##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088206_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1459715
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10392234100492	31.0	31.0	33.0	30.0	34.0
2	31.263615842818634	31.0	31.0	34.0	28.0	34.0
3	31.297177188697795	31.0	31.0	34.0	28.0	34.0
4	35.26220803376002	37.0	35.0	37.0	33.0	37.0
5	35.15681006223818	37.0	35.0	37.0	32.0	37.0
6	35.22470345238625	37.0	35.0	37.0	32.0	37.0
7	35.04104636864045	37.0	35.0	37.0	32.0	37.0
8	35.124261242776846	37.0	35.0	37.0	32.0	37.0
9	36.563478487238946	39.0	35.0	39.0	32.0	39.0
10	36.45337617274605	38.0	35.0	39.0	32.0	39.0
11	36.49586528877212	38.0	35.0	39.0	32.0	39.0
12	36.33551275420202	38.0	35.0	39.0	32.0	39.0
13	36.37020582784996	38.0	35.0	39.0	32.0	39.0
14	37.24170403126638	39.0	36.0	41.0	32.0	41.0
15	37.290873218402226	39.0	36.0	41.0	32.0	41.0
16	37.25692070027368	39.0	36.0	41.0	32.0	41.0
17	37.4139951976927	39.0	36.0	41.0	32.0	41.0
18	37.45397149443556	39.0	36.0	41.0	32.0	41.0
19	37.572175390401554	39.0	36.0	41.0	32.0	41.0
20	37.557596517128346	39.0	36.0	41.0	32.0	41.0
21	37.433720966078994	39.0	36.0	41.0	32.0	41.0
22	37.39597798200334	39.0	36.0	41.0	32.0	41.0
23	37.26790092586567	39.0	36.0	41.0	31.0	41.0
24	37.15850354349993	39.0	36.0	41.0	31.0	41.0
25	37.08868374991008	39.0	36.0	40.0	31.0	41.0
26	36.80893667599497	39.0	35.0	40.0	31.0	41.0
27	36.62666342402455	38.0	35.0	40.0	30.0	41.0
28	36.46881959834625	38.0	35.0	40.0	30.0	41.0
29	36.29258314122962	38.0	35.0	40.0	30.0	41.0
30	36.07443644821078	38.0	35.0	40.0	30.0	41.0
31	35.74419801125562	38.0	34.0	40.0	29.0	41.0
32	35.46895387113238	38.0	34.0	40.0	28.0	41.0
33	35.538113261835356	38.0	34.0	40.0	29.0	41.0
34	35.48159949031147	38.0	34.0	40.0	28.0	41.0
35	35.34499953758097	38.0	34.0	40.0	27.0	41.0
36	35.228063012300346	38.0	34.0	40.0	26.0	41.0
37	35.05038312273286	38.0	34.0	40.0	25.0	41.0
38	34.891504848549204	38.0	34.0	40.0	25.0	41.0
39	34.75177414769321	38.0	33.0	40.0	24.0	41.0
40	34.4945020089538	38.0	33.0	40.0	23.0	41.0
41	34.29331410583573	38.0	33.0	40.0	21.0	41.0
42	33.97010649339083	38.0	33.0	40.0	20.0	41.0
43	33.625883134721505	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	6.0
16	14.0
17	41.0
18	105.0
19	229.0
20	521.0
21	1026.0
22	1875.0
23	3334.0
24	5514.0
25	9051.0
26	13702.0
27	19616.0
28	27661.0
29	37374.0
30	47217.0
31	58005.0
32	70787.0
33	85964.0
34	104585.0
35	125820.0
36	158641.0
37	211536.0
38	244294.0
39	232796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28642577489441	23.622145418797505	15.039853670065732	21.05157513624235
2	17.236035801509196	23.28296962078214	37.126836402996474	22.35415817471219
3	20.206752688024718	25.68391775106785	33.79878948972916	20.31054007117828
4	12.54888796785674	15.29634209417592	34.44631315016973	37.708456787797616
5	10.354212979931015	41.57421140428097	34.35581603258171	13.71575958320631
6	31.33282866860997	35.04088126791874	14.952370839513193	18.6739192239581
7	23.348324844233293	37.267822828428834	20.222303668866868	19.161548658471002
8	28.71293368911055	32.68364029964753	19.96081426853872	18.6426117427032
9	24.874855708134806	13.526818591300357	25.516282287980875	36.082043412583964
10	16.756147604155604	28.391980626355146	32.972669322436225	21.87920244705302
11	30.09066838389686	21.276961598668233	26.332880048502616	22.299489968932292
12	20.731923697434087	28.100142836101565	31.467581000400763	19.70035246606358
13	32.067903666126604	20.71808537968028	30.27803372576154	16.93597722843158
14	20.755969487194417	23.090671809223036	29.493976563918302	26.65938213966425
15	22.80088921467547	29.068277026679866	28.386637117519513	19.74419664112515
16	21.38102300791593	28.447881949558646	28.102883097042913	22.068211945482506
17	18.259043717437994	27.165508335531253	28.331146833457215	26.244301113573542
18	19.61841866391727	26.218542660724868	34.7881607026029	19.374877972754955
19	18.277197946174425	24.14779597387161	36.69181997855746	20.883186101396507
20	24.543695173372885	22.66976772863196	34.86420294372532	17.92233415426984
21	23.722987021439117	23.978653367266897	33.13290608098156	19.165453530312423
22	20.89818903005039	23.743675991546294	34.25689261259903	21.101242365804286
23	20.04829709909126	26.602864257748944	35.621542561390406	17.727296081769385
24	17.894314986144554	25.22739027823925	33.33260259708231	23.54569213853389
25	19.48784523006203	26.517368116378883	35.30894729450612	18.685839359052967
26	21.565168543174522	25.072428522006007	33.15558174027122	20.20682119454825
27	19.58142514120907	27.189965164432785	32.5425168611681	20.686092833190038
28	19.385770509996814	26.506818111754693	32.56656265092843	21.540848727320057
29	18.114357939734813	26.405839496066015	33.89264342697033	21.58715913722884
30	17.201782539742347	26.43721548384445	37.23007573396176	19.130926242451437
31	19.231151286381245	25.815929821917294	35.19180113926348	19.761117752437976
32	21.01053972864566	26.12167443644821	32.229921594283816	20.637864240622314
33	22.278115933589778	26.207239084341804	31.22575297232679	20.288892009741627
34	19.61711703997013	30.41552631849368	31.2767903323594	18.69056630917679
35	17.542945026940192	31.8194990117934	31.480186200730962	19.15736976053545
36	19.183950291666523	26.34452615750335	34.898182179398034	19.573341371432097
37	19.09064440661362	27.040894969223444	33.44789907618953	20.420561547973406
38	19.715218381670393	26.369531038593152	34.27833515446508	19.63691542527137
39	21.423291532936222	25.45339329937693	33.736928098978225	19.38638706870862
40	18.677892602323055	26.991570272279176	32.40988823160686	21.92064889379091
41	20.226825099420093	26.10591793603546	32.345355086438104	21.321901878106342
42	16.61735338747632	28.62277910414019	33.0114440147563	21.748423493627183
43	17.53184697012773	28.489739435437738	32.367551200063026	21.610862394371505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	181.0
2	211.0
3	674.5
4	1138.0
5	1138.0
6	2155.5
7	3173.0
8	3450.5
9	3728.0
10	5902.0
11	8076.0
12	8076.0
13	14968.0
14	21860.0
15	37000.0
16	52140.0
17	50362.0
18	48584.0
19	48584.0
20	51726.5
21	54869.0
22	42573.5
23	30278.0
24	31499.5
25	32721.0
26	32721.0
27	34412.5
28	36104.0
29	35689.5
30	35275.0
31	37217.5
32	39160.0
33	39160.0
34	41813.0
35	44466.0
36	48683.0
37	52900.0
38	54801.5
39	56703.0
40	56703.0
41	60435.0
42	64167.0
43	78065.0
44	91963.0
45	128835.5
46	165708.0
47	165708.0
48	170128.5
49	174549.0
50	155529.5
51	136510.0
52	124491.0
53	112472.0
54	112472.0
55	94511.0
56	76550.0
57	57364.0
58	38178.0
59	31270.0
60	24362.0
61	24362.0
62	21077.0
63	17792.0
64	15212.5
65	12633.0
66	10644.0
67	8655.0
68	8655.0
69	7257.5
70	5860.0
71	4791.0
72	3722.0
73	2980.0
74	2238.0
75	2238.0
76	1747.0
77	1256.0
78	1007.5
79	759.0
80	592.0
81	425.0
82	425.0
83	304.0
84	183.0
85	140.0
86	97.0
87	78.0
88	59.0
89	59.0
90	45.0
91	31.0
92	19.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1459715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.556116951791054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91418610921751	25.196311426347783
2	13.337472191354538	9.217824977714168
3	5.2144047218298795	5.405687382045744
4	2.552309320051834	3.5279159744342996
5	1.4699375702144732	2.5397667294081456
6	0.8865509342538769	1.8381454660677958
7	0.6044160549183875	1.4620390316909972
8	0.4271838549376805	1.1809452200914745
9	0.3182858830801759	0.9898851779840387
>10	1.9164176757474858	12.559296393396263
>50	0.20226152446749515	4.851495907336585
>100	0.1287432045625278	8.770906416949035
>500	0.011212471225863777	2.776977351338137
>1k	0.01401558903232972	9.208458557577833
>5k	0.0018020043041566784	4.345219544018778
>10k+	8.008908018474126E-4	6.129124443598984
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26802	1.836111843750321	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25964	1.7787033770290777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22234	1.5231740442483634	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	13585	0.930661122205362	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8757	0.5999116265846415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8532	0.5844976587895582	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8260	0.5658638843883909	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7237	0.4957817108134122	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7188	0.4924248911602608	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6056	0.41487550652010835	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5861	0.40151673443103625	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5624	0.38528068835354845	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5287	0.36219398992269036	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4880	0.334311834844473	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4818	0.33006443038538347	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4657	0.3190348800964572	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4274	0.29279688158304873	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3909	0.267792000493247	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3214	0.22017996663732303	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3179	0.21778223831364343	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3125	0.21408288604282344	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3044	0.20853385763659346	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2765	0.18942053757069016	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2755	0.18873547233535312	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2653	0.18174780693491538	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2457	0.16832052832230948	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2314	0.1585240954569899	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2201	0.1507828582976814	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2102	0.14400071246784477	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2093	0.1433841537560414	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2075	0.14215103633243475	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2061	0.1411919450029629	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2054	0.140712399338227	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1993	0.13653350140267106	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1981	0.13571142312026663	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1979	0.13557441007319923	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1939	0.1328341491318511	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1927	0.13201207084944663	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1892	0.12961434252576703	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1880	0.12879226424336257	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	1875	0.12844973162569406	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1873	0.12831271857862667	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1858	0.1272851207256211	No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA	1818	0.12454485978427296	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	1809	0.12392830107246962	No Hit
CTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAAA	1757	0.12036596184871705	No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	1699	0.11639258348376223	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1696	0.11618706391316114	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG	1657	0.1135153094953467	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	1645	0.11269323121294225	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	1618	0.11084355507753225	No Hit
ACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTAA	1611	0.11036400941279634	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1596	0.10933641155979079	No Hit
GTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAAAA	1563	0.10707569628317856	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	1536	0.10522602014776856	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	1534	0.10508900710070115	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC	1488	0.1019377070181508	No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC	1480	0.10138965482988117	No Hit
TCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATC	1476	0.10111562873574637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.85065235337035E-5	0.0	0.0	0.0	0.0
3	6.85065235337035E-5	0.0	0.0	0.0	0.0
4	6.85065235337035E-5	0.0	0.0	0.0	0.0
5	6.85065235337035E-5	0.0	0.0	0.0	0.0
6	6.85065235337035E-5	0.0	0.0	0.0	0.0
7	6.85065235337035E-5	0.0	0.0	0.0	0.0
8	6.85065235337035E-5	0.0	0.0	0.0	0.0
9	6.85065235337035E-5	0.0	0.0	0.0	0.0
10	6.85065235337035E-5	0.0	0.0	1.37013047067407E-4	0.0
11	6.85065235337035E-5	0.0	0.0	2.0551957060111048E-4	0.0
12	6.85065235337035E-5	0.0	0.0	2.0551957060111048E-4	0.0
13	6.85065235337035E-5	0.0	0.0	4.7954566473592446E-4	0.0
14	1.37013047067407E-4	0.0	0.0	6.165587118033315E-4	0.0
15	1.37013047067407E-4	0.0	0.0	0.0011646109000729595	0.0
16	1.37013047067407E-4	0.0	0.0	0.0020551957060111047	0.0
17	1.37013047067407E-4	0.0	0.0	0.0033568196531514713	0.0
18	1.37013047067407E-4	0.0	0.0	0.0041103914120222095	0.0
19	1.37013047067407E-4	0.0	0.0	0.004452924029690727	0.0
20	1.37013047067407E-4	0.0	0.0	0.005823054500364798	0.0
21	1.37013047067407E-4	0.0	0.0	0.0076042241122410885	0.0
22	1.37013047067407E-4	0.0	0.0	0.013016239471403665	0.0
23	1.37013047067407E-4	0.0	0.0	0.019798385301240312	0.0
24	1.37013047067407E-4	0.0	0.0	0.030279883401896947	0.0
25	1.37013047067407E-4	0.0	0.0	0.03713053575526729	0.0
26	1.37013047067407E-4	0.0	0.0	0.04528281205577801	0.0
27	1.37013047067407E-4	0.0	0.0	0.12276369017239666	0.0
28	1.37013047067407E-4	0.0	0.0	0.1997650226242794	0.0
29	1.37013047067407E-4	0.0	0.0	0.29855142955987984	0.0
30	1.37013047067407E-4	0.0	0.0	0.49680930866641776	0.0
31	1.37013047067407E-4	0.0	0.0	0.7714519615130351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5570	0.0	31.420109	1
GTATTGG	355	0.0	26.056337	1
GTCCTAC	85	1.9772415E-9	23.941175	30
TGCGACG	85	1.9772415E-9	23.941175	22
CAATGCG	85	1.9772415E-9	23.941175	19
TCACGTT	70	1.9236722E-7	23.785713	24
ATTGGAC	385	0.0	23.545454	3
ATCGTCA	95	2.8194336E-10	23.368422	10
TATTGGA	415	0.0	22.734938	2
CGTCTGT	565	0.0	22.265488	34
CGCGTCA	50	2.7025875E-4	22.199999	32
CGACGAG	95	7.1486284E-9	21.421053	24
CACGGCT	105	9.840733E-10	21.142857	4
ATCGCAG	70	5.103482E-6	21.142857	11
GTAGACC	80	6.962764E-7	20.8125	3
CGTGTCT	170	0.0	20.67647	35
ACCGTAC	45	0.0038262955	20.555555	8
TCCACGG	135	1.8189894E-12	20.555553	2
CTAGTAC	100	1.290573E-8	20.349998	3
ATTCGCG	65	6.903762E-5	19.923077	29
>>END_MODULE