##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088208_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2623255
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209596855814628	31.0	31.0	34.0	30.0	34.0
2	31.354739436310997	31.0	31.0	34.0	30.0	34.0
3	31.40549165064014	31.0	31.0	34.0	28.0	34.0
4	35.34816554242725	37.0	35.0	37.0	33.0	37.0
5	35.229902925945055	37.0	35.0	37.0	33.0	37.0
6	35.290593175272704	37.0	35.0	37.0	32.0	37.0
7	35.14182456528245	37.0	35.0	37.0	32.0	37.0
8	35.20519812218027	37.0	35.0	37.0	32.0	37.0
9	36.715844246937486	39.0	35.0	39.0	32.0	39.0
10	36.57776960303135	38.0	35.0	39.0	32.0	39.0
11	36.605947953973214	39.0	35.0	39.0	32.0	39.0
12	36.46454576470835	38.0	35.0	39.0	32.0	39.0
13	36.51198606311624	38.0	35.0	39.0	32.0	39.0
14	37.47549895073106	39.0	36.0	41.0	32.0	41.0
15	37.52659081942091	39.0	36.0	41.0	32.0	41.0
16	37.49607796420859	39.0	36.0	41.0	32.0	41.0
17	37.59040047574483	39.0	36.0	41.0	32.0	41.0
18	37.59957038107237	39.0	36.0	41.0	32.0	41.0
19	37.69396227206276	39.0	37.0	41.0	32.0	41.0
20	37.657953191740795	39.0	37.0	41.0	32.0	41.0
21	37.542601462686626	39.0	36.0	41.0	32.0	41.0
22	37.501889827714045	39.0	36.0	41.0	32.0	41.0
23	37.408113202872	39.0	36.0	41.0	32.0	41.0
24	37.31032209983398	39.0	36.0	41.0	31.0	41.0
25	37.234792271433776	39.0	36.0	41.0	31.0	41.0
26	36.99033567076018	39.0	36.0	40.0	31.0	41.0
27	36.82478943145062	39.0	35.0	40.0	31.0	41.0
28	36.683453571993574	38.0	35.0	40.0	30.0	41.0
29	36.5508454191453	38.0	35.0	40.0	30.0	41.0
30	36.37117550524063	38.0	35.0	40.0	30.0	41.0
31	36.12942432207315	38.0	35.0	40.0	30.0	41.0
32	35.87130378098965	38.0	35.0	40.0	29.0	41.0
33	35.995975991659215	38.0	35.0	40.0	30.0	41.0
34	35.9738588128108	38.0	35.0	40.0	30.0	41.0
35	35.86095023167782	38.0	35.0	40.0	29.0	41.0
36	35.77458767828519	38.0	35.0	40.0	29.0	41.0
37	35.63577578237724	38.0	35.0	40.0	27.0	41.0
38	35.50905992745654	38.0	34.0	40.0	27.0	41.0
39	35.438500641378745	38.0	34.0	40.0	26.0	41.0
40	35.265533087709734	38.0	34.0	40.0	25.0	41.0
41	35.12949332032151	38.0	34.0	40.0	25.0	41.0
42	34.91394965415105	38.0	34.0	40.0	24.0	41.0
43	34.5946310976249	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	1.0
14	2.0
15	12.0
16	25.0
17	61.0
18	169.0
19	334.0
20	777.0
21	1541.0
22	2816.0
23	5037.0
24	8302.0
25	13308.0
26	20485.0
27	30168.0
28	43023.0
29	58992.0
30	76001.0
31	94337.0
32	116032.0
33	144554.0
34	177909.0
35	218113.0
36	276697.0
37	369145.0
38	464678.0
39	500732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04873525448346	22.665581500845324	13.938332339021558	22.34735090564966
2	17.38565255760496	22.82725087724983	37.10443704481646	22.68265952032875
3	19.750615170846906	26.433228946480614	32.31984690775392	21.496308974918566
4	12.693733548587538	15.583159090519223	35.53497467840526	36.188132682487975
5	10.989324331793897	40.75505431229522	34.87461188485298	13.381009471057903
6	31.425309396150965	35.928645899845804	15.678041212158178	16.968003491845053
7	24.399114840150883	35.48804824540504	21.544340904715707	18.56849600972837
8	27.669174365435307	33.705110635451	20.309272259082707	18.316442740030993
9	25.266319896464505	13.642364162081078	23.981465774390976	37.10985016706344
10	16.27542880886532	27.595982853363477	33.777806579993175	22.350781757778027
11	31.192468898372443	21.653861328769032	25.60136166708917	21.552308105769356
12	20.15800980080091	27.235819621043323	32.2262608858079	20.379909692347866
13	31.042578780941998	20.879403641658932	29.168609227848606	18.909408349550464
14	20.743046329845935	22.510202020009494	28.779855561125395	27.966896089019176
15	22.76008241669224	28.817061246428576	27.575512102330883	20.8473442345483
16	21.568852437143928	28.587232274407175	27.435952661864743	22.407962626584148
17	19.022550228628173	27.06949953397592	28.262826145380455	25.645124092015454
18	20.271037318141012	26.559255581329307	32.847664447413614	20.32204265311607
19	19.511141692286873	24.77399261604381	34.164959182389815	21.549906509279502
20	24.249872772566906	23.649588011840255	32.917310745619474	19.183228469973372
21	23.49744878023677	24.7048037647884	31.535630352367573	20.262117102607256
22	21.6591982098576	24.19722825268607	32.59099858763254	21.55257494982379
23	20.547716482004226	26.341815797549227	33.740448412373176	19.370019308073367
24	19.31809907919741	25.394061957377378	32.00005336881089	23.287785594614324
25	20.45851432666668	26.444322035029	33.202033351694745	19.895130286609575
26	22.163990919678035	25.27443957983498	31.748228822588732	20.81334067789826
27	20.50868100889925	26.93722112413776	31.206992839049196	21.34710502791379
28	20.212293505587525	26.153423895122664	31.487445940253618	22.146836659036197
29	19.38770725682406	26.135850308109582	32.46794535796177	22.00849707710459
30	18.66330189020892	26.33514469618851	34.674745688085984	20.32680772551658
31	20.151872387549055	25.773018635245144	33.36160609624302	20.71350288096277
32	21.446066051527588	25.873542602606303	31.200054893634054	21.480336452232056
33	22.375217049047844	25.782777503521388	30.698311830149947	21.14369361728082
34	20.50403029823635	28.776195985521802	30.712873891405906	20.006899824835937
35	19.058879140609662	29.776556224995282	30.895395224635042	20.269169409760014
36	20.184008035818096	25.808394532746533	33.231386197681886	20.776211233753486
37	20.254912313137684	26.062849398933768	32.29034157944996	21.39189670847859
38	20.59620585875182	25.547344806356985	32.971746932722894	20.884702402168298
39	21.439471191325282	25.038968762091372	32.88852970832039	20.63303033826296
40	19.53511953660624	26.07504798427907	31.87238754905642	22.517444930058268
41	20.629218280342553	25.532287177571376	31.69051426567375	22.14798027641232
42	18.11360313808608	27.344768236408584	32.20430343218635	22.33732519331899
43	18.86892429443573	27.06660236995641	31.72661445418002	22.337858881427845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	264.0
2	368.0
3	1112.5
4	1857.0
5	1857.0
6	3332.0
7	4807.0
8	5312.0
9	5817.0
10	8779.0
11	11741.0
12	11741.0
13	21501.5
14	31262.0
15	50381.5
16	69501.0
17	68642.0
18	67783.0
19	67783.0
20	71799.5
21	75816.0
22	60910.0
23	46004.0
24	50250.0
25	54496.0
26	54496.0
27	59436.5
28	64377.0
29	67842.0
30	71307.0
31	76417.0
32	81527.0
33	81527.0
34	88567.5
35	95608.0
36	103942.5
37	112277.0
38	116950.0
39	121623.0
40	121623.0
41	128738.5
42	135854.0
43	155759.5
44	175665.0
45	223871.5
46	272078.0
47	272078.0
48	273465.0
49	274852.0
50	248783.5
51	222715.0
52	206114.5
53	189514.0
54	189514.0
55	163215.0
56	136916.0
57	110735.0
58	84554.0
59	73834.0
60	63114.0
61	63114.0
62	55656.0
63	48198.0
64	41605.0
65	35012.0
66	29840.5
67	24669.0
68	24669.0
69	20711.0
70	16753.0
71	14068.0
72	11383.0
73	9034.5
74	6686.0
75	6686.0
76	5285.0
77	3884.0
78	3117.0
79	2350.0
80	1812.0
81	1274.0
82	1274.0
83	940.0
84	606.0
85	489.5
86	373.0
87	326.0
88	279.0
89	279.0
90	216.5
91	154.0
92	92.0
93	30.0
94	19.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2623255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.17988548234563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78463066022435	30.384426409452065
2	13.755596000153503	11.329077360554656
3	5.150014889843364	6.362310701883736
4	2.4140003467509925	3.97633031334194
5	1.2709613572005052	2.616902157100169
6	0.7560974115788132	1.8681602893388085
7	0.49099137616340527	1.4153278050259819
8	0.3347346816399878	1.1027468685523296
9	0.2511944874186349	0.9309744203126308
>10	1.519679808737923	12.050603808472513
>50	0.16236033689429638	4.668570856178117
>100	0.09412192037732459	7.162725707488196
>500	0.007157664716026489	2.016370359350585
>1k	0.0068787946621552375	5.635884347549762
>5k	9.295668462371942E-4	2.7530967748429425
>10k+	6.50696792366036E-4	5.726491820555605
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34869	1.3292264762670805	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32806	1.2505837213690625	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30517	1.1633257155709225	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19383	0.7388911867126909	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11839	0.45130953719710815	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10111	0.3854371763324572	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10072	0.38395047374349806	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9850	0.3754877051601922	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9821	0.37438220836327385	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8022	0.30580328637513315	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7650	0.29162243091121526	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7177	0.2735913969476852	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7146	0.27240965899235875	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6201	0.23638571164450273	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5544	0.21134049110742187	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5493	0.2093963415680138	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5017	0.1912509458668715	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4268	0.16269863204301527	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4041	0.154045260563689	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3945	0.1503856849600973	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3693	0.14077929900066902	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3644	0.1389113906196691	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3573	0.13620482949617937	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3452	0.13159223941248563	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3452	0.13159223941248563	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.11279879386487399	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2872	0.109482303474119	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2816	0.10734755103869048	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2765	0.10540340149928237	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2668	0.10170570531648658	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2634	0.10040960562354784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.812057920408043E-5	0.0
8	0.0	0.0	0.0	3.812057920408043E-5	0.0
9	0.0	0.0	0.0	7.624115840816086E-5	0.0
10	0.0	0.0	0.0	3.0496463363264344E-4	0.0
11	0.0	0.0	0.0	6.480498464693673E-4	0.0
12	0.0	0.0	0.0	0.0011054967969183324	0.0
13	0.0	0.0	0.0	0.001334220272142815	0.0
14	7.624115840816086E-5	0.0	0.0	0.0016391849057754585	0.0
15	7.624115840816086E-5	0.0	0.0	0.002210993593836665	0.0
16	7.624115840816086E-5	0.0	0.0	0.004345746029265169	0.0
17	7.624115840816086E-5	0.0	0.0	0.006404257306285512	0.0
18	7.624115840816086E-5	0.0	0.0	0.007776598157632407	0.0
19	7.624115840816086E-5	0.0	0.0	0.0089583361129589	0.0
20	7.624115840816086E-5	0.0	0.0	0.011359932602815967	0.0
21	7.624115840816086E-5	0.0	0.0	0.015705678632081135	0.0
22	7.624115840816086E-5	0.0	0.0	0.028094866873407273	0.0
23	7.624115840816086E-5	0.0	0.0	0.046202141995345475	0.0
24	7.624115840816086E-5	0.0	0.0	0.06957005704744677	0.0
25	7.624115840816086E-5	0.0	0.0	0.078680875477222	0.0
26	7.624115840816086E-5	0.0	0.0	0.08969772286720125	0.0
27	7.624115840816086E-5	0.0	0.0	0.17249562089846393	0.0
28	7.624115840816086E-5	0.0	0.0	0.25731390962754286	0.0
29	7.624115840816086E-5	0.0	0.0	0.3534158898010296	0.0
30	7.624115840816086E-5	0.0	0.0	0.550308681390105	0.0
31	7.624115840816086E-5	0.0	0.0	0.826149192510831	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7730	0.0	29.772316	1
GTATTGG	895	0.0	20.877096	1
ACGTTTA	150	0.0	19.733334	26
ATTGGAC	1070	0.0	18.845795	3
TACCCCG	100	2.8784234E-7	18.5	5
TCACGTT	170	0.0	18.5	24
GGACCCT	1605	0.0	18.211836	6
CCGTTTA	75	2.0685393E-4	17.266666	27
TTGGACC	1740	0.0	17.224138	4
TACGCAG	65	0.0015806645	17.076923	12
AGTCGTG	65	0.0015806645	17.076923	7
GTATCAA	13620	0.0	16.910793	2
GACCCTC	1670	0.0	16.727545	7
TATTGGA	1220	0.0	16.680328	2
AGATCGG	90	4.449456E-5	16.444445	17
TTAGTAC	135	2.222805E-8	16.444445	3
TGGACCC	1800	0.0	16.033333	5
CTAGACG	70	0.002593682	15.857143	4
GTACGGT	70	0.002593682	15.857143	6
TTTTTAC	1750	0.0	15.751429	1
>>END_MODULE