##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088204_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2419700
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.076418151010454	31.0	31.0	33.0	30.0	34.0
2	31.218673802537506	31.0	31.0	34.0	28.0	34.0
3	31.246674794396	31.0	31.0	34.0	28.0	34.0
4	35.21497127743109	37.0	35.0	37.0	33.0	37.0
5	35.0988581229078	35.0	35.0	37.0	32.0	37.0
6	35.15724593957928	37.0	35.0	37.0	32.0	37.0
7	34.97911724593958	37.0	35.0	37.0	32.0	37.0
8	35.049150721163784	37.0	35.0	37.0	32.0	37.0
9	36.49728354754722	38.0	35.0	39.0	32.0	39.0
10	36.37782245732942	38.0	35.0	39.0	32.0	39.0
11	36.42051907261231	38.0	35.0	39.0	32.0	39.0
12	36.26018184072406	38.0	35.0	39.0	32.0	39.0
13	36.309778898210524	38.0	35.0	39.0	32.0	39.0
14	37.189537545976776	39.0	36.0	41.0	31.0	41.0
15	37.25120138860189	39.0	36.0	41.0	32.0	41.0
16	37.218951109641694	39.0	36.0	41.0	32.0	41.0
17	37.35663346695871	39.0	36.0	41.0	32.0	41.0
18	37.397597636070586	39.0	36.0	41.0	32.0	41.0
19	37.50581931644419	39.0	36.0	41.0	32.0	41.0
20	37.474246394181094	39.0	36.0	41.0	32.0	41.0
21	37.35065999917345	39.0	36.0	41.0	31.0	41.0
22	37.31491465884201	39.0	36.0	41.0	31.0	41.0
23	37.19494152167624	39.0	36.0	41.0	31.0	41.0
24	37.09456420217382	39.0	36.0	41.0	31.0	41.0
25	37.017686903335125	39.0	35.0	40.0	31.0	41.0
26	36.726152828863086	39.0	35.0	40.0	30.0	41.0
27	36.55420010745134	38.0	35.0	40.0	30.0	41.0
28	36.38490267388519	38.0	35.0	40.0	30.0	41.0
29	36.22623217754267	38.0	35.0	40.0	30.0	41.0
30	36.038694466256146	38.0	35.0	40.0	30.0	41.0
31	35.72198206389222	38.0	34.0	40.0	29.0	41.0
32	35.414364590651736	38.0	34.0	40.0	27.0	41.0
33	35.47779766086705	38.0	34.0	40.0	28.0	41.0
34	35.41561061288589	38.0	34.0	40.0	27.0	41.0
35	35.29008761416704	38.0	34.0	40.0	27.0	41.0
36	35.17409430921189	38.0	34.0	40.0	26.0	41.0
37	35.0067371988263	38.0	34.0	40.0	25.0	41.0
38	34.84354548084473	38.0	33.0	40.0	24.0	41.0
39	34.71553828987064	38.0	33.0	40.0	24.0	41.0
40	34.48877918750258	38.0	33.0	40.0	23.0	41.0
41	34.30189155680456	38.0	33.0	40.0	21.0	41.0
42	34.025804438566766	38.0	33.0	40.0	19.0	41.0
43	33.68330247551349	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	1.0
15	10.0
16	29.0
17	71.0
18	214.0
19	477.0
20	1021.0
21	1994.0
22	3606.0
23	6246.0
24	10072.0
25	15715.0
26	24038.0
27	34542.0
28	48391.0
29	64367.0
30	80818.0
31	98013.0
32	118720.0
33	143312.0
34	172075.0
35	205329.0
36	256097.0
37	338007.0
38	395215.0
39	401314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.25660205810638	23.44898127867091	14.944125304789848	21.350291358432862
2	17.070917882382115	23.566971112121337	36.996652477579865	22.365458527916683
3	19.906682646609084	26.21614249700376	33.243542587924125	20.633632268463032
4	12.544571641112533	15.76228458073315	35.17320328966401	36.51994048849031
5	10.371037731950242	41.66805802372195	34.40786047857172	13.553043765756085
6	30.830474852254415	35.985246104889036	15.416580567838988	17.767698475017564
7	23.35136587180229	36.92763565731289	21.113981072033724	18.6070173988511
8	28.146464437740214	33.38161755589536	20.201305947018223	18.2706120593462
9	24.89077158325412	13.778980865396537	25.355705252717282	35.97454229863206
10	16.315493656238377	28.65636235897012	33.49601190230194	21.532132082489564
11	30.10732735463074	22.124023639294126	26.056411951894866	21.71223705418027
12	20.19382568086953	28.519857833615735	31.872752820597594	19.413563664917138
13	31.124436913666987	21.494937388932513	30.05322973922387	17.327395958176634
14	20.560028102657355	23.468653138818862	29.78422944993181	26.187089308591975
15	22.323717816258217	29.673554572880935	28.355209323469854	19.647518287391
16	20.763524403851715	28.860023969913623	28.482373847997682	21.894077778236973
17	18.01901062115138	27.87804273257015	28.947844774145555	25.155101872132906
18	19.194528247303385	26.909451584907217	34.58606438814729	19.309955779642106
19	18.287680290945158	25.203165681696078	36.3950076455759	20.114146381782867
20	23.68235731702277	23.707360416580567	34.77550936066455	17.834772905732116
21	22.881927511675002	24.923089639211472	33.085175848245655	19.109807000867875
22	20.594040583543414	24.699962805306445	34.11311319585072	20.59288341529942
23	19.56081332396578	27.136008596106954	35.30470719510683	17.998470884820435
24	17.687895193619045	26.220399223044176	33.53630615365541	22.555399429681366
25	19.281398520477744	27.259164359218087	34.99123858329545	18.46819853700872
26	20.989213538868455	25.852874323263215	33.29602843327685	19.86188370459148
27	19.350911269992146	27.674835723436793	32.70760838120428	20.26664462536678
28	19.0235566392528	27.11803116088771	32.884572467661286	20.973839732198208
29	17.817332727197584	27.16266479315618	33.99173451254288	21.02826796710336
30	17.16365665165103	27.135636649171385	36.7368682068025	18.96383849237509
31	19.010249204446833	26.522254824978305	34.91424556763235	19.553250402942513
32	20.415010125222135	26.87242220109931	32.501880398396494	20.210687275282062
33	21.53056163987271	26.834524941108402	31.71178245237013	19.92313096664876
34	19.23668223333471	30.147580278546926	31.880109104434435	18.735628383683927
35	17.56440881100963	31.198206389221806	32.102368062156465	19.1350167376121
36	18.755920155391166	26.736620242178784	34.78757697235195	19.719882630078107
37	18.8463032607348	27.010207877009545	33.63462412695789	20.508864735297763
38	19.334380295077903	26.633921560524033	34.29325949497872	19.738438649419347
39	20.629086250361613	25.92466008182833	33.97123610364921	19.475017564160847
40	18.44889862379634	26.80770343431004	33.12080009918585	21.622597842707776
41	19.408397735256436	26.264495598627928	32.899243707897675	21.42786295821796
42	16.581642352357733	28.125842046534693	33.41364631979171	21.878869281315865
43	17.278298962681323	27.915981319998345	33.00252097367442	21.803198743645908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	323.0
2	456.0
3	1308.5
4	2161.0
5	2161.0
6	3891.5
7	5622.0
8	6323.0
9	7024.0
10	10644.0
11	14264.0
12	14264.0
13	25574.5
14	36885.0
15	60565.0
16	84245.0
17	82060.5
18	79876.0
19	79876.0
20	84356.0
21	88836.0
22	70879.0
23	52922.0
24	55917.5
25	58913.0
26	58913.0
27	63144.0
28	67375.0
29	67580.5
30	67786.0
31	72262.5
32	76739.0
33	76739.0
34	82252.0
35	87765.0
36	94076.0
37	100387.0
38	102151.5
39	103916.0
40	103916.0
41	109411.5
42	114907.0
43	135364.5
44	155822.0
45	208802.0
46	261782.0
47	261782.0
48	264390.0
49	266998.0
50	237839.0
51	208680.0
52	188713.0
53	168746.0
54	168746.0
55	142939.5
56	117133.0
57	90461.0
58	63789.0
59	52845.5
60	41902.0
61	41902.0
62	35703.5
63	29505.0
64	24663.0
65	19821.0
66	16495.0
67	13169.0
68	13169.0
69	11182.5
70	9196.0
71	7374.5
72	5553.0
73	4385.5
74	3218.0
75	3218.0
76	2519.0
77	1820.0
78	1409.5
79	999.0
80	808.5
81	618.0
82	618.0
83	469.0
84	320.0
85	243.0
86	166.0
87	140.0
88	114.0
89	114.0
90	89.0
91	64.0
92	38.0
93	12.0
94	7.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2419700.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.29909875830914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.2510191000724	23.059965241268635
2	14.443208159466678	9.618916297777947
3	5.948824178277476	5.942714514248367
4	3.0924634792248944	4.119049872046962
5	1.84403123027479	3.0702289025163267
6	1.1437830935099753	2.2852167713323803
7	0.8084621488667667	1.8844742656228504
8	0.5503085258549443	1.4659822359986647
9	0.40448009856224054	1.2121940473015198
>10	2.151769608344019	13.43738065123265
>50	0.20616218170577746	4.76953908879093
>100	0.12805111206379285	8.04324839234423
>500	0.013405333169286632	2.9655851012771897
>1k	0.011901931318525514	8.040738075501002
>5k	0.0012528348756342645	3.102045569812171
>10k+	8.769844129439852E-4	6.982720972928193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40166	1.6599578460139688	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40033	1.654461296854982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34335	1.4189775592015539	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17634	0.7287680290945158	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13314	0.5502335000206637	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11206	0.46311526222258964	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10690	0.4417903045832128	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9729	0.4020746373517378	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8734	0.360953837252552	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8484	0.35062197793114847	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8191	0.3385130388064636	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7519	0.3107410009505311	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7342	0.3034260445509774	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6630	0.27400090920362025	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6278	0.25945365127908415	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6042	0.2497003760796793	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5408	0.2234987808406001	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4999	0.20659585899078398	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4719	0.19502417655081208	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4574	0.18903169814439807	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4415	0.18246063561598547	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3926	0.16225151878332025	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3849	0.15906930611232797	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3637	0.15030788940777784	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3519	0.14543125180807537	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3277	0.1354300119849568	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3270	0.13514071992395751	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3192	0.13191717981567963	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3150	0.13018142744968386	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3141	0.12980948051411334	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2990	0.12356903748398562	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2929	0.12104806380956318	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2904	0.12001487787742281	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2865	0.11840310782328387	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	2865	0.11840310782328387	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2839	0.11732859445385792	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	2627	0.10856717774930776	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2516	0.10397983221060463	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2502	0.10340124808860603	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2496	0.10315328346489235	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	2490	0.10290531884117866	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2462	0.10174815059718148	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2458	0.10158284084803902	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2456	0.1015001859734678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.132743728561392E-5	0.0	0.0	0.0	0.0
6	1.2398231185684175E-4	0.0	0.0	0.0	0.0
7	1.2398231185684175E-4	0.0	0.0	4.132743728561392E-5	0.0
8	1.2398231185684175E-4	0.0	0.0	4.132743728561392E-5	0.0
9	1.2398231185684175E-4	0.0	0.0	4.132743728561392E-5	0.0
10	1.2398231185684175E-4	0.0	0.0	1.2398231185684175E-4	0.0
11	1.2398231185684175E-4	0.0	0.0	2.066371864280696E-4	0.0
12	1.6530974914245568E-4	0.0	0.0	2.8929206099929743E-4	0.0
13	1.6530974914245568E-4	0.0	0.0	3.3061949828491137E-4	0.0
14	2.066371864280696E-4	0.0	0.0	5.372566847129809E-4	0.0
15	2.066371864280696E-4	0.0	0.0	0.001115840806711576	0.0
16	2.066371864280696E-4	0.0	0.0	0.0019423895524238542	0.0
17	2.066371864280696E-4	0.0	0.0	0.003223540108277886	0.0
18	2.066371864280696E-4	0.0	0.0	0.003760796792990867	0.0
19	2.066371864280696E-4	0.0	0.0	0.004463363226846304	0.0
20	2.066371864280696E-4	0.0	0.0	0.005744513782700335	0.0
21	2.066371864280696E-4	0.0	0.0	0.007769558209695417	0.0
22	2.066371864280696E-4	0.0	0.0	0.012108939124684878	0.0
23	2.066371864280696E-4	0.0	0.0	0.020002479646237137	0.0
24	2.066371864280696E-4	0.0	0.0	0.03215274620820763	0.0
25	2.066371864280696E-4	0.0	0.0	0.03913708310947638	0.0
26	2.066371864280696E-4	0.0	0.0	0.04975823449187916	0.0
27	2.066371864280696E-4	0.0	0.0	0.14055461420837295	0.0
28	2.066371864280696E-4	0.0	0.0	0.23548373765342812	0.0
29	2.066371864280696E-4	0.0	0.0	0.3408687027317436	0.0
30	2.066371864280696E-4	0.0	0.0	0.5558953589287928	0.0
31	2.066371864280696E-4	0.0	0.0	0.8709344133570277	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8295	0.0	29.996986	1
GTATTGG	585	0.0	23.40171	1
GTCGTCA	60	3.728479E-5	21.583332	24
ATTGGAC	625	0.0	21.312	3
ACGGACC	45	0.0038270112	20.555557	8
GGACCCT	1430	0.0	19.01748	6
CGTCTGT	965	0.0	18.979275	34
TGCGTCG	50	0.0070370734	18.5	10
TTGGACC	1440	0.0	18.371529	4
GACCCTC	1370	0.0	18.364964	7
TGGACCC	1530	0.0	17.53268	5
GAGCACG	75	2.0684558E-4	17.266666	9
GTTATCA	290	0.0	17.224138	1
TGCGACG	65	0.0015806167	17.076923	22
GCGCGTG	120	1.042572E-7	16.958332	8
GTATCAA	14740	0.0	16.893488	2
TTTTTAC	2310	0.0	16.41775	1
TATTGGA	840	0.0	16.297619	2
AACGGTA	80	3.3844975E-4	16.1875	18
CGTTTAG	150	4.6784407E-9	16.033333	26
>>END_MODULE