FastQCFastQC Report
Wed 25 May 2016
SRR2088207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3252147
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT451531.388405874642198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT431161.3257703295699732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT395481.2160581917115063No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG259930.7992566141690397No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160150.49244391474309124No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA140390.43168405364210166No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT133820.41148201480437385No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA131530.40444051268285225No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT127860.39315565993788104No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA103780.3191122664504403No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT99740.30668970375570354No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT99320.3053982492181319No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG98240.30207736612151914No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA85440.2627187516431453No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA77670.23882684269806992No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA76070.2339070158882732No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT67130.20641748358853398No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA54890.168780808493589No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC52610.16177005528962865No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.1613088215262102No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48840.15017771336904512No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC47650.1465185921792588No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47320.14550387789973823No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT45760.14070704676018642No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG44650.13729391691088996No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.12035126333465246No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.11890606420927466No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG36040.1108190988906713No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC35920.11045011187993654No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC35260.1084206833208954No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA34660.10657574826722163No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA33700.10362385218134358No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC32760.10073345393058801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98050.029.905661
GTATTGG13300.023.0902271
ATTGGAC12550.022.8486063
TTGGACC20850.020.0527574
GGACCCT20350.019.4545466
TGGACCC21300.018.8474185
TAGTGCG902.1546803E-618.57
CGTCTGT10150.018.226634
TATTGGA16600.017.9427722
CGACGAG1851.8189894E-1217.00000224
GACCCTC23400.016.9188027
CAATGCG1655.4569682E-1116.81818219
GTATCAA175750.016.715792
TCTATAC2550.016.6862753
TGCGACG2000.016.6522
TCACGTT1801.0913936E-1116.44444524
TGAACCG1151.2438286E-616.0869565
CGCGCTT700.002593861415.85714217
ATCACGT1954.1836756E-1115.17948723
TGCGTTA1853.0559022E-1015.00000137
Produced by FastQC (version 0.11.5)