##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088209_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2912381
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141825880611087	31.0	31.0	33.0	30.0	34.0
2	31.290493929194017	31.0	31.0	34.0	30.0	34.0
3	31.331588483786977	31.0	31.0	34.0	28.0	34.0
4	35.29155010968689	37.0	35.0	37.0	33.0	37.0
5	35.180610641258816	37.0	35.0	37.0	32.0	37.0
6	35.2373199110968	37.0	35.0	37.0	32.0	37.0
7	35.063610496016835	37.0	35.0	37.0	32.0	37.0
8	35.13264370286717	37.0	35.0	37.0	32.0	37.0
9	36.60250427399437	39.0	35.0	39.0	32.0	39.0
10	36.491482055404155	38.0	35.0	39.0	32.0	39.0
11	36.51672806545572	38.0	35.0	39.0	32.0	39.0
12	36.36525543876299	38.0	35.0	39.0	32.0	39.0
13	36.39904978091809	38.0	35.0	39.0	32.0	39.0
14	37.308436636552706	39.0	36.0	41.0	32.0	41.0
15	37.35511871558014	39.0	36.0	41.0	32.0	41.0
16	37.339416099747936	39.0	36.0	41.0	32.0	41.0
17	37.46025640189247	39.0	36.0	41.0	32.0	41.0
18	37.478485129521175	39.0	36.0	41.0	32.0	41.0
19	37.583228636637855	39.0	36.0	41.0	32.0	41.0
20	37.55564570706923	39.0	36.0	41.0	32.0	41.0
21	37.426280764776315	39.0	36.0	41.0	32.0	41.0
22	37.38613766536727	39.0	36.0	41.0	32.0	41.0
23	37.253484691735046	39.0	36.0	41.0	31.0	41.0
24	37.16509515753605	39.0	36.0	41.0	31.0	41.0
25	37.09064164338388	39.0	36.0	40.0	31.0	41.0
26	36.82389151694095	39.0	35.0	40.0	31.0	41.0
27	36.65867892971421	38.0	35.0	40.0	30.0	41.0
28	36.49787888329171	38.0	35.0	40.0	30.0	41.0
29	36.34877476538956	38.0	35.0	40.0	30.0	41.0
30	36.148650880499495	38.0	35.0	40.0	30.0	41.0
31	35.853423023979346	38.0	35.0	40.0	29.0	41.0
32	35.57506143598657	38.0	34.0	40.0	28.0	41.0
33	35.67296758219477	38.0	34.0	40.0	29.0	41.0
34	35.63527127803677	38.0	34.0	40.0	29.0	41.0
35	35.52139194700144	38.0	34.0	40.0	27.0	41.0
36	35.4133246989319	38.0	34.0	40.0	27.0	41.0
37	35.248218210460784	38.0	34.0	40.0	26.0	41.0
38	35.10253637831039	38.0	34.0	40.0	25.0	41.0
39	35.00015279594256	38.0	34.0	40.0	25.0	41.0
40	34.788542776511726	38.0	33.0	40.0	24.0	41.0
41	34.61551459098243	38.0	33.0	40.0	23.0	41.0
42	34.338691263265346	38.0	33.0	40.0	22.0	41.0
43	34.01199740006545	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	2.0
14	9.0
15	9.0
16	25.0
17	89.0
18	196.0
19	452.0
20	1013.0
21	1904.0
22	3676.0
23	6382.0
24	10710.0
25	17157.0
26	26028.0
27	37953.0
28	53144.0
29	72808.0
30	92425.0
31	112483.0
32	137460.0
33	167370.0
34	204534.0
35	247142.0
36	310875.0
37	414891.0
38	494012.0
39	499628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.978773038280366	22.862942726243578	14.34506680272945	21.813217432746608
2	17.25505694481594	22.970277583873813	37.20124530409998	22.573420167210266
3	19.868073579658706	26.274240904606916	33.02998474444106	20.827700771293316
4	12.39666101378906	15.165666854714408	35.18749778960925	37.25017434188727
5	10.615197668162235	41.1621968416907	34.5494974730298	13.673108017117267
6	31.32275619158345	35.54964134156898	15.362859461038921	17.76474300580865
7	23.964962001880934	36.26534440377135	20.791029745077996	18.978663849269722
8	28.51594623093613	32.71876172794699	20.115465661944643	18.64982637917223
9	25.056783435958412	13.375207433368095	24.711670622765357	36.856338507908134
10	16.35809325771594	27.783281102300833	33.39696969592921	22.461655944054023
11	30.555651887579273	21.305694550266605	26.233689891535484	21.904963670618645
12	20.677445705077734	27.37976246926484	31.946610007413177	19.996181818244246
13	31.836802945768426	20.591227590071494	29.62095275309103	17.951016711069055
14	20.990934908585107	22.45794763803225	29.122288601663037	27.428828851719604
15	22.62870139586819	28.738272911408224	28.159090448674124	20.473935244049457
16	21.462095790351608	28.397520791407445	27.66949104529936	22.470892372941588
17	18.698103029789028	26.70148582894889	28.21598547717486	26.384425664087217
18	20.222182468571248	25.988117626093565	33.94524960848186	19.84445029685333
19	18.957787459813808	23.939793591566488	35.577007266562994	21.52541168205671
20	24.78223144567967	22.62094142215596	33.83643829567629	18.760388836488083
21	23.947999935448006	23.854090519063266	32.35236049129561	19.845549054193114
22	21.54615072684515	23.45489824305268	33.401433397622085	21.59751763248009
23	20.381811308341867	26.067296826891813	34.873012837262706	18.677879027503614
24	18.697416306451665	24.868758586187727	32.73448769237267	23.69933741498794
25	20.25442412926056	26.051811215634217	34.31611454682612	19.377650108279102
26	22.116955164863388	24.705799138230883	32.478545904536524	20.698699792369197
27	20.224792017253236	26.6974684974253	31.992448790182326	21.08529069513913
28	19.92095127663585	25.93973796697616	32.08920810841713	22.050102647970853
29	18.855568691046948	25.95017616170412	33.235212013812756	21.959043133436182
30	17.96619329682483	26.186649342925943	36.06784277194502	19.77931458830421
31	19.900830282851043	25.480423062779217	34.29441408936537	20.324332565004372
32	21.45337440396706	25.686131038487066	31.512188824195736	21.348305733350134
33	22.605352802397764	25.63823208570582	30.88328759183637	20.87312752006005
34	20.10334499504014	29.581637842026847	30.981901063082063	19.333116099850947
35	18.360372492472653	30.884764047011704	31.149118195730573	19.605745264785067
36	19.81574526135145	25.96631416013221	34.05004358976384	20.167896988752503
37	19.902375410360115	26.62446980666335	32.61702366551629	20.85613111746025
38	20.317259314629506	26.00665915620243	33.36641050741644	20.30967102175162
39	21.626257004148837	25.372607498812826	33.077815024888565	19.923320472149765
40	19.27580903734779	26.55569446442619	31.918454350581193	22.25004214764483
41	20.85846597680729	25.847545358934838	31.694788559601232	21.59920010465664
42	17.633922210040513	28.11503714658213	32.29797200297626	21.953068640401103
43	18.459947376390655	27.92522681613429	31.689226100568575	21.92559970690648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	296.5
2	398.0
3	1345.0
4	2292.0
5	2292.0
6	4094.0
7	5896.0
8	6524.5
9	7153.0
10	10923.0
11	14693.0
12	14693.0
13	26858.0
14	39023.0
15	65265.0
16	91507.0
17	89620.5
18	87734.0
19	87734.0
20	91642.5
21	95551.0
22	75598.0
23	55645.0
24	59590.5
25	63536.0
26	63536.0
27	67101.5
28	70667.0
29	71874.5
30	73082.0
31	76919.5
32	80757.0
33	80757.0
34	87486.5
35	94216.0
36	102483.0
37	110750.0
38	114918.0
39	119086.0
40	119086.0
41	126620.0
42	134154.0
43	158416.0
44	182678.0
45	249038.0
46	315398.0
47	315398.0
48	321909.5
49	328421.0
50	293706.0
51	258991.0
52	239769.5
53	220548.0
54	220548.0
55	187154.0
56	153760.0
57	121152.5
58	88545.0
59	75745.0
60	62945.0
61	62945.0
62	55121.5
63	47298.0
64	41396.5
65	35495.0
66	30417.5
67	25340.0
68	25340.0
69	21610.0
70	17880.0
71	14960.5
72	12041.0
73	9687.5
74	7334.0
75	7334.0
76	5831.5
77	4329.0
78	3275.5
79	2222.0
80	1774.0
81	1326.0
82	1326.0
83	990.0
84	654.0
85	523.0
86	392.0
87	321.5
88	251.0
89	251.0
90	202.0
91	153.0
92	91.0
93	29.0
94	20.5
95	12.0
96	12.0
97	7.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2912381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.13829825274739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67415832099316	24.126954955239533
2	14.272050307039965	9.744470201198906
3	5.683638622738675	5.820892513916618
4	2.9117548816568197	3.9760942635557477
5	1.6628582088848674	2.838357474347046
6	1.0142131009899016	2.0774105600062245
7	0.6813470971907382	1.6282021292278754
8	0.48316428569514047	1.319552519210908
9	0.3481771656347369	1.0697558332711388
>10	1.9045258088534875	12.424496619655514
>50	0.20248581093756401	4.80737071143386
>100	0.137661136473218	8.700124234861772
>500	0.01188107877402403	2.7443853861695318
>1k	0.00985012513743868	7.267204609209915
>5k	0.0010154768182926474	2.412972207469583
>10k+	0.0012185721819511768	9.041755781225726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45536	1.5635316945138704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	45190	1.5516513807774464	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	41205	1.4148217558073617	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	26464	0.9086723200020876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15412	0.5291890037738881	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	15090	0.5181327580423029	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13711	0.47078318393094853	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13391	0.45979561053309986	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	12730	0.4370994042331686	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	11011	0.3780755333866002	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	10733	0.3685300789972191	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	10347	0.3552763185860641	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9589	0.32924950409991005	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8581	0.2946386478966866	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8241	0.28296435116147234	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	8011	0.2750670327817686	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	7473	0.25659417500663545	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6161	0.21154512407545578	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5819	0.199802155006505	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5468	0.1877501604357397	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5257	0.1805052292265332	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5005	0.17185251517572733	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4874	0.16735447731598302	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4848	0.16646173697740782	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4752	0.1631654649580532	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3793	0.1302370809313754	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3760	0.12910398742472226	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3758	0.12903531509098567	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3692	0.1267691280776794	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3659	0.12563603457102626	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	3565	0.1224084348854082	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3468	0.1190778266991853	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3442	0.1181850863606101	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3359	0.11533518451054309	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3322	0.11406474633641683	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	3300	0.11330935066531474	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3296	0.11317200599784162	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	3198	0.10980706164475046	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3191	0.10956670847667252	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	3120	0.10712884062902485	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	3101	0.10647645345852758	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	3041	0.10441628344643095	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	3036	0.10424460261208957	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	2962	0.10170372626383704	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	2927	0.10050196042344735	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.4336166868277196E-5	0.0	0.0	0.0	0.0
4	3.4336166868277196E-5	0.0	0.0	0.0	0.0
5	3.4336166868277196E-5	0.0	0.0	0.0	0.0
6	6.867233373655439E-5	0.0	0.0	0.0	0.0
7	6.867233373655439E-5	0.0	0.0	0.0	0.0
8	6.867233373655439E-5	0.0	0.0	3.4336166868277196E-5	0.0
9	6.867233373655439E-5	0.0	0.0	1.0300850060483158E-4	0.0
10	6.867233373655439E-5	0.0	0.0	1.0300850060483158E-4	0.0
11	6.867233373655439E-5	0.0	0.0	1.3734466747310879E-4	0.0
12	6.867233373655439E-5	0.0	0.0	4.1203400241932633E-4	0.0
13	6.867233373655439E-5	0.0	0.0	4.463701692876035E-4	0.0
14	6.867233373655439E-5	0.0	0.0	6.180510036289895E-4	0.0
15	6.867233373655439E-5	0.0	0.0	0.0011674296735214246	0.0
16	6.867233373655439E-5	0.0	0.0	0.0022318508464380174	0.0
17	6.867233373655439E-5	0.0	0.0	0.0032619358524863334	0.0
18	6.867233373655439E-5	0.0	0.0	0.003879986856115323	0.0
19	6.867233373655439E-5	0.0	0.0	0.0044293655260077575	0.0
20	6.867233373655439E-5	0.0	0.0	0.005493786698924351	0.0
21	6.867233373655439E-5	0.0	0.0	0.007450948210416151	0.0
22	6.867233373655439E-5	0.0	0.0	0.012395356239448067	0.0
23	6.867233373655439E-5	0.0	0.0	0.02115107879085875	0.0
24	6.867233373655439E-5	0.0	0.0	0.03330608186222888	0.0
25	6.867233373655439E-5	0.0	0.0	0.039349247231045666	0.0
26	6.867233373655439E-5	0.0	0.0	0.04625081677156938	0.0
27	6.867233373655439E-5	0.0	0.0	0.09813276490953622	0.0
28	6.867233373655439E-5	0.0	0.0	0.16148299278150763	0.0
29	6.867233373655439E-5	0.0	0.0	0.24231032958943216	0.0
30	6.867233373655439E-5	0.0	0.0	0.40969914307228344	0.0
31	6.867233373655439E-5	0.0	0.0	0.6375882825770391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10205	0.0	30.600685	1
GTATTGG	1130	0.0	26.522123	1
ATTGGAC	1280	0.0	24.570312	3
ACGTTTA	140	0.0	23.785713	26
TATTGGA	1370	0.0	21.875912	2
TTGGACC	2185	0.0	21.336384	4
GGACCCT	2075	0.0	21.130121	6
TGGACCC	2215	0.0	20.295712	5
GCCGTCA	210	0.0	18.500002	13
GACCCTC	2335	0.0	18.222698	7
CGGGATA	210	0.0	17.61905	24
GTATCAA	18335	0.0	17.102535	2
TTTTTAC	2460	0.0	17.071138	1
AGCCGTC	245	0.0	16.612244	12
CGTCTGT	870	0.0	16.373564	34
ACCCTCG	2680	0.0	15.876865	8
GTTATCA	330	0.0	15.696969	1
GCCGGAA	190	2.7284841E-11	15.578948	15
ATCACGT	235	0.0	14.957446	23
CAATGCG	100	1.0942787E-4	14.8	19
>>END_MODULE