##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2360960
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085391959203037	31.0	31.0	33.0	30.0	34.0
2	31.233006488885877	31.0	31.0	34.0	28.0	34.0
3	31.283790915559774	31.0	31.0	34.0	28.0	34.0
4	35.24689660138249	37.0	35.0	37.0	33.0	37.0
5	35.10938601246951	35.0	35.0	37.0	32.0	37.0
6	35.15993028259691	37.0	35.0	37.0	32.0	37.0
7	34.99605541813499	37.0	35.0	37.0	32.0	37.0
8	35.05274379913256	37.0	35.0	37.0	32.0	37.0
9	36.53783969232855	39.0	35.0	39.0	32.0	39.0
10	36.39813677487124	38.0	35.0	39.0	32.0	39.0
11	36.42714404310111	38.0	35.0	39.0	32.0	39.0
12	36.25959482583356	38.0	35.0	39.0	32.0	39.0
13	36.30993578883166	38.0	35.0	39.0	32.0	39.0
14	37.21638274261318	39.0	36.0	41.0	32.0	41.0
15	37.27588184467335	39.0	36.0	41.0	32.0	41.0
16	37.25556637977772	39.0	36.0	41.0	32.0	41.0
17	37.3517001558688	39.0	36.0	41.0	32.0	41.0
18	37.370370950799675	39.0	36.0	41.0	32.0	41.0
19	37.47089785510978	39.0	36.0	41.0	32.0	41.0
20	37.43052402412578	39.0	36.0	41.0	32.0	41.0
21	37.298181671862295	39.0	36.0	41.0	31.0	41.0
22	37.2550589590675	39.0	36.0	41.0	31.0	41.0
23	37.13095266332339	39.0	36.0	40.0	31.0	41.0
24	37.05166500067769	39.0	36.0	40.0	31.0	41.0
25	36.96731880252101	39.0	35.0	40.0	31.0	41.0
26	36.70030750203307	38.0	35.0	40.0	30.0	41.0
27	36.532002236378425	38.0	35.0	40.0	30.0	41.0
28	36.371150294795335	38.0	35.0	40.0	30.0	41.0
29	36.22770864394145	38.0	35.0	40.0	30.0	41.0
30	36.03725137232312	38.0	35.0	40.0	30.0	41.0
31	35.76908884521551	38.0	34.0	40.0	29.0	41.0
32	35.46604643873678	38.0	34.0	40.0	27.0	41.0
33	35.60696411629168	38.0	34.0	40.0	28.0	41.0
34	35.574351111412305	38.0	34.0	40.0	28.0	41.0
35	35.48276124966115	38.0	34.0	40.0	27.0	41.0
36	35.37875567565736	38.0	34.0	40.0	27.0	41.0
37	35.23669270127406	38.0	34.0	40.0	26.0	41.0
38	35.09958576172404	38.0	34.0	40.0	25.0	41.0
39	35.01118358633776	38.0	34.0	40.0	25.0	41.0
40	34.840587727026296	38.0	33.0	40.0	24.0	41.0
41	34.68546735226349	38.0	33.0	40.0	23.0	41.0
42	34.45569217606398	38.0	33.0	40.0	22.0	41.0
43	34.133939583898076	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	11.0
16	19.0
17	65.0
18	174.0
19	412.0
20	865.0
21	1737.0
22	3166.0
23	5608.0
24	9264.0
25	14594.0
26	22315.0
27	32083.0
28	44533.0
29	59578.0
30	76982.0
31	93944.0
32	114329.0
33	138997.0
34	167940.0
35	201669.0
36	250797.0
37	327614.0
38	397868.0
39	396391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76193582271618	22.690431011114125	14.152505760368664	22.39512740580103
2	17.523380319869883	23.025972485768502	36.526074139333154	22.924573055028464
3	19.810077256709135	26.135427961507183	32.35781207644348	21.6966827053402
4	13.084762130658714	15.672099484955273	35.355406275413394	35.88773210897262
5	11.03559568988886	40.83309331797235	34.62629608294931	13.505014909189484
6	31.468470452697204	36.07803605313093	15.526141908376253	16.92735158579561
7	24.288128557874764	35.59903598536189	21.465547912713472	18.647287544049878
8	27.711693548387096	33.47125745459474	20.296574274871237	18.520474722146922
9	25.374339251829763	13.509081051775548	24.07402073732719	37.04255895906749
10	16.340217538628355	27.81317769043101	33.69701307942532	22.149591691515315
11	31.24580679045812	21.868689007861207	25.269509013282732	21.61599518839794
12	20.228212252642994	27.720164678774733	31.695496747085933	20.35612632149634
13	31.045337489834644	21.1608413526701	28.846443819463268	18.947377338031988
14	20.921870764434804	22.584414814312822	28.879862428842507	27.613851992409867
15	22.832068311195446	29.157080170777988	27.305714624559503	20.705136893467063
16	21.695539102737868	28.302978449444293	27.456204255895905	22.54527819192193
17	19.11298793711033	27.20999085117918	28.339954933586338	25.337066278124155
18	20.126304554079695	26.62281444835999	32.90085388994307	20.35002710761724
19	19.512147600975872	24.942608091623747	34.373305773922475	21.171938533477906
20	24.005785782054758	23.965674979669288	32.849476484142045	19.17906275413391
21	23.408105177554894	24.856244917321767	31.57842572512876	20.15722417999458
22	21.642213336947684	24.539424640824073	32.419397194361615	21.39896482786663
23	20.60763418270534	26.5601704391434	33.52267721604771	19.30951816210355
24	19.31117003252914	25.66828747628083	31.910282258064516	23.11026023312551
25	20.358582949308758	26.644881743019788	33.196115139604224	19.800420168067227
26	21.90791034155598	25.524405326646786	31.681392314990514	20.886292016806724
27	20.4312652480347	27.045650921658986	31.29786188669016	21.225221943616155
28	20.174039373814043	26.519212523719165	31.56063635131472	21.74611175115207
29	19.32603686635945	26.42768195988073	32.411349620493354	21.83493155326647
30	18.638901124966115	26.6966827053402	34.44459033613445	20.21982583355923
31	20.165568243426403	26.060500813228515	33.269602195717	20.504328747628083
32	21.159570683111955	26.36990885063703	31.178037747357006	21.29248271889401
33	22.07047133369477	26.175581119544596	30.797811059907833	20.956136486852806
34	20.362776158850636	28.833398278666305	30.804842098129576	19.998983464353483
35	18.948605651938195	29.74146110056926	30.967996069395497	20.341937178097044
36	19.934136961236106	26.12687211981567	33.211786730821366	20.727204188126862
37	19.965564854974247	26.340852873407428	32.26882285172133	21.42475941989699
38	20.38073495527243	25.956136486852806	32.88827426131743	20.774854296557333
39	21.225857278395228	25.395982989970182	32.808137367850364	20.570022363784226
40	19.425064380590946	26.175665830848466	32.091733870967744	22.307535917592844
41	20.28793372187585	25.714455136893466	31.861785036595286	22.135826104635402
42	17.920337489834644	27.337184873949578	32.26166474654378	22.480812889672
43	18.544363309840065	27.090547912713475	31.999229127134726	22.365859650311737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	200.0
1	317.5
2	435.0
3	1226.5
4	2018.0
5	2018.0
6	3429.0
7	4840.0
8	5279.5
9	5719.0
10	8727.0
11	11735.0
12	11735.0
13	20661.0
14	29587.0
15	47912.0
16	66237.0
17	64941.0
18	63645.0
19	63645.0
20	66331.0
21	69017.0
22	55485.5
23	41954.0
24	45135.0
25	48316.0
26	48316.0
27	52549.0
28	56782.0
29	59323.0
30	61864.0
31	66476.0
32	71088.0
33	71088.0
34	78194.0
35	85300.0
36	94711.5
37	104123.0
38	107472.5
39	110822.0
40	110822.0
41	117249.5
42	123677.0
43	141462.5
44	159248.0
45	200148.0
46	241048.0
47	241048.0
48	243496.5
49	245945.0
50	220773.5
51	195602.0
52	180285.0
53	164968.0
54	164968.0
55	143901.0
56	122834.0
57	100006.5
58	77179.0
59	67069.0
60	56959.0
61	56959.0
62	50694.5
63	44430.0
64	39021.0
65	33612.0
66	28227.0
67	22842.0
68	22842.0
69	19112.5
70	15383.0
71	12620.5
72	9858.0
73	7826.5
74	5795.0
75	5795.0
76	4640.5
77	3486.0
78	2741.0
79	1996.0
80	1570.0
81	1144.0
82	1144.0
83	856.5
84	569.0
85	454.0
86	339.0
87	279.0
88	219.0
89	219.0
90	154.5
91	90.0
92	65.5
93	41.0
94	25.5
95	10.0
96	10.0
97	6.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2360960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.18311264182222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.77753676513701	28.516504208627218
2	13.563401359342183	10.629125665386988
3	5.318301516931379	6.251628222032886
4	2.5964614522222744	4.069497662102988
5	1.4532773442676028	2.847196494012287
6	0.8677926592485249	2.0401690510248844
7	0.5868056791542142	1.6095011117612399
8	0.4341931020128796	1.3610429779578292
9	0.3023726028566858	1.0663109781781044
>10	1.7973981411073918	13.59693874428305
>50	0.18584435082546735	5.0476098805353
>100	0.09939795820990116	7.362175261995733
>500	0.008063690396581216	2.135636722257462
>1k	0.007627815240009259	5.854705165993552
>5k	9.807191022869048E-4	2.649243306527825
>10k+	5.44843945714947E-4	4.962714547322661
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31446	1.3319158308484684	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31218	1.3222587422065601	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27639	1.1706678639197614	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14822	0.6277954730279208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11105	0.47035951477365134	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8167	0.3459186093792356	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7970	0.3375745459474112	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7883	0.3338896042287883	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7812	0.33088235294117646	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6706	0.2840370018975332	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6461	0.27365986717267554	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6208	0.26294388723231227	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5707	0.24172370561127676	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5133	0.21741156139875303	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4812	0.20381539712659258	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4717	0.19979161019246408	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4159	0.17615715641095148	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4017	0.17014265383572785	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3756	0.15908782867985902	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3596	0.1523109243697479	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3140	0.13299674708593115	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3057	0.12948122797506098	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2957	0.12524566278124152	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2841	0.12033240715641094	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2799	0.11855346977500678	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2710	0.11478381675250746	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2675	0.11330136893467065	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2476	0.10487259419896991	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.2355651938194635E-5	0.0
5	0.0	0.0	0.0	8.471130387638927E-5	0.0
6	4.2355651938194635E-5	0.0	0.0	8.471130387638927E-5	0.0
7	4.2355651938194635E-5	0.0	0.0	8.471130387638927E-5	0.0
8	4.2355651938194635E-5	0.0	0.0	8.471130387638927E-5	0.0
9	4.2355651938194635E-5	0.0	0.0	1.6942260775277854E-4	0.0
10	4.2355651938194635E-5	0.0	0.0	2.964895635673624E-4	0.0
11	4.2355651938194635E-5	0.0	0.0	5.929791271347248E-4	0.0
12	4.2355651938194635E-5	0.0	0.0	9.31824342640282E-4	0.0
13	4.2355651938194635E-5	0.0	0.0	0.001143602602331255	0.0
14	4.2355651938194635E-5	0.0	0.0	0.0015671591217132014	0.0
15	4.2355651938194635E-5	0.0	0.0	0.0024989834643534834	0.0
16	4.2355651938194635E-5	0.0	0.0	0.003091962591488208	0.0
17	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.004362632149634047	0.0
18	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.005040322580645161	0.0
19	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.005929791271347249	0.0
20	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.007581661696936839	0.0
21	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.010250067769043102	0.0
22	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.01728110599078341	0.0
23	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.02770059636757929	0.0
24	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.04519348061805367	0.0
25	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.052351585795608564	0.0
26	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.06217809704526972	0.0
27	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.14396686093792355	0.0
28	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.2296099891569531	0.0
29	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.3330001355380862	0.0
30	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.5319869883437246	0.0
31	4.2355651938194635E-5	4.2355651938194635E-5	0.0	0.8214878693412849	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6865	0.0	29.535324	1
ATTGGAC	760	0.0	20.203947	3
TGCGACG	65	6.906072E-5	19.923077	22
GTATTGG	760	0.0	19.717106	1
CGAATTA	165	3.6379788E-12	17.939394	15
CACGGTA	65	0.0015806013	17.076923	32
TAGTGCG	65	0.0015806013	17.076923	7
CGTCTGT	685	0.0	17.014599	34
TTGGACC	1265	0.0	16.671936	4
CCGAATT	190	1.8189894E-12	16.552631	14
GTATCAA	12305	0.0	16.477856	2
ACGTTTA	135	2.2226232E-8	16.444445	26
ACGGACC	125	1.6597733E-7	16.28	8
CAATGCG	80	3.3844556E-4	16.1875	19
TGGACCC	1245	0.0	15.899598	5
TATACTG	280	0.0	15.857143	5
GACCCTC	1240	0.0	15.665322	7
TACCCCG	130	2.5921508E-7	15.653846	5
GGACCCT	1275	0.0	15.525491	6
TATACTC	350	0.0	14.8	5
>>END_MODULE