FastQCFastQC Report
Wed 25 May 2016
SRR2088210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3282521
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT368171.1216074474466424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT363961.1087819392473042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319080.9720577568277553No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG187190.5702629168252084No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132270.4029524868233897No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA108380.330173059060399No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA106030.3230139274051864No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT100820.3071419802036301No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT95840.29197071397258384No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA82330.2508133230526172No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74050.22558880811425122No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT73930.22522323543398504No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG73820.22488812714374107No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA62380.19003686495836586No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58720.17888689821024753No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56960.17352516556634368No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT52120.158780400795608No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA45400.13830833070070228No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.13212405952619952No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC42140.12837693955347126No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39830.1213396654583474No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37400.1139368186829574No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36890.11238313479182616No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35380.1077830118984768No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34960.1065035075175452No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.10229942169448421No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.1021470997443733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89100.027.3243541
GTATTGG11750.022.0425531
ATTGGAC12050.021.6473033
TTGGACC18800.019.3856374
GGACCCT18600.018.997316
TGGACCC18850.018.6472155
TATTGGA14650.017.931742
CTAGTAC3400.016.8676473
GACCCTC20550.016.8345497
CGTCTGT8450.016.42011834
GTATCAA150250.016.3267882
GTACCGT957.06606E-515.5789486
AAGACCG1902.7284841E-1115.5789485
CGAATTA1701.4897523E-915.23529415
TAGGTCG1101.4534218E-515.13636421
CGCGAAT750.004106563514.835
TTTTTAC20750.014.81
ACCCTCG22950.014.7516358
TAGTACT4400.014.2954544
AACCGTT1203.304028E-513.8750017
Produced by FastQC (version 0.11.5)