##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088205_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1645602
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084784170169943	31.0	31.0	33.0	28.0	34.0
2	31.248871233749107	31.0	31.0	34.0	28.0	34.0
3	31.301622141927393	31.0	31.0	34.0	28.0	34.0
4	35.25543600457462	37.0	35.0	37.0	33.0	37.0
5	35.108134287634556	37.0	35.0	37.0	32.0	37.0
6	35.15591133214471	37.0	35.0	37.0	32.0	37.0
7	34.989358909383924	37.0	35.0	37.0	32.0	37.0
8	35.03952717607295	37.0	35.0	37.0	32.0	37.0
9	36.538975402314776	38.0	35.0	39.0	32.0	39.0
10	36.389332900664925	38.0	35.0	39.0	32.0	39.0
11	36.42121606561003	38.0	35.0	39.0	32.0	39.0
12	36.251058275330244	38.0	35.0	39.0	32.0	39.0
13	36.306574736783254	38.0	35.0	39.0	32.0	39.0
14	37.224821676201174	39.0	36.0	41.0	32.0	41.0
15	37.29813101831427	39.0	36.0	41.0	32.0	41.0
16	37.27137181408384	39.0	36.0	41.0	32.0	41.0
17	37.35915670982413	39.0	36.0	41.0	32.0	41.0
18	37.38007367516568	39.0	36.0	41.0	32.0	41.0
19	37.47719740253111	39.0	36.0	41.0	32.0	41.0
20	37.43861152332095	39.0	36.0	41.0	32.0	41.0
21	37.31244675200929	39.0	36.0	41.0	31.0	41.0
22	37.27053017679852	39.0	36.0	41.0	31.0	41.0
23	37.15816582624474	39.0	36.0	40.0	31.0	41.0
24	37.06779889669556	39.0	36.0	40.0	31.0	41.0
25	36.98464695594682	39.0	36.0	40.0	31.0	41.0
26	36.715622003376275	39.0	35.0	40.0	30.0	41.0
27	36.562221606439465	38.0	35.0	40.0	30.0	41.0
28	36.42218956953139	38.0	35.0	40.0	30.0	41.0
29	36.272705672453	38.0	35.0	40.0	30.0	41.0
30	36.0970113065006	38.0	35.0	40.0	30.0	41.0
31	35.83629516736124	38.0	34.0	40.0	29.0	41.0
32	35.52493798622024	38.0	34.0	40.0	27.0	41.0
33	35.68358266458111	38.0	34.0	40.0	29.0	41.0
34	35.66302179992489	38.0	34.0	40.0	28.0	41.0
35	35.57831723588085	38.0	34.0	40.0	27.0	41.0
36	35.4879302528801	38.0	34.0	40.0	27.0	41.0
37	35.35881276274579	38.0	34.0	40.0	27.0	41.0
38	35.238177274942544	38.0	34.0	40.0	26.0	41.0
39	35.173556546479645	38.0	34.0	40.0	25.0	41.0
40	35.01579786606968	38.0	34.0	40.0	25.0	41.0
41	34.87108000597957	38.0	33.0	40.0	24.0	41.0
42	34.65184108915764	38.0	33.0	40.0	23.0	41.0
43	34.329260659624865	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	14.0
17	49.0
18	116.0
19	299.0
20	592.0
21	1200.0
22	2244.0
23	3681.0
24	6283.0
25	9794.0
26	14666.0
27	21620.0
28	30342.0
29	40888.0
30	52471.0
31	63979.0
32	78922.0
33	95649.0
34	117230.0
35	140368.0
36	175115.0
37	227687.0
38	280403.0
39	281980.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09851713840892	23.025008477140886	14.268699235902726	22.607775148547464
2	17.308984797052993	23.62041368447535	36.15005329356673	22.92054822490493
3	19.70385305802983	25.714844780208097	32.69861120732717	21.88269095443491
4	13.338097547280569	16.313361310936667	35.60216868963455	34.74637245214821
5	11.45392385279065	40.36759799757171	34.79887603442388	13.379602115213766
6	31.527185795836417	36.41785802399365	15.558500779653889	16.49645540051604
7	24.313290820016018	35.44708866420921	21.730588562726588	18.509031953048186
8	27.105278189987615	33.9053428471769	20.594651683699947	18.39472727913554
9	25.103214507517613	14.006120556489357	24.207615207079233	36.683049728913794
10	16.146249214573146	28.420055396140743	34.35770010002418	21.075995289261922
11	31.394772247481466	22.35789698845772	25.014797016532555	21.232533747528258
12	20.012736980144652	27.96271516442007	31.73385788301181	20.290689972423465
13	30.431173515831894	21.566879476325383	28.75233501174646	19.24961199609626
14	20.711812455259533	23.064203859742513	29.027857282623625	27.196126402374325
15	22.77895870325875	29.841723575931482	26.743951453632164	20.635366267177606
16	21.596959653670815	28.596586537935657	27.64495910918922	22.161494699204304
17	19.121512978229244	28.014246458135077	28.60837553673367	24.25586502690201
18	19.975425406629306	27.15814638047353	32.63760010014572	20.228828112751444
19	19.558556686246128	25.906264090588127	33.9935172660218	20.54166195714395
20	23.182033079687557	25.088751715177786	32.67788930737809	19.051325897756566
21	22.735144949993984	25.740610427065597	31.422543239495333	20.101701383445086
22	21.134028762726345	25.784728020505565	32.12581170902806	20.955431507740023
23	20.579338138869545	27.29536060359674	32.81972190116444	19.30557935636928
24	19.367076607831056	26.70135306106823	31.583760836459852	22.347809494640867
25	20.396061745185044	27.404439226495835	32.524632323003985	19.674866705315136
26	21.359964317009826	26.522087357696456	31.472494564299264	20.645453760994457
27	20.198747935406008	27.7597499273822	31.074342398708797	20.967159738502993
28	20.258908290096876	27.41312905550674	31.18020031575071	21.147762338645677
29	19.27464842653327	27.366641508700155	31.96666022525495	21.39204983951162
30	18.8420407850744	27.534786661659382	33.73835228688346	19.884820266382757
31	20.27665255632893	26.895810773200324	32.52997991008761	20.29755676038313
32	20.82083031012359	27.243950845951815	31.057388116932284	20.87783072699231
33	21.586993695924043	27.13456838287751	30.623504346737544	20.654933574460895
34	20.256599104765307	29.294628956454844	30.56990693983114	19.878864998948714
35	19.05673425287524	30.032535205961103	30.80933299789378	20.10139754326988
36	19.980955297818063	27.057149906234923	32.44417544460933	20.51771935133769
37	19.853220888161292	27.10673662282861	31.89574392836178	21.144298560648323
38	20.1061374500031	26.896236149445613	32.446849238151145	20.550777162400145
39	20.9988198847595	26.30599622508966	32.299425985141	20.395757905009837
40	19.40280821243533	26.898970711022468	31.95882114873463	21.739399927807572
41	20.027442844624645	26.38140935657589	31.69958471124853	21.89156308755094
42	17.930398723385117	27.72492984330355	32.142279846524254	22.202391586787083
43	18.41295768964792	27.43129869798408	31.860559236072877	22.29518437629512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	293.0
2	367.0
3	880.0
4	1393.0
5	1393.0
6	2376.0
7	3359.0
8	3585.0
9	3811.0
10	5682.0
11	7553.0
12	7553.0
13	13529.0
14	19505.0
15	31182.0
16	42859.0
17	42251.0
18	41643.0
19	41643.0
20	45249.5
21	48856.0
22	40815.5
23	32775.0
24	35894.0
25	39013.0
26	39013.0
27	43244.5
28	47476.0
29	49409.0
30	51342.0
31	55262.0
32	59182.0
33	59182.0
34	63971.0
35	68760.0
36	73604.0
37	78448.0
38	80581.0
39	82714.0
40	82714.0
41	86071.0
42	89428.0
43	97908.0
44	106388.0
45	129351.0
46	152314.0
47	152314.0
48	154048.5
49	155783.0
50	142100.5
51	128418.0
52	118799.5
53	109181.0
54	109181.0
55	96380.5
56	83580.0
57	69140.5
58	54701.0
59	47766.0
60	40831.0
61	40831.0
62	35651.0
63	30471.0
64	26261.0
65	22051.0
66	18815.5
67	15580.0
68	15580.0
69	13097.0
70	10614.0
71	8981.0
72	7348.0
73	5739.5
74	4131.0
75	4131.0
76	3286.5
77	2442.0
78	1933.5
79	1425.0
80	1143.5
81	862.0
82	862.0
83	623.0
84	384.0
85	297.5
86	211.0
87	160.5
88	110.0
89	110.0
90	82.0
91	54.0
92	35.5
93	17.0
94	10.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1645602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.41810783716578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67343813035585	29.978335198366977
2	14.483575831641712	12.287317629886923
3	5.907113560830533	7.517057400890816
4	2.9966437790738643	5.084478358813086
5	1.6889055024926138	3.5820087865757175
6	1.0299127189199075	2.6212169264407947
7	0.6392521379155487	1.8981106284868356
8	0.477030118048807	1.6187771991176172
9	0.3313796403232685	1.265084758644625
>10	1.553326079117342	11.766558002716888
>50	0.129229728070118	3.772026161542294
>100	0.07374175220532116	5.961081218400604
>500	0.008658432108055872	2.4950780036553977
>1k	0.006926745686444699	5.475509003826672
>5k	4.3292160540279366E-4	1.1976815146714141
>10k+	4.3292160540279366E-4	3.4796792079633656
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20297	1.2334088072328546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20019	1.216515293491379	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16530	1.0044956192323538	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7620	0.4630524270145515	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6805	0.41352647845590856	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5141	0.31240846814721906	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4829	0.2934488412143398	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4321	0.2625786794133697	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3988	0.24234292374462355	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3700	0.224841729652735	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3478	0.21135122587357089	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3287	0.19974453118068647	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3254	0.19773918602432422	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2842	0.172702755587317	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2768	0.1682059209942623	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2620	0.1592122518081529	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2539	0.15429004096980922	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2518	0.15301391223394237	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2493	0.15149471135791037	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2133	0.12961821874304966	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1926	0.11703923548950475	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1872	0.11375776159727564	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1800	0.10938246307430351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.0768035041279724E-5	0.0
10	0.0	0.0	0.0	6.0768035041279724E-5	0.0
11	0.0	0.0	0.0	1.2153607008255945E-4	0.0
12	0.0	0.0	0.0	1.8230410512383918E-4	0.0
13	0.0	0.0	0.0	2.430721401651189E-4	0.0
14	0.0	0.0	0.0	5.469123153715175E-4	0.0
15	0.0	0.0	0.0	8.507524905779162E-4	0.0
16	0.0	0.0	0.0	0.0013976648059494338	0.0
17	0.0	6.0768035041279724E-5	0.0	0.0024914894366924686	0.0
18	0.0	6.0768035041279724E-5	0.0	0.0026130255067750283	0.0
19	0.0	6.0768035041279724E-5	0.0	0.003038401752063986	0.0
20	0.0	6.0768035041279724E-5	0.0	0.0033422419272703847	0.0
21	0.0	6.0768035041279724E-5	0.0	0.004253762452889581	0.0
22	0.0	6.0768035041279724E-5	0.0	0.0066237158194994905	0.0
23	0.0	6.0768035041279724E-5	0.0	0.010695174167265231	0.0
24	0.0	6.0768035041279724E-5	0.0	0.01823041051238392	0.0
25	0.0	6.0768035041279724E-5	0.0	0.023334925455851415	0.0
26	0.0	6.0768035041279724E-5	0.0	0.032753970887249774	0.0
27	0.0	6.0768035041279724E-5	0.0	0.1109016639503355	0.0
28	0.0	6.0768035041279724E-5	0.0	0.19287774322102186	0.0
29	0.0	6.0768035041279724E-5	0.0	0.28220675473170304	0.0
30	0.0	6.0768035041279724E-5	0.0	0.4435458877663007	0.0
31	0.0	6.0768035041279724E-5	0.0	0.7029038613224826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	40	1.6006234E-6	32.375	14
GGTATCA	4255	0.0	31.826086	1
ATTGGAC	320	0.0	19.65625	3
TGCGCGA	70	1.2196037E-4	18.5	12
GTATAGA	90	2.1533651E-6	18.5	1
GTATCAA	7410	0.0	18.375168	2
GTATTGG	305	0.0	18.196722	1
CGTCTGT	570	0.0	17.526316	34
TTAGTAC	95	3.6083002E-6	17.526316	3
ACGTGCG	75	2.0679497E-4	17.266666	9
ACCGTCG	75	2.0679497E-4	17.266666	23
CGATCTG	120	1.0419717E-7	16.958334	33
ATAACAC	100	5.8808946E-6	16.65	3
GACCGTC	90	4.447833E-5	16.444445	22
GGCGTCG	90	4.447833E-5	16.444445	8
ATCGGGA	125	1.658882E-7	16.279999	22
GCGATAT	80	3.383676E-4	16.1875	15
ACGGCTC	150	4.6748028E-9	16.033333	5
CTTAACG	70	0.0025931322	15.857142	30
AGCCGTC	105	9.347921E-6	15.857142	12
>>END_MODULE