##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3043699
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067070364053738	31.0	31.0	33.0	30.0	34.0
2	31.19778006957981	31.0	31.0	34.0	28.0	34.0
3	31.236915673987475	31.0	31.0	34.0	28.0	34.0
4	35.199470775526756	37.0	35.0	37.0	33.0	37.0
5	35.08068044836234	35.0	35.0	37.0	32.0	37.0
6	35.141983487854745	37.0	35.0	37.0	32.0	37.0
7	34.97056804894308	37.0	35.0	37.0	32.0	37.0
8	35.05129876508814	37.0	35.0	37.0	32.0	37.0
9	36.50745260947288	38.0	35.0	39.0	32.0	39.0
10	36.38586272821327	38.0	35.0	39.0	32.0	39.0
11	36.4106483591183	38.0	35.0	39.0	32.0	39.0
12	36.25360063527964	38.0	35.0	39.0	32.0	39.0
13	36.313337816912906	38.0	35.0	39.0	32.0	39.0
14	37.20859158543601	39.0	36.0	41.0	31.0	41.0
15	37.255160579281984	39.0	36.0	41.0	32.0	41.0
16	37.21869015300133	39.0	36.0	41.0	32.0	41.0
17	37.35224968040532	39.0	36.0	41.0	32.0	41.0
18	37.371193406443936	39.0	36.0	41.0	32.0	41.0
19	37.4779319505641	39.0	36.0	41.0	32.0	41.0
20	37.440749233087764	39.0	36.0	41.0	32.0	41.0
21	37.322873582440316	39.0	36.0	41.0	31.0	41.0
22	37.28043081789625	39.0	36.0	41.0	31.0	41.0
23	37.16093805596414	39.0	36.0	40.0	31.0	41.0
24	37.071676601398494	39.0	36.0	40.0	31.0	41.0
25	36.99452442570701	39.0	35.0	40.0	31.0	41.0
26	36.69819486092416	38.0	35.0	40.0	30.0	41.0
27	36.52126540765036	38.0	35.0	40.0	30.0	41.0
28	36.34397783749313	38.0	35.0	40.0	30.0	41.0
29	36.19283181418399	38.0	35.0	40.0	30.0	41.0
30	36.000834839450285	38.0	35.0	40.0	30.0	41.0
31	35.69367371740767	38.0	34.0	40.0	29.0	41.0
32	35.42366344372423	38.0	34.0	40.0	27.0	41.0
33	35.50865345094899	38.0	34.0	40.0	28.0	41.0
34	35.466621042356685	38.0	34.0	40.0	27.0	41.0
35	35.34283613458492	38.0	34.0	40.0	27.0	41.0
36	35.22768282934679	38.0	34.0	40.0	26.0	41.0
37	35.065515348265386	38.0	34.0	40.0	25.0	41.0
38	34.91093895947004	38.0	34.0	40.0	25.0	41.0
39	34.792109535141286	38.0	33.0	40.0	24.0	41.0
40	34.56873429337132	38.0	33.0	40.0	23.0	41.0
41	34.38782941414377	38.0	33.0	40.0	22.0	41.0
42	34.12633575133415	38.0	33.0	40.0	21.0	41.0
43	33.78380582311194	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	7.0
15	8.0
16	39.0
17	124.0
18	216.0
19	622.0
20	1194.0
21	2417.0
22	4356.0
23	7747.0
24	12481.0
25	19550.0
26	30000.0
27	43225.0
28	60345.0
29	80555.0
30	101827.0
31	123263.0
32	148641.0
33	179675.0
34	216738.0
35	259159.0
36	324074.0
37	422548.0
38	507170.0
39	497716.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.871189956694145	23.19503341164813	14.43132188826819	21.50245474338954
2	17.32270503752178	23.517437171021182	36.694627162541366	22.465230628915673
3	20.200223478077167	26.420220921976846	32.859392469491894	20.520163130454094
4	12.608934063453711	15.542338450681225	35.123183994212305	36.72554349165276
5	10.458097203435686	41.56274979884673	34.41831797428064	13.560835023436942
6	31.077941675573044	35.689567200961726	15.426689695663073	17.805801427802155
7	23.53393026051525	36.53518301251208	21.107277690730918	18.823609036241756
8	28.347349721506625	33.01288990797053	20.345737209888362	18.294023160634477
9	25.067524745383825	13.40799468015727	24.865467971701538	36.65901260275737
10	16.441901778066754	28.00681013464209	33.44512712985088	22.10616095744027
11	30.496281005447646	21.505937347944066	26.056617293628577	21.941164352979715
12	20.165791689651307	27.837739539947936	32.135799236389666	19.860669534011084
13	31.45393154842184	20.876538711613733	30.013250324687164	17.656279415277265
14	20.686473925312587	22.76417608968561	29.53659346735666	27.012756517645144
15	22.63141000473437	28.842898065807425	28.444928358553195	20.08076357090501
16	21.052771643976623	28.59911574699075	28.225097159738855	22.12301544929377
17	18.3203398233531	26.987031240605592	28.7564243376234	25.936204598417913
18	19.638669921040155	26.271224585611126	34.383689057295086	19.70641643605363
19	18.535472791494822	24.49926881731735	35.957563477860326	21.0076949133275
20	24.225292974108147	23.039268994733053	34.41158274849123	18.32385528266757
21	23.370937796411535	24.30979541669528	32.74788341422723	19.571383372665956
22	21.140395288758842	23.716964128187445	33.99688996842329	21.145750614630423
23	19.87795113774391	26.300169629125612	35.27385592333539	18.548023309795088
24	18.30203972206187	25.234722618760923	33.35707637318933	23.106161285987874
25	19.759969694769424	26.413912808066765	34.812213691301274	19.013903805862537
26	21.623393114759377	25.01860400782075	32.95785818505706	20.40014469236281
27	19.89142158932273	26.939194710120812	32.500585636096076	20.668798064460383
28	19.48057281616875	26.256111396034893	32.5842667096845	21.679049078111863
29	18.36755211339886	26.221515333809286	33.84477242986248	21.56616012292937
30	17.559620711509254	26.32214946353105	36.53623436483043	19.58199546012927
31	19.383946967160682	25.712233699850085	34.83787325882093	20.065946074168306
32	20.954240218891552	25.980295686268583	32.21287650322847	20.85258759161139
33	22.079844294721653	25.935514648458994	31.497004138714114	20.48763691810524
34	19.603679601695173	29.676949001855967	31.607724679740013	19.111646716708847
35	18.010026615641035	30.66025911234981	31.776959548233908	19.552754723775248
36	19.234293535596	26.07110624276579	34.60128613243294	20.093314089205272
37	19.45379618681085	26.586827409674875	33.218724979046875	20.740651424467398
38	19.850977379826322	25.96577388237142	33.956642887486574	20.226605850315686
39	21.222335060069998	25.2994136410992	33.652243536565216	19.826007762265586
40	18.98535301946743	26.428927433363153	32.41920439570404	22.166515151465372
41	20.335190831944946	25.793614940242122	32.20837540111555	21.662818826697382
42	17.305127740949416	27.822954897971186	32.84477210131488	22.027145259764517
43	18.16027143288479	27.624742131202858	32.23265506871738	21.98233136719498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	268.0
1	407.5
2	547.0
3	1507.5
4	2468.0
5	2468.0
6	4451.0
7	6434.0
8	7175.5
9	7917.0
10	12145.0
11	16373.0
12	16373.0
13	30272.0
14	44171.0
15	72688.5
16	101206.0
17	98352.0
18	95498.0
19	95498.0
20	99632.0
21	103766.0
22	81560.0
23	59354.0
24	63603.0
25	67852.0
26	67852.0
27	71918.5
28	75985.0
29	77862.5
30	79740.0
31	84327.5
32	88915.0
33	88915.0
34	95268.5
35	101622.0
36	109504.0
37	117386.0
38	122418.5
39	127451.0
40	127451.0
41	136519.0
42	145587.0
43	170984.5
44	196382.0
45	264614.5
46	332847.0
47	332847.0
48	337906.0
49	342965.0
50	306192.0
51	269419.0
52	246593.5
53	223768.0
54	223768.0
55	190466.0
56	157164.0
57	122855.5
58	88547.0
59	73547.0
60	58547.0
61	58547.0
62	50234.0
63	41921.0
64	36345.0
65	30769.0
66	26155.5
67	21542.0
68	21542.0
69	17899.5
70	14257.0
71	11938.0
72	9619.0
73	7752.5
74	5886.0
75	5886.0
76	4559.0
77	3232.0
78	2520.5
79	1809.0
80	1482.5
81	1156.0
82	1156.0
83	850.0
84	544.0
85	436.5
86	329.0
87	285.0
88	241.0
89	241.0
90	204.5
91	168.0
92	101.0
93	34.0
94	21.0
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3043699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.70016727864178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.11198268029712	24.67597694522187
2	13.853171935465907	9.614147670009054
3	5.761084858892131	5.99731824930022
4	2.8594586935657405	3.968947799723907
5	1.641088500724271	2.8473022747093824
6	1.0362323179784199	2.1574460864031533
7	0.6736535362780248	1.6363123277687228
8	0.47389027449000215	1.3155257437219634
9	0.3504276896302939	1.0943909504315246
>10	1.8923250595864483	12.418026543003148
>50	0.19544973054883708	4.7074560787811475
>100	0.1279527107052012	8.2564757540696
>500	0.011736424000136867	2.8675118004867284
>1k	0.009541808130192226	7.354180255524405
>5k	7.633446504153781E-4	1.8127190814969478
>10k+	0.0012404350569249893	9.276262439348093
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	49409	1.6233208342874905	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48695	1.599862535684376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	42644	1.4010583832369758	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	25864	0.8497555113038444	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17133	0.5629006021948951	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	14293	0.4695930839416118	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13430	0.44123942610619515	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	13180	0.4330257361191104	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13072	0.4294774220446897	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	11192	0.36771047334181206	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	10806	0.3550285360017531	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	10339	0.3396853631058787	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	10106	0.3320302040379157	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8487	0.2788383476815546	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	8151	0.2677991483389126	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	7591	0.24940048276784268	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7246	0.23806559058566568	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	6311	0.20734639003396854	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5967	0.19604435261173983	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5697	0.18717356742568828	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5298	0.17406451820630095	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4864	0.15980555238872177	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4809	0.1579985405915631	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4565	0.14998197916416833	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4496	0.14771500072773294	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4491	0.14755072692799123	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3984	0.13089336363418327	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3902	0.12819927331841946	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3759	0.12350104264580695	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3733	0.12264681888715015	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3711	0.12192401416828667	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3678	0.1208398070899915	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	3578	0.11755433109515757	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	3374	0.11085196006569636	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3334	0.10953776966776281	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	3329	0.10937349586802113	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	3327	0.10930778634812444	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3235	0.10628514843287723	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3064	0.10066698448171123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2854759948339176E-5	0.0
3	0.0	0.0	0.0	6.570951989667835E-5	0.0
4	0.0	0.0	0.0	9.856427984501752E-5	0.0
5	0.0	0.0	0.0	1.314190397933567E-4	0.0
6	3.2854759948339176E-5	0.0	0.0	1.314190397933567E-4	0.0
7	3.2854759948339176E-5	0.0	0.0	1.314190397933567E-4	0.0
8	3.2854759948339176E-5	0.0	0.0	1.314190397933567E-4	0.0
9	3.2854759948339176E-5	0.0	0.0	2.628380795867134E-4	0.0
10	3.2854759948339176E-5	0.0	0.0	3.6140235943173094E-4	0.0
11	6.570951989667835E-5	0.0	0.0	8.542237586568186E-4	0.0
12	6.570951989667835E-5	0.0	0.0	0.0012156261180885494	0.0
13	6.570951989667835E-5	0.0	0.0	0.0014127546777785846	0.0
14	6.570951989667835E-5	0.0	0.0	0.001675592757365298	0.0
15	6.570951989667835E-5	0.0	0.0	0.002496961756073777	0.0
16	6.570951989667835E-5	0.0	0.0	0.0038440069139556833	0.0
17	6.570951989667835E-5	0.0	0.0	0.006012421070546069	0.0
18	6.570951989667835E-5	0.0	0.0	0.00676808054935787	0.0
19	6.570951989667835E-5	0.0	0.0	0.008115125707239776	0.0
20	6.570951989667835E-5	0.0	0.0	0.010480668423520197	0.0
21	6.570951989667835E-5	0.0	0.0	0.014653222936959273	0.0
22	6.570951989667835E-5	0.0	0.0	0.02349115336306251	0.0
23	6.570951989667835E-5	0.0	0.0	0.03643592878270815	0.0
24	6.570951989667835E-5	0.0	0.0	0.05529456099305483	0.0
25	6.570951989667835E-5	0.0	0.0	0.06344254146024295	0.0
26	6.570951989667835E-5	0.0	0.0	0.0742189027232982	0.0
27	6.570951989667835E-5	0.0	0.0	0.15819566915125313	0.0
28	6.570951989667835E-5	0.0	0.0	0.2382627191453557	0.0
29	6.570951989667835E-5	0.0	0.0	0.32946753276194524	0.0
30	6.570951989667835E-5	0.0	0.0	0.5144726860310431	0.0
31	6.570951989667835E-5	0.0	0.0	0.7362094609223843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10805	0.0	29.27811	1
GTATTGG	920	0.0	24.733696	1
ATTGGAC	950	0.0	22.978947	3
TTGGACC	1875	0.0	20.621334	4
ACGCATA	65	6.9069116E-5	19.923077	7
GGACCCT	1875	0.0	19.634668	6
TATTGGA	1215	0.0	18.576132	2
CTAGACG	50	0.007037482	18.5	4
ATCACGT	210	0.0	18.5	23
TGGACCC	2060	0.0	18.140778	5
GACCCTC	2035	0.0	17.545454	7
TTTTTAC	2410	0.0	17.195023	1
CGTGTCT	370	0.0	17.0	35
TATACTG	415	0.0	16.939758	5
CGTCTGT	1190	0.0	16.789915	34
GTATCAA	18875	0.0	16.770065	2
ACGTTTA	235	0.0	16.531916	26
CTAGCGG	80	3.384857E-4	16.1875	29
GTTATCA	415	0.0	16.048193	1
CGAACGA	70	0.0025938107	15.857142	16
>>END_MODULE