FastQCFastQC Report
Wed 25 May 2016
SRR2088208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623255
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT348691.3292264762670805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328061.2505837213690625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT305171.1633257155709225No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG193830.7388911867126909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118390.45130953719710815No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA101110.3854371763324572No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA100720.38395047374349806No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98500.3754877051601922No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT98210.37438220836327385No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA80220.30580328637513315No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT76500.29162243091121526No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT71770.2735913969476852No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG71460.27240965899235875No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA62010.23638571164450273No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA55440.21134049110742187No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54930.2093963415680138No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT50170.1912509458668715No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42680.16269863204301527No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.154045260563689No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC39450.1503856849600973No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36930.14077929900066902No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36440.1389113906196691No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35730.13620482949617937No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34520.13159223941248563No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34520.13159223941248563No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.11279879386487399No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.109482303474119No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC28160.10734755103869048No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27650.10540340149928237No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC26680.10170570531648658No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA26340.10040960562354784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77300.029.7723161
GTATTGG8950.020.8770961
ACGTTTA1500.019.73333426
ATTGGAC10700.018.8457953
TACCCCG1002.8784234E-718.55
TCACGTT1700.018.524
GGACCCT16050.018.2118366
CCGTTTA752.0685393E-417.26666627
TTGGACC17400.017.2241384
TACGCAG650.001580664517.07692312
AGTCGTG650.001580664517.0769237
GTATCAA136200.016.9107932
GACCCTC16700.016.7275457
TATTGGA12200.016.6803282
AGATCGG904.449456E-516.44444517
TTAGTAC1352.222805E-816.4444453
TGGACCC18000.016.0333335
CTAGACG700.00259368215.8571434
GTACGGT700.00259368215.8571436
TTTTTAC17500.015.7514291
Produced by FastQC (version 0.11.5)