FastQCFastQC Report
Wed 25 May 2016
SRR2088205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645602
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT202971.2334088072328546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200191.216515293491379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165301.0044956192323538No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76200.4630524270145515No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68050.41352647845590856No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT51410.31240846814721906No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48290.2934488412143398No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT43210.2625786794133697No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39880.24234292374462355No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37000.224841729652735No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34780.21135122587357089No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG32870.19974453118068647No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32540.19773918602432422No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28420.172702755587317No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27680.1682059209942623No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT26200.1592122518081529No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25390.15429004096980922No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25180.15301391223394237No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.15149471135791037No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.12961821874304966No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA19260.11703923548950475No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC18720.11375776159727564No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG18000.10938246307430351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA401.6006234E-632.37514
GGTATCA42550.031.8260861
ATTGGAC3200.019.656253
TGCGCGA701.2196037E-418.512
GTATAGA902.1533651E-618.51
GTATCAA74100.018.3751682
GTATTGG3050.018.1967221
CGTCTGT5700.017.52631634
TTAGTAC953.6083002E-617.5263163
ACGTGCG752.0679497E-417.2666669
ACCGTCG752.0679497E-417.26666623
CGATCTG1201.0419717E-716.95833433
ATAACAC1005.8808946E-616.653
GACCGTC904.447833E-516.44444522
GGCGTCG904.447833E-516.4444458
ATCGGGA1251.658882E-716.27999922
GCGATAT803.383676E-416.187515
ACGGCTC1504.6748028E-916.0333335
CTTAACG700.002593132215.85714230
AGCCGTC1059.347921E-615.85714212
Produced by FastQC (version 0.11.5)