FastQCFastQC Report
Wed 25 May 2016
SRR2088212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025315
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT307571.518627966513851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT292071.4420966615069755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252151.2449915198376549No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG139060.6866092435003938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96560.47676534267509No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88730.4381046898877459No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85910.4241809298800433No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT71790.3544633797705542No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA67780.3346639905397432No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT62700.3095814725116834No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60670.29955834030755707No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG55560.27432769717303235No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT55440.27373519674717267No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49820.24598642680274427No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48120.23759267076973212No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47330.23369204296615587No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT45080.22258265998128685No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37320.1842676324423608No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.17518262591251235No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33530.16555449399229255No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC32380.15987636491113727No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32010.15804948859806994No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.14254572745474162No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28490.14066947610618596No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26680.13173259468280243No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA24610.12151196233672293No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24310.12003071127207374No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23510.11608070843300917No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG23070.11390820687152368No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC22300.11010632913892407No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC22070.10897070332269301No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21750.1073907021870672No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC21570.10650195154827768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61100.031.4590841
GTATTGG5150.022.9902921
ATTGGAC5300.021.9905643
CGAATTA1251.8189894E-1120.7215
TATACCG1551.8189894E-1219.0967735
GTATCAA106900.017.9981292
TTGGACC10800.017.986114
CGTCTGT6550.017.79389434
CCGAATT1502.5102054E-1017.26666614
TATTGGA7300.017.2328762
ACGTGAG650.001580495617.07692511
TTTTTAC15400.016.577921
GGACCCT11200.016.5178596
ACGTTTA1606.311893E-1016.187526
TGGACCC12150.015.9876555
GACCCTC11300.015.8805317
TATAGAC957.063922E-515.5789483
ACCGACG3150.015.2698418
CTAATAC1954.1836756E-1115.1794873
GTATAGA2109.094947E-1214.976191
Produced by FastQC (version 0.11.5)