##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088212_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2025315
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15261971594542	31.0	31.0	33.0	30.0	34.0
2	31.31834159130802	31.0	31.0	34.0	30.0	34.0
3	31.35745698817221	31.0	31.0	34.0	28.0	34.0
4	35.3069339831088	37.0	35.0	37.0	33.0	37.0
5	35.192868763624425	37.0	35.0	37.0	32.0	37.0
6	35.24545268266912	37.0	35.0	37.0	32.0	37.0
7	35.07624492980104	37.0	35.0	37.0	32.0	37.0
8	35.139776775464554	37.0	35.0	37.0	32.0	37.0
9	36.62978944016116	39.0	35.0	39.0	32.0	39.0
10	36.493734554871715	38.0	35.0	39.0	32.0	39.0
11	36.53164322586857	38.0	35.0	39.0	32.0	39.0
12	36.376652520719	38.0	35.0	39.0	32.0	39.0
13	36.41443429787465	38.0	35.0	39.0	32.0	39.0
14	37.3247657771754	39.0	36.0	41.0	32.0	41.0
15	37.380322567106845	39.0	36.0	41.0	32.0	41.0
16	37.34799426262088	39.0	36.0	41.0	32.0	41.0
17	37.466379303960125	39.0	36.0	41.0	32.0	41.0
18	37.494276692761375	39.0	36.0	41.0	32.0	41.0
19	37.60062410044857	39.0	36.0	41.0	32.0	41.0
20	37.574040087591314	39.0	36.0	41.0	32.0	41.0
21	37.448530722381456	39.0	36.0	41.0	32.0	41.0
22	37.41007695099281	39.0	36.0	41.0	32.0	41.0
23	37.2861243806519	39.0	36.0	41.0	31.0	41.0
24	37.19016399918038	39.0	36.0	41.0	31.0	41.0
25	37.11087855469396	39.0	36.0	40.0	31.0	41.0
26	36.84186854884302	39.0	35.0	40.0	31.0	41.0
27	36.68073460177799	38.0	35.0	40.0	30.0	41.0
28	36.54342559058714	38.0	35.0	40.0	30.0	41.0
29	36.38663022789048	38.0	35.0	40.0	30.0	41.0
30	36.18802704764444	38.0	35.0	40.0	30.0	41.0
31	35.90687572056692	38.0	35.0	40.0	30.0	41.0
32	35.60947210680808	38.0	34.0	40.0	29.0	41.0
33	35.72948849931986	38.0	34.0	40.0	29.0	41.0
34	35.70017799700293	38.0	34.0	40.0	29.0	41.0
35	35.58909749841383	38.0	34.0	40.0	28.0	41.0
36	35.48884346385624	38.0	34.0	40.0	27.0	41.0
37	35.33809555550618	38.0	34.0	40.0	27.0	41.0
38	35.196281566077374	38.0	34.0	40.0	26.0	41.0
39	35.10617706381476	38.0	34.0	40.0	25.0	41.0
40	34.90422921866475	38.0	34.0	40.0	24.0	41.0
41	34.73876014348385	38.0	33.0	40.0	23.0	41.0
42	34.46843379918679	38.0	33.0	40.0	22.0	41.0
43	34.136085004061094	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	6.0
15	5.0
16	16.0
17	56.0
18	124.0
19	358.0
20	666.0
21	1428.0
22	2450.0
23	4268.0
24	7009.0
25	11175.0
26	17494.0
27	25558.0
28	36216.0
29	48955.0
30	63174.0
31	76919.0
32	94377.0
33	116101.0
34	142080.0
35	172606.0
36	217148.0
37	289348.0
38	349120.0
39	348656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.44353594379146	23.08569284284173	14.445506007707444	22.025265205659366
2	17.21722299987903	23.298696745938287	36.810027082206965	22.674053171975718
3	19.722907300839623	26.036937464048805	33.172617592818895	21.06753764229268
4	12.751349790032663	15.896885175886219	35.407973574481005	35.943791459600114
5	10.996659778849216	40.80975058200823	34.63959927221198	13.553990366930577
6	31.15949864588965	36.043775906463935	15.444116100458446	17.35260934718797
7	24.10168294808462	36.05765029143615	21.20489899102115	18.635767769458084
8	27.9049925567134	33.309386441121504	20.229939540269047	18.55568146189605
9	25.099058664948416	13.755045511438963	24.603728308929725	36.5421675146829
10	16.394783033750304	28.290611583877073	33.752873009877476	21.561732372495143
11	30.715567701814283	21.958510157679175	25.77549665113822	21.550425489368322
12	20.46012595571553	28.019937639330177	31.634782737500096	19.885153667454198
13	31.206750554851958	21.11765330331331	29.35824797624073	18.317348165593994
14	20.96873819628058	23.131414125703902	29.099473415246518	26.800374262769
15	22.84128641717461	29.313810444301257	27.384332807489205	20.460570331034926
16	21.603355527411786	28.482532346820122	27.934123827651504	21.97998829811659
17	18.956261124812684	27.53438354033817	28.310262848001422	25.199092486847725
18	19.964252474306466	26.623068510330494	33.45568467127335	19.956994344089686
19	19.145219385626433	25.01921923256382	35.14258275873136	20.692978623078385
20	23.856437146814198	23.780202092020254	33.497702826473905	18.86565793469164
21	23.298844871044754	24.88225288411926	32.14053122600682	19.678371018829168
22	21.16080708433009	24.745484035816652	33.078508775178186	21.015200104675074
23	20.396382784900126	26.799584262201186	34.13419640895367	18.669836543945014
24	18.788287254081464	25.962084910248528	32.32563823405248	22.923989601617524
25	20.1338557212088	26.879226194443827	33.753712385480775	19.233205698866595
26	21.476264186064885	25.871580470198463	32.26145068791768	20.390704655818972
27	20.00952938184924	27.405613447784667	31.7859197211298	20.79893744923629
28	19.902632430017057	27.022512547430892	31.730718431453873	21.344136591098177
29	18.851734174683937	26.956103124699123	32.76013854634958	21.43202415426736
30	18.21474684184929	27.138543880828415	35.14194088327001	19.50476839405228
31	19.97728748367538	26.53365032106117	33.588997267091784	19.900064928171666
32	21.088176407126795	26.72724983521082	31.36692316997603	20.81765058768636
33	21.96448453697326	26.800670512981934	30.758277107511674	20.476567842533136
34	20.045079407400824	29.780058904417338	30.793086507530926	19.381775180650912
35	18.393681970458918	30.99917790565912	31.029889177732844	19.577250946149118
36	19.66499038421184	27.036781932687013	33.402655883158914	19.89557179994223
37	19.696936032172772	27.270276475511217	32.46082708121946	20.571960411096548
38	20.029674396328474	26.94993124526308	32.94243117737241	20.077963181036036
39	21.293033429367778	26.28894764518112	32.56362590510612	19.854393020344983
40	19.175239407203325	27.142987634022365	31.921404818509714	21.760368140264603
41	20.50071223488692	26.407694605530494	31.746419692739153	21.345173466843427
42	17.5420613583566	28.36106975951889	32.22071628363983	21.87615259848468
43	18.198551830209126	28.09706144476291	31.889557920619755	21.814828804408204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	279.0
2	347.0
3	967.0
4	1587.0
5	1587.0
6	2784.5
7	3982.0
8	4325.5
9	4669.0
10	7207.5
11	9746.0
12	9746.0
13	17808.5
14	25871.0
15	42999.5
16	60128.0
17	58865.5
18	57603.0
19	57603.0
20	62151.5
21	66700.0
22	53715.0
23	40730.0
24	44492.5
25	48255.0
26	48255.0
27	51686.0
28	55117.0
29	56224.5
30	57332.0
31	61387.0
32	65442.0
33	65442.0
34	70422.5
35	75403.0
36	81166.5
37	86930.0
38	89020.0
39	91110.0
40	91110.0
41	95589.5
42	100069.0
43	114548.0
44	129027.0
45	166819.0
46	204611.0
47	204611.0
48	209772.5
49	214934.0
50	195030.5
51	175127.0
52	160273.0
53	145419.0
54	145419.0
55	124409.0
56	103399.0
57	81466.5
58	59534.0
59	51250.0
60	42966.0
61	42966.0
62	37555.0
63	32144.0
64	27783.5
65	23423.0
66	19524.0
67	15625.0
68	15625.0
69	13108.0
70	10591.0
71	8901.5
72	7212.0
73	5798.0
74	4384.0
75	4384.0
76	3350.5
77	2317.0
78	1867.5
79	1418.0
80	1149.5
81	881.0
82	881.0
83	667.5
84	454.0
85	387.5
86	321.0
87	245.5
88	170.0
89	170.0
90	133.5
91	97.0
92	59.5
93	22.0
94	14.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2025315.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.55039829711635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39671692642975	24.670809272468137
2	14.374937741694133	10.220695244269534
3	6.125867099239255	6.53331045879469
4	3.097814670310484	4.405141815207516
5	1.808962311949891	3.2154665347145532
6	1.1498623885125587	2.452683953909701
7	0.757444751549446	1.88492238239502
8	0.5119988197753993	1.4561409576535151
9	0.4007013952364402	1.2820584778979123
>10	2.05141729965211	13.46239537145981
>50	0.1816883156416419	4.517150611881327
>100	0.12022203304813588	8.21535744684331
>500	0.010484178262917289	2.5800941745189343
>1k	0.010064811132400597	6.9736290925636295
>5k	0.0012581013915500747	3.2060594702206275
>10k+	5.591561740222554E-4	4.924084735201831
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30757	1.518627966513851	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29207	1.4420966615069755	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25215	1.2449915198376549	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13906	0.6866092435003938	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9656	0.47676534267509	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8873	0.4381046898877459	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8591	0.4241809298800433	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7179	0.3544633797705542	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6778	0.3346639905397432	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6270	0.3095814725116834	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6067	0.29955834030755707	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5556	0.27432769717303235	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5544	0.27373519674717267	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4982	0.24598642680274427	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4812	0.23759267076973212	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4733	0.23369204296615587	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4508	0.22258265998128685	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3732	0.1842676324423608	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3548	0.17518262591251235	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3353	0.16555449399229255	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3238	0.15987636491113727	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3201	0.15804948859806994	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2887	0.14254572745474162	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2849	0.14066947610618596	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2668	0.13173259468280243	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2461	0.12151196233672293	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2431	0.12003071127207374	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2351	0.11608070843300917	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2307	0.11390820687152368	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2230	0.11010632913892407	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2207	0.10897070332269301	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2175	0.1073907021870672	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2157	0.10650195154827768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4812510646492028E-4	0.0
10	0.0	4.937503548830676E-5	0.0	1.4812510646492028E-4	0.0
11	0.0	4.937503548830676E-5	0.0	1.4812510646492028E-4	0.0
12	0.0	4.937503548830676E-5	0.0	1.4812510646492028E-4	0.0
13	0.0	4.937503548830676E-5	0.0	1.4812510646492028E-4	0.0
14	0.0	4.937503548830676E-5	0.0	2.9625021292984055E-4	0.0
15	0.0	4.937503548830676E-5	0.0	7.406255323246014E-4	0.0
16	0.0	4.937503548830676E-5	0.0	0.0012837509226959758	0.0
17	0.0	4.937503548830676E-5	0.0	0.002221876596973804	0.0
18	0.0	4.937503548830676E-5	0.0	0.0025675018453919516	0.0
19	0.0	4.937503548830676E-5	0.0	0.0030612522002750188	0.0
20	0.0	4.937503548830676E-5	0.0	0.0035550025551580864	0.0
21	0.0	4.937503548830676E-5	0.0	0.004888128513342369	0.0
22	0.0	9.875007097661352E-5	0.0	0.0068631299328746394	0.0
23	0.0	9.875007097661352E-5	0.0	0.010862507807427486	0.0
24	0.0	9.875007097661352E-5	0.0	0.01673813703053599	0.0
25	0.0	9.875007097661352E-5	0.0	0.021527515472901746	0.0
26	0.0	9.875007097661352E-5	0.0	0.029180645973589294	0.0
27	0.0	9.875007097661352E-5	0.0	0.0899119396242066	0.0
28	0.0	9.875007097661352E-5	0.0	0.15286510987179772	0.0
29	0.0	9.875007097661352E-5	0.0	0.22905078963025505	0.0
30	0.0	9.875007097661352E-5	0.0	0.3809777738277749	0.0
31	0.0	9.875007097661352E-5	0.0	0.5960554284148392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6110	0.0	31.459084	1
GTATTGG	515	0.0	22.990292	1
ATTGGAC	530	0.0	21.990564	3
CGAATTA	125	1.8189894E-11	20.72	15
TATACCG	155	1.8189894E-12	19.096773	5
GTATCAA	10690	0.0	17.998129	2
TTGGACC	1080	0.0	17.98611	4
CGTCTGT	655	0.0	17.793894	34
CCGAATT	150	2.5102054E-10	17.266666	14
TATTGGA	730	0.0	17.232876	2
ACGTGAG	65	0.0015804956	17.076925	11
TTTTTAC	1540	0.0	16.57792	1
GGACCCT	1120	0.0	16.517859	6
ACGTTTA	160	6.311893E-10	16.1875	26
TGGACCC	1215	0.0	15.987655	5
GACCCTC	1130	0.0	15.880531	7
TATAGAC	95	7.063922E-5	15.578948	3
ACCGACG	315	0.0	15.269841	8
CTAATAC	195	4.1836756E-11	15.179487	3
GTATAGA	210	9.094947E-12	14.97619	1
>>END_MODULE