FastQCFastQC Report
Wed 25 May 2016
SRR2088201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607101
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT232420.8914882852639772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT222670.8540904245750356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204760.7853934312479647No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG123380.4732459540309332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84080.32250380786935373No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70350.2698399486632854No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA66400.25468902048673986No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT61790.23700654481740446No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT59030.2264200734839195No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA48530.18614545428044407No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT47110.18069879149292645No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG46220.17728503805567947No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT41650.15975598950711922No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39530.15162435210603653No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37430.14356942826534147No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34530.1324459620091435No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28870.11073602441946054No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC27980.10732227098221357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27520.10555785909329941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55950.028.0393221
ATTGGAC10550.019.1137433
CAATGCG1002.8784052E-718.49999819
GTATTGG11050.017.4117641
CGACGAG1301.3953468E-817.07692324
TTGGACC15850.016.5741334
TGGACCC14950.016.0869565
GTATCAA98200.016.0320782
GGACCCT14900.015.8926176
ACCGTTA1403.4784534E-815.8571438
GAACCGT957.065166E-515.5789486
TATTGGA14400.014.9027782
GTCCGAC750.004106261314.80000130
TAGCGAG1001.09420915E-414.79999928
ACCGTCC1152.21186E-514.4782618
GACCCTC16500.014.0151527
CCGGTAG1601.7859384E-713.8759
CGGTGTC1501.3072022E-613.56666830
CCGTTTA1501.3072022E-613.56666827
TGCGACG1254.84899E-513.3222
Produced by FastQC (version 0.11.5)