1.3723043368476522E-4	4.574347789492174E-5	0.0	0.0016925086821121046	0.0
15	1.3723043368476522E-4	4.574347789492174E-5	0.0	0.002424404328430852	0.0
16	1.3723043368476522E-4	4.574347789492174E-5	0.0	0.004254143444227722	0.0
17	1.3723043368476522E-4	4.574347789492174E-5	0.0	0.006587060816868731	0.0
18	1.3723043368476522E-4	9.148695578984348E-5	0.0	0.007776391242136696	0.0
19	1.3723043368476522E-4	1.3723043368476522E-4	0.0	0.00891997818950974	4.574347789492174E-5
20	1.3723043368476522E-4	1.3723043368476522E-4	0.0	0.01152735642952028	4.574347789492174E-5
21	1.3723043368476522E-4	1.3723043368476522E-4	0.0	0.015324065094798783	4.574347789492174E-5
22	1.3723043368476522E-4	1.3723043368476522E-4	0.0	0.024472760673783133	4.574347789492174E-5
23	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.0382872909980495	4.574347789492174E-5
24	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.05864313866128967	4.574347789492174E-5
25	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.06742588641711465	4.574347789492174E-5
26	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.0820637993434896	4.574347789492174E-5
27	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.19052158543234907	4.574347789492174E-5
28	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.292255080270655	4.574347789492174E-5
29	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.4008043533153043	4.574347789492174E-5
30	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.6290643080109638	4.574347789492174E-5
31	1.3723043368476522E-4	1.8297391157968696E-4	0.0	0.9533398228080641	4.574347789492174E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6920	0.0	31.519506	1
GTACGTT	40	0.001931742	23.125	6
ATTGGAC	575	0.0	20.269566	3
GTTATCA	230	0.0	20.108696	1
GTATTGG	595	0.0	19.899158	1
TGCGACG	85	1.2463606E-6	19.588234	22
TTGGACC	1225	0.0	19.179594	4
GTATCAA	11935	0.0	18.507751	2
GGACCCT	1250	0.0	18.5	6
TAGGACG	50	0.0070368615	18.5	4
TGTACCG	70	1.2198363E-4	18.5	5
TATTGGA	680	0.0	18.22794	2
ACCGTGT	115	6.412665E-8	17.695652	8
TGGACCC	1340	0.0	17.533583	5
GACCCTC	1285	0.0	17.132296	7
ACGTGCG	65	0.0015805509	17.076921	9
CGAATTA	110	7.8155244E-7	16.818182	15
GTATACT	155	4.0199666E-10	16.709677	4
GTACTAG	205	0.0	16.243904	1
TATACCG	105	9.35073E-6	15.857144	5
>>END_MODULE