FastQCFastQC Report
Wed 25 May 2016
SRR2088204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419700
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT401661.6599578460139688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT400331.654461296854982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT343351.4189775592015539No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG176340.7287680290945158No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.5502335000206637No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT112060.46311526222258964No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA106900.4417903045832128No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97290.4020746373517378No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87340.360953837252552No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA84840.35062197793114847No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT81910.3385130388064636No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT75190.3107410009505311No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG73420.3034260445509774No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA66300.27400090920362025No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA62780.25945365127908415No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT60420.2497003760796793No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54080.2234987808406001No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.20659585899078398No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA47190.19502417655081208No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45740.18903169814439807No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC44150.18246063561598547No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39260.16225151878332025No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38490.15906930611232797No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36370.15030788940777784No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35190.14543125180807537No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32770.1354300119849568No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC32700.13514071992395751No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA31920.13191717981567963No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC31500.13018142744968386No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31410.12980948051411334No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG29900.12356903748398562No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC29290.12104806380956318No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC29040.12001487787742281No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA28650.11840310782328387No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA28650.11840310782328387No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28390.11732859445385792No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT26270.10856717774930776No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC25160.10397983221060463No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC25020.10340124808860603No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA24960.10315328346489235No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG24900.10290531884117866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC24620.10174815059718148No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA24580.10158284084803902No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA24560.1015001859734678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82950.029.9969861
GTATTGG5850.023.401711
GTCGTCA603.728479E-521.58333224
ATTGGAC6250.021.3123
ACGGACC450.003827011220.5555578
GGACCCT14300.019.017486
CGTCTGT9650.018.97927534
TGCGTCG500.007037073418.510
TTGGACC14400.018.3715294
GACCCTC13700.018.3649647
TGGACCC15300.017.532685
GAGCACG752.0684558E-417.2666669
GTTATCA2900.017.2241381
TGCGACG650.001580616717.07692322
GCGCGTG1201.042572E-716.9583328
GTATCAA147400.016.8934882
TTTTTAC23100.016.417751
TATTGGA8400.016.2976192
AACGGTA803.3844975E-416.187518
CGTTTAG1504.6784407E-916.03333326
Produced by FastQC (version 0.11.5)