FastQCFastQC Report
Wed 25 May 2016
SRR2088206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459715
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT268021.836111843750321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT259641.7787033770290777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT222341.5231740442483634No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG135850.930661122205362No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA87570.5999116265846415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85320.5844976587895582No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT82600.5658638843883909No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT72370.4957817108134122No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71880.4924248911602608No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60560.41487550652010835No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58610.40151673443103625No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG56240.38528068835354845No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT52870.36219398992269036No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48800.334311834844473No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48180.33006443038538347No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46570.3190348800964572No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT42740.29279688158304873No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39090.267792000493247No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.22017996663732303No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31790.21778223831364343No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC31250.21408288604282344No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30440.20853385763659346No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27650.18942053757069016No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT27550.18873547233535312No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26530.18174780693491538No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.16832052832230948No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23140.1585240954569899No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC22010.1507828582976814No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC21020.14400071246784477No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA20930.1433841537560414No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC20750.14215103633243475No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC20610.1411919450029629No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC20540.140712399338227No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA19930.13653350140267106No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA19810.13571142312026663No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG19790.13557441007319923No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19390.1328341491318511No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19270.13201207084944663No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA18920.12961434252576703No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC18800.12879226424336257No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG18750.12844973162569406No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA18730.12831271857862667No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG18580.1272851207256211No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA18180.12454485978427296No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT18090.12392830107246962No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA17570.12036596184871705No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA16990.11639258348376223No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16960.11618706391316114No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG16570.1135153094953467No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG16450.11269323121294225No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT16180.11084355507753225No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA16110.11036400941279634No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC15960.10933641155979079No Hit
GTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAAAA15630.10707569628317856No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT15360.10522602014776856No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA15340.10508900710070115No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC14880.1019377070181508No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC14800.10138965482988117No Hit
TCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATC14760.10111562873574637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55700.031.4201091
GTATTGG3550.026.0563371
GTCCTAC851.9772415E-923.94117530
TGCGACG851.9772415E-923.94117522
CAATGCG851.9772415E-923.94117519
TCACGTT701.9236722E-723.78571324
ATTGGAC3850.023.5454543
ATCGTCA952.8194336E-1023.36842210
TATTGGA4150.022.7349382
CGTCTGT5650.022.26548834
CGCGTCA502.7025875E-422.19999932
CGACGAG957.1486284E-921.42105324
CACGGCT1059.840733E-1021.1428574
ATCGCAG705.103482E-621.14285711
GTAGACC806.962764E-720.81253
CGTGTCT1700.020.6764735
ACCGTAC450.003826295520.5555558
TCCACGG1351.8189894E-1220.5555532
CTAGTAC1001.290573E-820.3499983
ATTCGCG656.903762E-519.92307729
Produced by FastQC (version 0.11.5)