FastQCFastQC Report
Wed 25 May 2016
SRR2088202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360960
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT314461.3319158308484684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312181.3222587422065601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT276391.1706678639197614No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG148220.6277954730279208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111050.47035951477365134No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT81670.3459186093792356No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT79700.3375745459474112No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78830.3338896042287883No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA78120.33088235294117646No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67060.2840370018975332No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT64610.27365986717267554No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62080.26294388723231227No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG57070.24172370561127676No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51330.21741156139875303No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48120.20381539712659258No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47170.19979161019246408No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41590.17615715641095148No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.17014265383572785No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37560.15908782867985902No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35960.1523109243697479No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31400.13299674708593115No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.12948122797506098No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC29570.12524566278124152No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28410.12033240715641094No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG27990.11855346977500678No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.11478381675250746No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26750.11330136893467065No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC24760.10487259419896991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.029.5353241
ATTGGAC7600.020.2039473
TGCGACG656.906072E-519.92307722
GTATTGG7600.019.7171061
CGAATTA1653.6379788E-1217.93939415
CACGGTA650.001580601317.07692332
TAGTGCG650.001580601317.0769237
CGTCTGT6850.017.01459934
TTGGACC12650.016.6719364
CCGAATT1901.8189894E-1216.55263114
GTATCAA123050.016.4778562
ACGTTTA1352.2226232E-816.44444526
ACGGACC1251.6597733E-716.288
CAATGCG803.3844556E-416.187519
TGGACCC12450.015.8995985
TATACTG2800.015.8571435
GACCCTC12400.015.6653227
TACCCCG1302.5921508E-715.6538465
GGACCCT12750.015.5254916
TATACTC3500.014.85
Produced by FastQC (version 0.11.5)