FastQCFastQC Report
Wed 25 May 2016
SRR2088203_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088203_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043699
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT494091.6233208342874905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT486951.599862535684376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426441.4010583832369758No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG258640.8497555113038444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171330.5629006021948951No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT142930.4695930839416118No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA134300.44123942610619515No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT131800.4330257361191104No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA130720.4294774220446897No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA111920.36771047334181206No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT108060.3550285360017531No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT103390.3396853631058787No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG101060.3320302040379157No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA84870.2788383476815546No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA81510.2677991483389126No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT75910.24940048276784268No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA72460.23806559058566568No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA63110.20734639003396854No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT59670.19604435261173983No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC56970.18717356742568828No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52980.17406451820630095No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG48640.15980555238872177No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC48090.1579985405915631No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG45650.14998197916416833No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44960.14771500072773294No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT44910.14755072692799123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.13089336363418327No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC39020.12819927331841946No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC37590.12350104264580695No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA37330.12264681888715015No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37110.12192401416828667No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG36780.1208398070899915No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC35780.11755433109515757No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA33740.11085196006569636No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA33340.10953776966776281No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC33290.10937349586802113No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC33270.10930778634812444No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA32350.10628514843287723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30640.10066698448171123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.029.278111
GTATTGG9200.024.7336961
ATTGGAC9500.022.9789473
TTGGACC18750.020.6213344
ACGCATA656.9069116E-519.9230777
GGACCCT18750.019.6346686
TATTGGA12150.018.5761322
CTAGACG500.00703748218.54
ATCACGT2100.018.523
TGGACCC20600.018.1407785
GACCCTC20350.017.5454547
TTTTTAC24100.017.1950231
CGTGTCT3700.017.035
TATACTG4150.016.9397585
CGTCTGT11900.016.78991534
GTATCAA188750.016.7700652
ACGTTTA2350.016.53191626
CTAGCGG803.384857E-416.187529
GTTATCA4150.016.0481931
CGAACGA700.002593810715.85714216
Produced by FastQC (version 0.11.5)