##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088210_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3282521
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.069228803105904	31.0	31.0	33.0	30.0	34.0
2	31.221882205780254	31.0	31.0	34.0	28.0	34.0
3	31.281586012701823	31.0	31.0	34.0	28.0	34.0
4	35.23410969800346	37.0	35.0	37.0	33.0	37.0
5	35.08865838177425	35.0	35.0	37.0	32.0	37.0
6	35.148790213375634	37.0	35.0	37.0	32.0	37.0
7	34.99753360298381	37.0	35.0	37.0	32.0	37.0
8	35.055051285277386	37.0	35.0	37.0	32.0	37.0
9	36.564457927306485	38.0	35.0	39.0	32.0	39.0
10	36.396224121643094	38.0	35.0	39.0	32.0	39.0
11	36.42887707344447	38.0	35.0	39.0	32.0	39.0
12	36.27649084347061	38.0	35.0	39.0	32.0	39.0
13	36.32545808541666	38.0	35.0	39.0	32.0	39.0
14	37.274224902140766	39.0	36.0	41.0	32.0	41.0
15	37.32258712130098	39.0	36.0	41.0	32.0	41.0
16	37.29554571014169	39.0	36.0	41.0	32.0	41.0
17	37.36198031939476	39.0	36.0	41.0	32.0	41.0
18	37.35335767844288	39.0	36.0	41.0	32.0	41.0
19	37.44896194114219	39.0	36.0	41.0	32.0	41.0
20	37.405540741399676	39.0	36.0	41.0	32.0	41.0
21	37.291339187167424	39.0	36.0	41.0	31.0	41.0
22	37.24459158067839	39.0	36.0	40.0	31.0	41.0
23	37.13081073967234	39.0	36.0	40.0	31.0	41.0
24	37.04444023358876	39.0	36.0	40.0	31.0	41.0
25	36.96299703794736	39.0	36.0	40.0	31.0	41.0
26	36.714794208475745	39.0	35.0	40.0	30.0	41.0
27	36.54591577631948	38.0	35.0	40.0	30.0	41.0
28	36.39972021504204	38.0	35.0	40.0	30.0	41.0
29	36.26299907906149	38.0	35.0	40.0	30.0	41.0
30	36.0863921358005	38.0	35.0	40.0	30.0	41.0
31	35.850509410297754	38.0	34.0	40.0	29.0	41.0
32	35.59145577438804	38.0	34.0	40.0	28.0	41.0
33	35.76996400023031	38.0	34.0	40.0	29.0	41.0
34	35.768310393139906	38.0	34.0	40.0	29.0	41.0
35	35.68306219518474	38.0	34.0	40.0	28.0	41.0
36	35.61057979522446	38.0	34.0	40.0	27.0	41.0
37	35.479169516356485	38.0	34.0	40.0	27.0	41.0
38	35.36582675327896	38.0	34.0	40.0	27.0	41.0
39	35.324563955569516	38.0	34.0	40.0	26.0	41.0
40	35.180286432287865	38.0	34.0	40.0	25.0	41.0
41	35.05929131908067	38.0	34.0	40.0	25.0	41.0
42	34.863615495529196	38.0	33.0	40.0	24.0	41.0
43	34.554215190093224	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	9.0
15	18.0
16	22.0
17	102.0
18	249.0
19	489.0
20	1081.0
21	2286.0
22	4262.0
23	7423.0
24	12140.0
25	18974.0
26	28818.0
27	41540.0
28	58900.0
29	79388.0
30	101989.0
31	126436.0
32	155473.0
33	190260.0
34	229576.0
35	279901.0
36	351254.0
37	456439.0
38	588171.0
39	547317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.572383238370755	22.797081877008555	13.89998723542058	22.730547649200112
2	17.656733955395868	23.30431397087787	36.17320346160771	22.86574861211855
3	19.96514264493662	26.314378491409503	31.96625398588463	21.75422487776925
4	13.258163466433267	16.109965480799666	35.88327386176661	34.74859719100045
5	11.43224978606382	40.36775393059176	34.90302118402289	13.296975099321529
6	31.531648997828192	36.626513585137765	15.616046325370043	16.225791091664
7	24.72032928349887	34.868748745247935	21.860758849676817	18.550163121576375
8	27.338530355175184	33.86034697112372	20.476121858778665	18.325000814922433
9	25.332633058554688	13.684451676013648	23.459956539501196	37.522958725930465
10	16.094002140428042	27.674948614190132	34.34808185537884	21.88296739000299
11	31.78002516967904	21.964581490872412	25.08486617450429	21.17052716494426
12	20.018303005525325	27.222034527730365	32.0859790386718	20.673683428072508
13	30.761052252217127	21.01186862170874	28.56240675992629	19.664672366147848
14	20.9515186650748	22.339872311555663	28.49989383160077	28.208715191768764
15	22.93097287115604	29.01297508835435	26.737376546867488	21.318675493622127
16	21.81524505098368	28.369353920355728	27.211554777562736	22.603846251097863
17	19.62979063957245	27.130214856203512	28.179743556857673	25.06025094736637
18	20.658359839891354	26.52570996499337	32.127441073491994	20.688489121623288
19	20.05202708527988	25.155330308625594	33.262483316938415	21.53015928915611
20	23.90936722110841	24.219098674463925	32.157570355223925	19.713963749203735
21	23.396864787765256	25.023480428609595	30.927144106618055	20.652510677007093
22	21.884460145114076	24.685021055463164	31.677146924574128	21.75337187484863
23	21.010680510497874	26.486410901864755	32.57018005368435	19.93272853395302
24	19.922096461835277	25.798037544923552	31.121659236909682	23.158206756331488
25	20.976133892212722	26.483181676522406	32.1856889872144	20.354995444050473
26	22.165189499168473	25.67313354583261	30.927205035398096	21.23447191960082
27	20.968761509827356	26.998334511797488	30.579423558904878	21.453480419470278
28	20.78963089649693	26.36759978077825	30.843732606737323	21.9990367159875
29	20.04511166874485	26.357089566220598	31.60430656803109	21.993492197003462
30	19.46537432662274	26.558337326707125	33.27713668853908	20.69915165813105
31	20.73461220811687	26.005104003904318	32.28634333184769	20.97394045613113
32	21.54328944125567	26.19331300546135	30.501647971178247	21.76174958210473
33	22.42325334704637	26.162879079829192	30.062412395838443	21.351455177285995
34	20.87822134268143	28.50257469792272	30.213180662058214	20.406023297337626
35	19.744153959715717	29.319111743687245	30.41790136300727	20.518832933589763
36	20.669844914929712	26.108073642179285	32.14431834556428	21.077763097326716
37	20.66366064375521	26.339846721468042	31.464657804169416	21.53183483060733
38	21.023780198207415	25.889979074010494	31.929788111028078	21.156452616754013
39	21.63730254886412	25.53119995271927	32.01670301576136	20.814794482655252
40	20.115910911156394	26.185239942105472	31.26663926902524	22.432209877712893
41	20.959378477700525	25.74018566827143	31.136160286560237	22.164275567467808
42	18.83613235071459	27.268553651294237	31.46520616318982	22.430107834801362
43	19.465161075892585	26.99949215861833	31.049946062797467	22.48540070269162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	349.0
1	438.0
2	527.0
3	1398.0
4	2269.0
5	2269.0
6	3946.5
7	5624.0
8	5987.5
9	6351.0
10	9871.5
11	13392.0
12	13392.0
13	24272.0
14	35152.0
15	56179.5
16	77207.0
17	75919.5
18	74632.0
19	74632.0
20	79883.0
21	85134.0
22	70811.5
23	56489.0
24	62274.5
25	68060.0
26	68060.0
27	75960.0
28	83860.0
29	89184.0
30	94508.0
31	101688.5
32	108869.0
33	108869.0
34	118247.0
35	127625.0
36	138530.5
37	149436.0
38	154867.5
39	160299.0
40	160299.0
41	168385.5
42	176472.0
43	196871.0
44	217270.0
45	265391.5
46	313513.0
47	313513.0
48	320007.0
49	326501.0
50	297065.0
51	267629.0
52	249218.5
53	230808.0
54	230808.0
55	203001.5
56	175195.0
57	146318.5
58	117442.0
59	103523.0
60	89604.0
61	89604.0
62	79033.5
63	68463.0
64	59526.5
65	50590.0
66	42724.5
67	34859.0
68	34859.0
69	29568.0
70	24277.0
71	20356.0
72	16435.0
73	13257.5
74	10080.0
75	10080.0
76	8085.5
77	6091.0
78	4709.5
79	3328.0
80	2709.0
81	2090.0
82	2090.0
83	1547.5
84	1005.0
85	773.5
86	542.0
87	427.0
88	312.0
89	312.0
90	242.0
91	172.0
92	103.0
93	34.0
94	28.5
95	23.0
96	23.0
97	12.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3282521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.34836288521367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25430519414282	29.87597231186395
2	14.116974079689395	11.674275341763046
3	5.499526171984136	6.821892115677901
4	2.6590555642338987	4.397903776075593
5	1.4518539481783124	3.001589195280353
6	0.8777662834906826	2.177651929090685
7	0.5807610153488917	1.6809462048561783
8	0.3940359114540206	1.3034191885285413
9	0.28536059220251336	1.0619273987576097
>10	1.614447208687644	12.67108351310005
>50	0.16022076563753368	4.63851824562546
>100	0.09262654804725522	7.121758754825716
>500	0.00601365948380002	1.6870165591724928
>1k	0.005790931354625742	4.776201589853532
>5k	6.681843870722011E-4	1.9344436079378624
>10k+	5.939416773975121E-4	5.175400267590958
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36817	1.1216074474466424	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36396	1.1087819392473042	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31908	0.9720577568277553	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18719	0.5702629168252084	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13227	0.4029524868233897	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10838	0.330173059060399	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10603	0.3230139274051864	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10082	0.3071419802036301	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9584	0.29197071397258384	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8233	0.2508133230526172	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7405	0.22558880811425122	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7393	0.22522323543398504	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7382	0.22488812714374107	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6238	0.19003686495836586	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5872	0.17888689821024753	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5696	0.17352516556634368	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5212	0.158780400795608	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4540	0.13830833070070228	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4337	0.13212405952619952	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4214	0.12837693955347126	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3983	0.1213396654583474	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3740	0.1139368186829574	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3689	0.11238313479182616	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3538	0.1077830118984768	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3496	0.1065035075175452	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3358	0.10229942169448421	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3353	0.1021470997443733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.046439002218112E-5	0.0	0.0	0.0	3.046439002218112E-5
7	3.046439002218112E-5	0.0	0.0	0.0	3.046439002218112E-5
8	6.092878004436224E-5	0.0	0.0	0.0	3.046439002218112E-5
9	6.092878004436224E-5	0.0	0.0	3.046439002218112E-5	3.046439002218112E-5
10	6.092878004436224E-5	0.0	0.0	1.8278634013308673E-4	3.046439002218112E-5
11	6.092878004436224E-5	0.0	0.0	3.046439002218112E-4	3.046439002218112E-5
12	6.092878004436224E-5	0.0	0.0	4.8743024035489795E-4	3.046439002218112E-5
13	1.2185756008872449E-4	0.0	0.0	5.178946303770791E-4	3.046439002218112E-5
14	1.523219501109056E-4	0.0	0.0	9.139317006654336E-4	3.046439002218112E-5
15	1.8278634013308673E-4	0.0	0.0	0.0016755414512199618	3.046439002218112E-5
16	1.8278634013308673E-4	0.0	0.0	0.0024066868117523085	3.046439002218112E-5
17	1.8278634013308673E-4	0.0	0.0	0.003747119972728278	3.046439002218112E-5
18	1.8278634013308673E-4	0.0	0.0	0.004173621433038814	3.046439002218112E-5
19	2.1325073015526785E-4	0.0	0.0	0.0048743024035489795	3.046439002218112E-5
20	2.1325073015526785E-4	0.0	0.0	0.005788234104214413	3.046439002218112E-5
21	2.1325073015526785E-4	0.0	0.0	0.007951205795789272	3.046439002218112E-5
22	2.1325073015526785E-4	0.0	0.0	0.013221545269626607	3.046439002218112E-5
23	2.1325073015526785E-4	0.0	0.0	0.022939685686702384	3.046439002218112E-5
24	2.1325073015526785E-4	0.0	0.0	0.03567380071597409	3.046439002218112E-5
25	2.1325073015526785E-4	0.0	0.0	0.04161435677029941	3.046439002218112E-5
26	2.1325073015526785E-4	0.0	0.0	0.0488953459856007	3.046439002218112E-5
27	2.1325073015526785E-4	0.0	0.0	0.11046387822042875	3.046439002218112E-5
28	2.1325073015526785E-4	0.0	0.0	0.17483513433729747	3.046439002218112E-5
29	2.1325073015526785E-4	0.0	0.0	0.2506305367124841	3.046439002218112E-5
30	2.4371512017744897E-4	0.0	0.0	0.4000888341613047	3.046439002218112E-5
31	2.741795101996301E-4	0.0	0.0	0.6321970217403027	3.046439002218112E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8910	0.0	27.324354	1
GTATTGG	1175	0.0	22.042553	1
ATTGGAC	1205	0.0	21.647303	3
TTGGACC	1880	0.0	19.385637	4
GGACCCT	1860	0.0	18.99731	6
TGGACCC	1885	0.0	18.647215	5
TATTGGA	1465	0.0	17.93174	2
CTAGTAC	340	0.0	16.867647	3
GACCCTC	2055	0.0	16.834549	7
CGTCTGT	845	0.0	16.420118	34
GTATCAA	15025	0.0	16.326788	2
GTACCGT	95	7.06606E-5	15.578948	6
AAGACCG	190	2.7284841E-11	15.578948	5
CGAATTA	170	1.4897523E-9	15.235294	15
TAGGTCG	110	1.4534218E-5	15.136364	21
CGCGAAT	75	0.0041065635	14.8	35
TTTTTAC	2075	0.0	14.8	1
ACCCTCG	2295	0.0	14.751635	8
TAGTACT	440	0.0	14.295454	4
AACCGTT	120	3.304028E-5	13.875001	7
>>END_MODULE