FastQCFastQC Report
Wed 25 May 2016
SRR2088209_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088209_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912381
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT455361.5635316945138704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT451901.5516513807774464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT412051.4148217558073617No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG264640.9086723200020876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154120.5291890037738881No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA150900.5181327580423029No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT137110.47078318393094853No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA133910.45979561053309986No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT127300.4370994042331686No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA110110.3780755333866002No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT107330.3685300789972191No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT103470.3552763185860641No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG95890.32924950409991005No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA85810.2946386478966866No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA82410.28296435116147234No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA80110.2750670327817686No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT74730.25659417500663545No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA61610.21154512407545578No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC58190.199802155006505No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.1877501604357397No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52570.1805052292265332No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC50050.17185251517572733No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48740.16735447731598302No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT48480.16646173697740782No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG47520.1631654649580532No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37930.1302370809313754No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37600.12910398742472226No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG37580.12903531509098567No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.1267691280776794No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA36590.12563603457102626No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC35650.1224084348854082No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA34680.1190778266991853No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA34420.1181850863606101No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC33590.11533518451054309No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA33220.11406474633641683No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC33000.11330935066531474No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32960.11317200599784162No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG31980.10980706164475046No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC31910.10956670847667252No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG31200.10712884062902485No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC31010.10647645345852758No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA30410.10441628344643095No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA30360.10424460261208957No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT29620.10170372626383704No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT29270.10050196042344735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.030.6006851
GTATTGG11300.026.5221231
ATTGGAC12800.024.5703123
ACGTTTA1400.023.78571326
TATTGGA13700.021.8759122
TTGGACC21850.021.3363844
GGACCCT20750.021.1301216
TGGACCC22150.020.2957125
GCCGTCA2100.018.50000213
GACCCTC23350.018.2226987
CGGGATA2100.017.6190524
GTATCAA183350.017.1025352
TTTTTAC24600.017.0711381
AGCCGTC2450.016.61224412
CGTCTGT8700.016.37356434
ACCCTCG26800.015.8768658
GTTATCA3300.015.6969691
GCCGGAA1902.7284841E-1115.57894815
ATCACGT2350.014.95744623
CAATGCG1001.0942787E-414.819
Produced by FastQC (version 0.11.5)