FastQCFastQC Report
Wed 25 May 2016
SRR2088211_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088211_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040549
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308131.5100347994583811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT292271.4323106183679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT249031.220406861094735No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG118270.5795989216627486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.4851635515736206No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT80630.395138759226071No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74210.36367663800281197No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT67400.330303266424869No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA63640.3118768527489416No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA58380.28609947617038356No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT56630.27752335278398116No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG51800.2538532522375106No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT50350.24674732143163433No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44200.21660837353084883No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42910.2102865454345865No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT41360.20269055043520148No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38090.18666545130746678No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37510.18382307898511624No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36570.1792164755661344No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32270.15814371524525997No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31080.15231195134250636No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC30810.15098877802003285No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG26290.12883787647343925No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26030.12756370957031662No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24590.12050678518379122No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC24510.12011473382898426No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC23790.11658627163572156No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23410.11472402770038848No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.1138419121520728No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC22060.10810816108802093No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21880.10722604553970526No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA21770.10668697492684567No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC20590.10090421744344291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60250.031.1352671
CGCACTA451.3237039E-424.66666629
TATTCGG450.003826810520.5555555
ACGTTTA1001.2913006E-820.3526
ATTGGAC4550.019.5164833
GTATTGG5100.018.51
GTATCAA105200.017.866922
GCGCACT650.001580501417.07692328
CGAATTA1452.9831426E-916.58620615
CAATGCG904.448677E-516.44444519
TTTTTAC17050.016.3841651
CGTCTGT7050.016.26950334
ACGATCT2650.016.05660432
CGATCTG2550.015.96078433
TGGACCC10250.015.8829265
GGACCCT10400.015.6538466
TTGGACC10650.015.6338024
GTTATCA2250.015.6222221
TGCGACG957.0639624E-515.57894822
ACCGGGG855.3666835E-415.2352938
Produced by FastQC (version 0.11.5)