##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088211_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2040549
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011970307990644	31.0	31.0	33.0	28.0	34.0
2	31.16873204221021	31.0	31.0	34.0	28.0	34.0
3	31.20807635592186	31.0	31.0	34.0	28.0	34.0
4	35.17293091222019	35.0	35.0	37.0	33.0	37.0
5	35.03560071333744	35.0	35.0	37.0	32.0	37.0
6	35.10213966927528	37.0	35.0	37.0	32.0	37.0
7	34.93802501189631	36.0	35.0	37.0	32.0	37.0
8	35.001378060512145	37.0	35.0	37.0	32.0	37.0
9	36.450748793584474	38.0	35.0	39.0	32.0	39.0
10	36.304567055238564	38.0	35.0	39.0	32.0	39.0
11	36.350225356019386	38.0	35.0	39.0	32.0	39.0
12	36.19983788676479	38.0	35.0	39.0	32.0	39.0
13	36.2436466852793	38.0	35.0	39.0	32.0	39.0
14	37.14253811106717	39.0	36.0	41.0	31.0	41.0
15	37.19778745817915	39.0	36.0	41.0	32.0	41.0
16	37.16117329208953	39.0	36.0	40.0	31.0	41.0
17	37.27688381901145	39.0	36.0	40.0	32.0	41.0
18	37.30070632952211	39.0	36.0	40.0	32.0	41.0
19	37.40608924363002	39.0	36.0	41.0	32.0	41.0
20	37.36762900572346	39.0	36.0	41.0	32.0	41.0
21	37.25248842345859	39.0	36.0	41.0	31.0	41.0
22	37.21084668880777	39.0	36.0	40.0	31.0	41.0
23	37.099798632622885	39.0	36.0	40.0	31.0	41.0
24	36.99707627702153	39.0	36.0	40.0	31.0	41.0
25	36.923362291226525	39.0	35.0	40.0	31.0	41.0
26	36.63984447322755	38.0	35.0	40.0	30.0	41.0
27	36.4570049530788	38.0	35.0	40.0	30.0	41.0
28	36.306491047262284	38.0	35.0	40.0	30.0	41.0
29	36.15578209589674	38.0	35.0	40.0	30.0	41.0
30	35.962887438625586	38.0	35.0	40.0	30.0	41.0
31	35.681764564340284	38.0	34.0	40.0	29.0	41.0
32	35.380550528313705	38.0	34.0	40.0	27.0	41.0
33	35.491436863314725	38.0	34.0	40.0	28.0	41.0
34	35.450183749569355	38.0	34.0	40.0	27.0	41.0
35	35.34984751652619	38.0	34.0	40.0	27.0	41.0
36	35.24106208672274	38.0	34.0	40.0	26.0	41.0
37	35.083107046191984	38.0	34.0	40.0	25.0	41.0
38	34.939461390047484	38.0	33.0	40.0	25.0	41.0
39	34.84375087292685	38.0	33.0	40.0	24.0	41.0
40	34.64290198373085	38.0	33.0	40.0	24.0	41.0
41	34.47429000724805	38.0	33.0	40.0	23.0	41.0
42	34.224347957338935	38.0	33.0	40.0	21.0	41.0
43	33.884151765039704	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	6.0
16	14.0
17	62.0
18	180.0
19	392.0
20	828.0
21	1681.0
22	2939.0
23	5175.0
24	8518.0
25	13221.0
26	20112.0
27	28811.0
28	40257.0
29	53854.0
30	68041.0
31	83163.0
32	100789.0
33	122277.0
34	147729.0
35	176184.0
36	220326.0
37	285986.0
38	343680.0
39	316320.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94454433586255	23.4686841629385	14.815669704574603	21.77110179662434
2	17.48475532810043	23.73748437307803	36.24799012422637	22.52977017459517
3	19.986288003865628	26.117187090336962	32.94765281304198	20.94887209275543
4	13.229625948703019	16.42396237483148	35.272811385563394	35.07360029090211
5	10.862958938991419	41.06051851732058	34.65621261729074	13.420309926397259
6	31.09663134774024	36.32130372757528	15.428641997815292	17.15342292686919
7	23.649321824665815	36.26293708212839	21.597521059283554	18.490220033922245
8	27.48363308109729	33.81055784497211	20.448369531924985	18.257439542005606
9	25.000526819008023	13.882391454456618	24.74931991341546	36.367761813119905
10	16.33281043483886	28.54099558501168	34.0545608069201	21.071633173229362
11	30.640479596422338	22.33550872828832	25.45138587703603	21.572625798253313
12	19.96663642970593	28.487823620015984	31.786935770716607	19.75860417956148
13	30.701247556417417	21.623739493636272	29.553174170284564	18.121838779661747
14	20.645179312038085	23.408749312072388	29.43766603987456	26.508405336014963
15	22.66605702680994	29.656087650921393	27.55101690770474	20.126838414563924
16	21.181799603930116	28.59117815842697	28.29444428925745	21.93257794838546
17	18.6029838048486	28.022899719634275	28.809501756635104	24.564614718882027
18	19.455891527231152	27.02204161723144	33.77767453758768	19.74439231794973
19	18.835470258249128	25.62810302521527	35.42992596600229	20.106500750533314
20	23.26746380508383	24.458711846664794	33.92636001389822	18.347464334353155
21	22.623176409877928	25.384051056847934	32.423284125987664	19.56948840728647
22	20.703594963904322	25.216694134764712	33.29540236475576	20.784308536575207
23	19.901604911227324	27.34293565114094	34.25053747790423	18.504921959727504
24	18.37123244773833	26.491008057145404	32.70761937106141	22.43014012405485
25	19.654955602634388	27.415171113264126	34.05593298666192	18.87394029743956
26	20.994350049913038	26.192853001814708	32.598972139360534	20.21382480891172
27	19.724985775886783	27.77218287823522	32.118611216883295	20.3842201289947
28	19.516071410194023	27.498531032579958	32.06637037385527	20.91902718337075
29	18.450279802151286	27.25158768547092	33.145295702284045	21.152836810093753
30	17.900427777034515	27.304808656885964	35.43007298526034	19.364690580819182
31	19.437906171329384	26.732560698125845	33.97938495963586	19.850148170908906
32	20.462140335762584	27.16386619483286	31.993840873215984	20.380152596188577
33	21.505487003742623	27.0788400572591	31.23296720637436	20.182705732623916
34	19.657944994214795	29.77242889046036	31.39659964058692	19.173026474737924
35	18.130414903048152	30.564127595073682	31.688383861402002	19.617073640476164
36	19.237567929023022	26.884480598113548	33.86201458529053	20.015936887572902
37	19.22776664515285	27.156613244768934	32.95216140362226	20.66345870645596
38	19.597471072735818	26.8452264562135	33.48368502790181	20.073617443148876
39	20.775683406769453	26.20534963874918	33.18866638340957	19.830300571071806
40	18.90976398998505	26.907268583111705	32.60534297387615	21.577624453027102
41	19.68190913327737	26.25303288477758	32.44896348972752	21.616094492217535
42	17.119951542452547	27.95164438589811	32.82626391230987	22.102140159339474
43	17.794524904817283	27.63942448821371	32.57098947391119	21.99506113305782
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	217.0
1	315.0
2	413.0
3	1055.0
4	1697.0
5	1697.0
6	3011.5
7	4326.0
8	4797.0
9	5268.0
10	7883.0
11	10498.0
12	10498.0
13	19052.0
14	27606.0
15	44927.0
16	62248.0
17	60972.5
18	59697.0
19	59697.0
20	64654.5
21	69612.0
22	56541.5
23	43471.0
24	46760.5
25	50050.0
26	50050.0
27	54041.0
28	58032.0
29	59784.5
30	61537.0
31	65876.5
32	70216.0
33	70216.0
34	75549.5
35	80883.0
36	87167.5
37	93452.0
38	94697.5
39	95943.0
40	95943.0
41	100428.0
42	104913.0
43	118227.0
44	131541.0
45	167553.0
46	203565.0
47	203565.0
48	206944.0
49	210323.0
50	189283.5
51	168244.0
52	153058.0
53	137872.0
54	137872.0
55	118855.0
56	99838.0
57	79267.0
58	58696.0
59	49969.5
60	41243.0
61	41243.0
62	35653.5
63	30064.0
64	25596.5
65	21129.0
66	17731.0
67	14333.0
68	14333.0
69	11936.5
70	9540.0
71	7728.5
72	5917.0
73	4788.5
74	3660.0
75	3660.0
76	2891.5
77	2123.0
78	1607.5
79	1092.0
80	865.5
81	639.0
82	639.0
83	482.5
84	326.0
85	254.0
86	182.0
87	143.0
88	104.0
89	104.0
90	67.5
91	31.0
92	18.5
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2040549.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.80671159240005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86532251226151	26.791848101234848
2	14.083843755559155	10.649276379576989
3	5.807368031569653	6.586724648414337
4	2.9043241384723264	4.39211780296276
5	1.6904391016099616	3.1954971789541826
6	1.0293352736805963	2.334946909435588
7	0.676729624352619	1.7909445213752884
8	0.46960765361449847	1.4203456897429638
9	0.35435683412489	1.2057359965700094
>10	1.8295002513360716	12.898668137960437
>50	0.17017305398645222	4.49593250901223
>100	0.09857800910134987	7.446666034876362
>500	0.01021088016542636	2.775873634331636
>1k	0.008509066804521967	6.246420942268186
>5k	0.0011781784806261185	2.9796333603384353
>10k+	5.236348802782749E-4	4.789368152945824
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30813	1.5100347994583811	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29227	1.4323106183679	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24903	1.220406861094735	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11827	0.5795989216627486	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9900	0.4851635515736206	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8063	0.395138759226071	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7421	0.36367663800281197	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6740	0.330303266424869	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6364	0.3118768527489416	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5838	0.28609947617038356	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5663	0.27752335278398116	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5180	0.2538532522375106	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5035	0.24674732143163433	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4420	0.21660837353084883	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4291	0.2102865454345865	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4136	0.20269055043520148	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3809	0.18666545130746678	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3751	0.18382307898511624	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3657	0.1792164755661344	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3227	0.15814371524525997	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3108	0.15231195134250636	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3081	0.15098877802003285	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2629	0.12883787647343925	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2603	0.12756370957031662	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2459	0.12050678518379122	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2451	0.12011473382898426	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2379	0.11658627163572156	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2341	0.11472402770038848	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2323	0.1138419121520728	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2206	0.10810816108802093	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2188	0.10722604553970526	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2177	0.10668697492684567	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2059	0.10090421744344291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.801283870174154E-5	0.0	0.0	0.0	0.0
6	2.4503209675435383E-4	0.0	0.0	0.0	0.0
7	2.4503209675435383E-4	0.0	0.0	0.0	0.0
8	2.4503209675435383E-4	0.0	0.0	4.900641935087077E-5	0.0
9	2.4503209675435383E-4	0.0	0.0	4.900641935087077E-5	0.0
10	2.4503209675435383E-4	0.0	0.0	9.801283870174154E-5	0.0
11	2.4503209675435383E-4	0.0	0.0	1.960256774034831E-4	0.0
12	2.4503209675435383E-4	0.0	0.0	4.410577741578369E-4	0.0
13	2.4503209675435383E-4	0.0	0.0	5.390706128595785E-4	0.0
14	2.4503209675435383E-4	0.0	0.0	6.3708345156132E-4	0.0
15	2.4503209675435383E-4	0.0	0.0	9.801283870174153E-4	0.0
16	2.9403851610522463E-4	0.0	0.0	0.0015682054192278647	0.0
17	3.430449354560954E-4	0.0	0.0	0.002156282451438314	0.0
18	3.430449354560954E-4	0.0	0.0	0.0024993273868944093	0.0
19	3.430449354560954E-4	0.0	0.0	0.0031364108384557294	0.0
20	3.430449354560954E-4	0.0	0.0	0.0036754814513153076	0.0
21	3.430449354560954E-4	0.0	0.0	0.005439712547946655	0.0
22	3.430449354560954E-4	0.0	0.0	0.008380097708998903	0.0
23	3.430449354560954E-4	0.0	0.0	0.014162855192401652	0.0
24	3.430449354560954E-4	0.0	0.0	0.022738978578804036	0.0
25	3.430449354560954E-4	0.0	0.0	0.029109813094417236	0.0
26	3.430449354560954E-4	0.0	0.0	0.03964619325485445	0.0
27	3.430449354560954E-4	0.0	0.0	0.12232002269977345	0.0
28	3.430449354560954E-4	0.0	0.0	0.2078852308863938	0.0
29	3.430449354560954E-4	0.0	0.0	0.3025166266529253	0.0
30	3.430449354560954E-4	0.0	0.0	0.48462448096076105	0.0
31	3.430449354560954E-4	0.0	0.0	0.7545028323260063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6025	0.0	31.135267	1
CGCACTA	45	1.3237039E-4	24.666666	29
TATTCGG	45	0.0038268105	20.555555	5
ACGTTTA	100	1.2913006E-8	20.35	26
ATTGGAC	455	0.0	19.516483	3
GTATTGG	510	0.0	18.5	1
GTATCAA	10520	0.0	17.86692	2
GCGCACT	65	0.0015805014	17.076923	28
CGAATTA	145	2.9831426E-9	16.586206	15
CAATGCG	90	4.448677E-5	16.444445	19
TTTTTAC	1705	0.0	16.384165	1
CGTCTGT	705	0.0	16.269503	34
ACGATCT	265	0.0	16.056604	32
CGATCTG	255	0.0	15.960784	33
TGGACCC	1025	0.0	15.882926	5
GGACCCT	1040	0.0	15.653846	6
TTGGACC	1065	0.0	15.633802	4
GTTATCA	225	0.0	15.622222	1
TGCGACG	95	7.0639624E-5	15.578948	22
ACCGGGG	85	5.3666835E-4	15.235293	8
>>END_MODULE