##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088207_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3252147
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178274536790617	31.0	31.0	34.0	30.0	34.0
2	31.335404580420256	31.0	31.0	34.0	30.0	34.0
3	31.379397979242636	31.0	31.0	34.0	28.0	34.0
4	35.32051810696134	37.0	35.0	37.0	33.0	37.0
5	35.21030168685487	37.0	35.0	37.0	32.0	37.0
6	35.26676377174832	37.0	35.0	37.0	32.0	37.0
7	35.1040807810963	37.0	35.0	37.0	32.0	37.0
8	35.17279108232192	37.0	35.0	37.0	32.0	37.0
9	36.66466214473085	39.0	35.0	39.0	32.0	39.0
10	36.538320069787744	38.0	35.0	39.0	32.0	39.0
11	36.56622963230137	39.0	35.0	39.0	32.0	39.0
12	36.42038136652494	38.0	35.0	39.0	32.0	39.0
13	36.45400961272661	38.0	35.0	39.0	32.0	39.0
14	37.39699619974128	39.0	36.0	41.0	32.0	41.0
15	37.446846959869895	39.0	36.0	41.0	32.0	41.0
16	37.418884201728886	39.0	36.0	41.0	32.0	41.0
17	37.53116264424702	39.0	36.0	41.0	32.0	41.0
18	37.542473326082735	39.0	36.0	41.0	32.0	41.0
19	37.64318464079268	39.0	37.0	41.0	32.0	41.0
20	37.61482798901772	39.0	36.0	41.0	32.0	41.0
21	37.48792566879664	39.0	36.0	41.0	32.0	41.0
22	37.44646382835708	39.0	36.0	41.0	32.0	41.0
23	37.32822747557229	39.0	36.0	41.0	32.0	41.0
24	37.229372473015516	39.0	36.0	41.0	31.0	41.0
25	37.15963546543253	39.0	36.0	41.0	31.0	41.0
26	36.905176180535506	39.0	35.0	40.0	31.0	41.0
27	36.740534483834836	39.0	35.0	40.0	30.0	41.0
28	36.59915311331253	38.0	35.0	40.0	30.0	41.0
29	36.45824681356655	38.0	35.0	40.0	30.0	41.0
30	36.26822803520259	38.0	35.0	40.0	30.0	41.0
31	35.999414540609635	38.0	35.0	40.0	30.0	41.0
32	35.721620517153745	38.0	34.0	40.0	29.0	41.0
33	35.84864460308836	38.0	35.0	40.0	30.0	41.0
34	35.82166427286344	38.0	35.0	40.0	29.0	41.0
35	35.71828671951176	38.0	35.0	40.0	29.0	41.0
36	35.621728046118456	38.0	34.0	40.0	28.0	41.0
37	35.47108356418083	38.0	34.0	40.0	27.0	41.0
38	35.34290608634849	38.0	34.0	40.0	26.0	41.0
39	35.26324394315509	38.0	34.0	40.0	25.0	41.0
40	35.07472755690318	38.0	34.0	40.0	25.0	41.0
41	34.92240387657753	38.0	34.0	40.0	24.0	41.0
42	34.67832235135742	38.0	33.0	40.0	23.0	41.0
43	34.363547527218174	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	7.0
15	13.0
16	31.0
17	90.0
18	191.0
19	491.0
20	983.0
21	2098.0
22	3784.0
23	6699.0
24	10873.0
25	17873.0
26	26981.0
27	39391.0
28	55844.0
29	76036.0
30	97677.0
31	120924.0
32	148288.0
33	182631.0
34	223887.0
35	273047.0
36	345888.0
37	460424.0
38	572389.0
39	585601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90005771571826	22.909450280076516	14.071996130556213	22.118495873649007
2	17.283136340392975	22.924117513753224	37.214676950334656	22.578069195519145
3	19.88025141544955	26.427372440421664	32.474085581002335	21.218290563126452
4	12.568650802070138	15.464983593915035	35.54344253196427	36.42292307205056
5	10.9050728641725	40.88877286297329	34.7505509437304	13.455603329123806
6	31.33287025463486	35.85173117943315	15.543916065294711	17.271482500637273
7	24.273687505515586	35.692144297290376	21.349742185700705	18.68442601149333
8	27.86343913728377	33.46623015503297	20.26611958192542	18.404211125757847
9	25.110273305603958	13.63652996005408	24.347423409827414	36.90577332451455
10	16.290284541258437	27.70791111225907	33.74257067715574	22.259233669326754
11	30.845991893970353	21.652557525843697	25.897722335429485	21.603728244756464
12	20.24668626602672	27.223953898762883	32.23359829675596	20.295761538454443
13	31.267959289663104	20.7783965484955	29.385233816306584	18.56841034553481
14	20.768649141628593	22.582773779906013	28.91154674127584	27.737030337189555
15	22.678771900532173	28.882120027169744	27.725007510423115	20.71410056187497
16	21.428213423316965	28.63591344425698	27.626026744793514	22.30984638763254
17	18.98825606591584	26.94659866236059	28.333251848701796	25.73189342302178
18	20.216060344135737	26.39619303801458	33.206371052723014	20.181375565126668
19	19.21383012514502	24.61687617441647	34.757346454511435	21.411947245927077
20	24.37528192913789	23.311922862035452	33.21574947257919	19.09704573624747
21	23.605298284487137	24.505749586350188	31.834477346811198	20.054474782351477
22	21.606065162491117	24.057799355318192	32.8644738383597	21.471661643830984
23	20.52853084439295	26.212775744761846	34.1827721809623	19.075921229882905
24	19.036654862157214	25.28960099282105	32.29792503229405	23.375819112727683
25	20.377492161332192	26.33094383494965	33.6134867212337	19.678077282484463
26	22.01954585693697	25.138777552183218	31.99477145405789	20.84690513682192
27	20.476165437786175	26.88740699605522	31.542085889721466	21.094341676437132
28	20.200747383190244	26.129384680335793	31.683930646431417	21.98593729004255
29	19.087698065308857	26.18611643323626	32.799747366893314	21.926438134561568
30	18.505682553709903	26.282114553862417	35.04813896788798	20.164063924539697
31	20.074892063612133	25.76620921502011	33.6046002840585	20.55429843730926
32	21.43131906399065	25.92410490669702	31.25240033737712	21.392175691935204
33	22.43318029597063	25.874506902670756	30.689203163325647	21.003109638032967
34	20.377153923239018	29.089705969625605	30.739293150032886	19.79384695710249
35	18.96494838640443	30.195375547292297	30.9162224216802	19.923453644623077
36	19.969638518800043	25.9979638066791	33.49876250981275	20.533635164708112
37	20.10487225823433	26.398314713326304	32.37605803181714	21.12075499662223
38	20.443879074346885	25.79028561747055	33.08555855562494	20.68027675255762
39	21.519506959556256	25.20627142623012	32.98507109303485	20.28915052117878
40	19.60692428724778	26.25010493068118	31.805112130540223	22.33785865153082
41	20.833929093611083	25.660648181032407	31.642788594734494	21.862634130622016
42	18.010963219067282	27.705205207513682	32.10030173912803	22.183529834291008
43	18.786143430785877	27.460167083468246	31.55755259525477	22.196136890491115
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	257.0
1	360.5
2	464.0
3	1463.5
4	2463.0
5	2463.0
6	4419.5
7	6376.0
8	6917.0
9	7458.0
10	11378.0
11	15298.0
12	15298.0
13	28034.0
14	40770.0
15	65787.0
16	90804.0
17	89578.0
18	88352.0
19	88352.0
20	93291.5
21	98231.0
22	79394.5
23	60558.0
24	65681.5
25	70805.0
26	70805.0
27	76204.5
28	81604.0
29	84790.5
30	87977.0
31	93688.5
32	99400.0
33	99400.0
34	107272.0
35	115144.0
36	125001.0
37	134858.0
38	140095.5
39	145333.0
40	145333.0
41	153224.0
42	161115.0
43	186214.5
44	211314.0
45	275565.0
46	339816.0
47	339816.0
48	343791.0
49	347766.0
50	313878.5
51	279991.0
52	259074.0
53	238157.0
54	238157.0
55	203183.0
56	168209.0
57	135115.5
58	102022.0
59	88294.0
60	74566.0
61	74566.0
62	65230.5
63	55895.0
64	48987.5
65	42080.0
66	35709.0
67	29338.0
68	29338.0
69	25027.0
70	20716.0
71	17688.5
72	14661.0
73	11778.0
74	8895.0
75	8895.0
76	6941.5
77	4988.0
78	3897.0
79	2806.0
80	2233.5
81	1661.0
82	1661.0
83	1250.5
84	840.0
85	671.0
86	502.0
87	434.0
88	366.0
89	366.0
90	290.0
91	214.0
92	133.5
93	53.0
94	36.0
95	19.0
96	19.0
97	11.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3252147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.32869745342749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.93004582441952	25.767961751130848
2	14.092718050356082	10.239401806956852
3	5.835044837939991	6.359387356341173
4	2.8361179808622152	4.121298882758763
5	1.6280613392060352	2.9572673913819023
6	1.0209775070368738	2.2254469775938337
7	0.6483326605042277	1.6487156750843661
8	0.4623365118141495	1.3436866607495392
9	0.33836291743172836	1.1063055651152696
>10	1.8783759834245881	12.78219271819174
>50	0.18861600878785964	4.764092777959435
>100	0.12029674614417092	8.038580107473738
>500	0.010910884383977642	2.6672847678234293
>1k	0.00809792200371727	6.381069964553165
>5k	8.524128424965547E-4	2.3645509040249735
>10k+	8.524128424965547E-4	7.232756692860967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45153	1.388405874642198	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43116	1.3257703295699732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39548	1.2160581917115063	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	25993	0.7992566141690397	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16015	0.49244391474309124	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14039	0.43168405364210166	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	13382	0.41148201480437385	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13153	0.40444051268285225	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	12786	0.39315565993788104	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	10378	0.3191122664504403	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9974	0.30668970375570354	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9932	0.3053982492181319	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9824	0.30207736612151914	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8544	0.2627187516431453	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7767	0.23882684269806992	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7607	0.2339070158882732	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6713	0.20641748358853398	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5489	0.168780808493589	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5261	0.16177005528962865	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5246	0.1613088215262102	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4884	0.15017771336904512	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4765	0.1465185921792588	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4732	0.14550387789973823	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4576	0.14070704676018642	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4465	0.13729391691088996	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3914	0.12035126333465246	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3867	0.11890606420927466	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3604	0.1108190988906713	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3592	0.11045011187993654	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3526	0.1084206833208954	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3466	0.10657574826722163	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3370	0.10362385218134358	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3276	0.10073345393058801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	3.074891756122955E-5	0.0
7	0.0	0.0	0.0	3.074891756122955E-5	0.0
8	0.0	0.0	0.0	3.074891756122955E-5	0.0
9	0.0	0.0	0.0	6.14978351224591E-5	0.0
10	0.0	0.0	0.0	1.229956702449182E-4	0.0
11	0.0	0.0	0.0	2.459913404898364E-4	0.0
12	0.0	0.0	0.0	7.072251039082797E-4	0.0
13	0.0	0.0	0.0	7.994718565919683E-4	0.0
14	0.0	0.0	0.0	9.532164443981161E-4	0.0
15	0.0	0.0	0.0	0.0014759480429390184	0.0
16	0.0	0.0	0.0	0.0026444069102657414	0.0
17	0.0	0.0	0.0	0.004274099541010908	0.0
18	0.0	0.0	0.0	0.004858328974674269	0.0
19	0.0	0.0	0.0	0.005596302996143779	0.0
20	3.074891756122955E-5	0.0	0.0	0.007287493462011404	0.0
21	3.074891756122955E-5	0.0	0.0	0.010208640630328211	0.0
22	3.074891756122955E-5	0.0	0.0	0.016942653576237483	0.0
23	3.074891756122955E-5	0.0	0.0	0.02927296951829053	0.0
24	3.074891756122955E-5	0.0	0.0	0.044985666392078835	0.0
25	3.074891756122955E-5	0.0	0.0	0.05153518583262073	0.0
26	3.074891756122955E-5	0.0	0.0	0.05952990439854041	0.0
27	3.074891756122955E-5	0.0	0.0	0.12859197324106197	0.0
28	3.074891756122955E-5	0.0	0.0	0.2030966004919212	0.0
29	3.074891756122955E-5	0.0	0.0	0.28943956100385376	0.0
30	3.074891756122955E-5	0.0	0.0	0.4670760577550769	0.0
31	3.074891756122955E-5	0.0	0.0	0.71623453675372	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9805	0.0	29.90566	1
GTATTGG	1330	0.0	23.090227	1
ATTGGAC	1255	0.0	22.848606	3
TTGGACC	2085	0.0	20.052757	4
GGACCCT	2035	0.0	19.454546	6
TGGACCC	2130	0.0	18.847418	5
TAGTGCG	90	2.1546803E-6	18.5	7
CGTCTGT	1015	0.0	18.2266	34
TATTGGA	1660	0.0	17.942772	2
CGACGAG	185	1.8189894E-12	17.000002	24
GACCCTC	2340	0.0	16.918802	7
CAATGCG	165	5.4569682E-11	16.818182	19
GTATCAA	17575	0.0	16.71579	2
TCTATAC	255	0.0	16.686275	3
TGCGACG	200	0.0	16.65	22
TCACGTT	180	1.0913936E-11	16.444445	24
TGAACCG	115	1.2438286E-6	16.086956	5
CGCGCTT	70	0.0025938614	15.857142	17
ATCACGT	195	4.1836756E-11	15.179487	23
TGCGTTA	185	3.0559022E-10	15.000001	37
>>END_MODULE