##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2607101
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14675419172483	31.0	31.0	34.0	30.0	34.0
2	31.30481366084398	31.0	31.0	34.0	30.0	34.0
3	31.368624383942166	31.0	31.0	34.0	28.0	34.0
4	35.30956031239296	37.0	35.0	37.0	33.0	37.0
5	35.16991670058045	37.0	35.0	37.0	32.0	37.0
6	35.210475927092965	37.0	35.0	37.0	32.0	37.0
7	35.058576940440744	37.0	35.0	37.0	32.0	37.0
8	35.10661497195544	37.0	35.0	37.0	32.0	37.0
9	36.64397351694468	39.0	35.0	39.0	32.0	39.0
10	36.478046688639985	38.0	35.0	39.0	32.0	39.0
11	36.49950692359061	38.0	35.0	39.0	32.0	39.0
12	36.34063390716355	38.0	35.0	39.0	32.0	39.0
13	36.39497088912167	38.0	35.0	39.0	32.0	39.0
14	37.36342473881909	39.0	36.0	41.0	32.0	41.0
15	37.426139608707146	39.0	36.0	41.0	32.0	41.0
16	37.404776416410414	39.0	36.0	41.0	32.0	41.0
17	37.45633176466888	39.0	36.0	41.0	32.0	41.0
18	37.45458538046665	39.0	36.0	41.0	32.0	41.0
19	37.53360571761508	39.0	36.0	41.0	32.0	41.0
20	37.49660945241477	39.0	36.0	41.0	32.0	41.0
21	37.36832328321764	39.0	36.0	41.0	31.0	41.0
22	37.31804099649381	39.0	36.0	41.0	31.0	41.0
23	37.21805330902025	39.0	36.0	41.0	31.0	41.0
24	37.1240458271467	39.0	36.0	41.0	31.0	41.0
25	37.048675137633715	39.0	36.0	40.0	31.0	41.0
26	36.8099252771565	39.0	35.0	40.0	30.0	41.0
27	36.66035914987567	39.0	35.0	40.0	30.0	41.0
28	36.53230120352069	38.0	35.0	40.0	30.0	41.0
29	36.40646296403553	38.0	35.0	40.0	30.0	41.0
30	36.250185550924186	38.0	35.0	40.0	30.0	41.0
31	36.04693680835533	38.0	35.0	40.0	29.0	41.0
32	35.75882292247213	38.0	34.0	40.0	28.0	41.0
33	35.963185929505606	38.0	35.0	40.0	29.0	41.0
34	35.9777921914034	38.0	35.0	40.0	29.0	41.0
35	35.907964056628416	38.0	35.0	40.0	29.0	41.0
36	35.842397743700765	38.0	35.0	40.0	29.0	41.0
37	35.7338384665573	38.0	35.0	40.0	28.0	41.0
38	35.63259344382899	38.0	34.0	40.0	27.0	41.0
39	35.632967038868074	38.0	34.0	40.0	27.0	41.0
40	35.52394786393009	38.0	34.0	40.0	27.0	41.0
41	35.42610125192695	38.0	34.0	40.0	26.0	41.0
42	35.24972488599406	38.0	34.0	40.0	25.0	41.0
43	34.968501412104864	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	7.0
15	17.0
16	25.0
17	90.0
18	184.0
19	426.0
20	856.0
21	1669.0
22	3094.0
23	5231.0
24	8831.0
25	13801.0
26	21268.0
27	31142.0
28	43600.0
29	59493.0
30	76641.0
31	95821.0
32	118193.0
33	144969.0
34	178469.0
35	217775.0
36	272573.0
37	356629.0
38	470157.0
39	486138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67126666745937	22.637289464428115	13.476961575328305	23.214482292784208
2	17.22299979939404	23.114256026137845	36.97750873479777	22.685235439670347
3	19.352453165412463	26.65355120495907	31.95982817696744	22.03416745266102
4	12.814770122062782	16.29664520093391	37.16653094759275	33.72205372941056
5	11.596482069547747	39.93723296489089	35.4495663957783	13.016718569783064
6	31.01184035447802	37.59305834334765	16.12377119260052	15.271330109573814
7	24.87659664892154	34.41051957710883	22.655355507899387	18.057528266070243
8	26.38996341146737	34.77441035080728	20.861792466037947	17.973833771687403
9	25.29284442758451	13.983271073886282	23.254756911987684	37.46912758654153
10	15.74369385766029	27.441552897260213	34.87375441150918	21.940998833570312
11	31.95046912260016	22.431045057326127	24.953271852528918	20.665213967544794
12	19.661378673093218	27.203549076157767	32.35037691290057	20.78469533784844
13	29.880583836222684	21.15802187947456	28.499432895004833	20.461961389297922
14	20.634106618807632	22.757154402533693	28.339561835157134	28.26917714350154
15	22.79305634879508	29.461459299045185	26.244591214532925	21.50089313762681
16	21.80299113843307	28.933401506117328	26.95008747263723	22.313519882812365
17	19.720141260350097	27.618799578535697	28.23569167439236	24.425367486721843
18	20.810317667017884	26.93700781059115	31.411441290536885	20.84123323185408
19	20.219546538473192	25.897308926658386	32.272474292327	21.610670242541428
20	23.427477493200303	25.23599967933732	31.217355982756327	20.119166844706054
21	23.246932128828153	25.776216571586602	30.280146415501356	20.696704884083893
22	21.875677236900295	25.49126405152696	31.095841703102412	21.53721700847033
23	20.965624270022527	26.74986508002567	31.92235360271812	20.36215704723369
24	20.25575533897613	26.29936469664965	30.668278674282277	22.776601290091943
25	21.19208270028664	26.895122206619536	31.259088159607167	20.653706933486657
26	22.030446844982222	26.350992922790482	30.349418760531332	21.269141471695956
27	21.156257467585643	27.33657038986982	30.098488704503584	21.408683438040953
28	20.981580690583144	26.802797436693094	30.313248316808593	21.902373555915172
29	20.24025919977784	26.872645133425976	31.058290415292696	21.82880525150349
30	19.795704117331855	27.123153264871597	32.21064316265461	20.87049945514194
31	20.926922278806998	26.659266365207944	31.439710237539703	20.974101118445354
32	21.3688691001998	26.63318375467617	30.114713622525557	21.883233522598474
33	22.061669264059965	26.538864432179647	30.001561120953884	21.397905182806497
34	21.05883124589343	28.35897036593519	29.874868675973808	20.707329712197573
35	20.251497736374617	29.138802064055056	30.121502772619856	20.48819742695047
36	20.726047820932138	26.70905346589948	31.377303756164416	21.187594957003967
37	20.916987872736804	26.59498040160316	30.861366705777797	21.62666501988224
38	20.9614817377616	26.24202131025994	31.48688907717806	21.309607874800403
39	21.52333185404018	25.86221247278107	31.526434917557854	21.0880207556209
40	20.397560355352553	26.40181565654725	31.056756144084947	22.14386784401525
41	20.989021905940735	26.009349081604434	30.920704644737583	22.080924367717248
42	19.36599310882087	27.336071751727303	31.204583174951793	22.093351964500034
43	19.68723114294383	27.072752455696957	30.856610465033768	22.383405936325442
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	216.5
2	324.0
3	986.0
4	1648.0
5	1648.0
6	2869.0
7	4090.0
8	4362.5
9	4635.0
10	7044.5
11	9454.0
12	9454.0
13	16514.5
14	23575.0
15	36803.5
16	50032.0
17	50199.5
18	50367.0
19	50367.0
20	55463.0
21	60559.0
22	53426.0
23	46293.0
24	52420.5
25	58548.0
26	58548.0
27	65566.0
28	72584.0
29	78942.0
30	85300.0
31	92774.5
32	100249.0
33	100249.0
34	107852.0
35	115455.0
36	125532.0
37	135609.0
38	141418.5
39	147228.0
40	147228.0
41	150660.0
42	154092.0
43	164468.0
44	174844.0
45	203667.5
46	232491.0
47	232491.0
48	233821.0
49	235151.0
50	216749.0
51	198347.0
52	187397.5
53	176448.0
54	176448.0
55	153473.0
56	130498.0
57	111249.5
58	92001.0
59	81005.5
60	70010.0
61	70010.0
62	61151.0
63	52292.0
64	45494.5
65	38697.0
66	32866.0
67	27035.0
68	27035.0
69	23398.0
70	19761.0
71	17040.5
72	14320.0
73	11984.0
74	9648.0
75	9648.0
76	7537.0
77	5426.0
78	4470.5
79	3515.0
80	2886.0
81	2257.0
82	2257.0
83	2004.5
84	1752.0
85	1428.5
86	1105.0
87	920.5
88	736.0
89	736.0
90	619.0
91	502.0
92	288.5
93	75.0
94	51.0
95	27.0
96	27.0
97	16.5
98	6.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2607101.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.57026299284425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45313758890657	36.64778736328454
2	13.724473882051866	13.332026117793625
3	4.86003308420085	7.081592551606778
4	2.116176099020826	4.11132918674451
5	1.0710769824217679	2.6011245360903636
6	0.6197464621659863	1.8060749193772048
7	0.39653167563009584	1.3481753439242876
8	0.2605738704196541	1.0124913132276734
9	0.1840997040988537	0.8047593940487519
>10	1.1095697206455633	10.28581720523033
>50	0.11683564472916358	3.932334912702648
>100	0.07837471804468624	7.307523416573411
>500	0.004447049060934778	1.4888567164651858
>1k	0.00420881428981253	3.9013978101715896
>5k	3.9705795186910657E-4	1.317758340924492
>10k+	3.1764636149528525E-4	3.0209508718345965
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23242	0.8914882852639772	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22267	0.8540904245750356	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20476	0.7853934312479647	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12338	0.4732459540309332	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8408	0.32250380786935373	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7035	0.2698399486632854	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6640	0.25468902048673986	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6179	0.23700654481740446	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5903	0.2264200734839195	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4853	0.18614545428044407	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4711	0.18069879149292645	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4622	0.17728503805567947	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4165	0.15975598950711922	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3953	0.15162435210603653	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3743	0.14356942826534147	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3453	0.1324459620091435	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2887	0.11073602441946054	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2798	0.10732227098221357	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2752	0.10555785909329941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.8356780193786126E-5	0.0
5	7.671356038757225E-5	0.0	0.0	3.8356780193786126E-5	0.0
6	7.671356038757225E-5	0.0	0.0	3.8356780193786126E-5	0.0
7	7.671356038757225E-5	0.0	0.0	3.8356780193786126E-5	0.0
8	1.1507034058135837E-4	0.0	0.0	3.8356780193786126E-5	0.0
9	1.1507034058135837E-4	0.0	0.0	1.1507034058135837E-4	0.0
10	1.1507034058135837E-4	0.0	0.0	2.684974613565029E-4	0.0
11	1.1507034058135837E-4	0.0	0.0	5.753517029067919E-4	0.0
12	1.534271207751445E-4	0.0	0.0	0.0011507034058135838	0.0
13	1.534271207751445E-4	0.0	0.0	0.0011507034058135838	0.0
14	1.534271207751445E-4	0.0	0.0	0.0013424873067825144	0.0
15	1.534271207751445E-4	0.0	0.0	0.002071266130464451	0.0
16	1.534271207751445E-4	0.0	0.0	0.003413753437246965	0.0
17	1.534271207751445E-4	0.0	0.0	0.004871311084610838	0.0
18	1.534271207751445E-4	0.0	0.0	0.00544666278751763	0.0
19	1.534271207751445E-4	0.0	0.0	0.005906944149843063	0.0
20	1.534271207751445E-4	0.0	0.0	0.007632999258563439	0.0
21	1.534271207751445E-4	0.0	0.0	0.010816612014647687	0.0
22	1.534271207751445E-4	0.0	0.0	0.019868812140381213	0.0
23	1.534271207751445E-4	0.0	0.0	0.034252604713051006	0.0
24	1.534271207751445E-4	0.0	0.0	0.05266385920606835	0.0
25	1.534271207751445E-4	0.0	0.0	0.05868587369649277	0.0
26	1.534271207751445E-4	0.0	0.0	0.0666257271966065	0.0
27	1.534271207751445E-4	0.0	0.0	0.12366225934476646	0.0
28	1.534271207751445E-4	0.0	0.0	0.1838056906886231	0.0
29	1.534271207751445E-4	0.0	0.0	0.25676028661720435	0.0
30	1.534271207751445E-4	0.0	0.0	0.3890144647253789	0.0
31	1.9178390096893062E-4	0.0	0.0	0.6085303177744169	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5595	0.0	28.039322	1
ATTGGAC	1055	0.0	19.113743	3
CAATGCG	100	2.8784052E-7	18.499998	19
GTATTGG	1105	0.0	17.411764	1
CGACGAG	130	1.3953468E-8	17.076923	24
TTGGACC	1585	0.0	16.574133	4
TGGACCC	1495	0.0	16.086956	5
GTATCAA	9820	0.0	16.032078	2
GGACCCT	1490	0.0	15.892617	6
ACCGTTA	140	3.4784534E-8	15.857143	8
GAACCGT	95	7.065166E-5	15.578948	6
TATTGGA	1440	0.0	14.902778	2
GTCCGAC	75	0.0041062613	14.800001	30
TAGCGAG	100	1.09420915E-4	14.799999	28
ACCGTCC	115	2.21186E-5	14.478261	8
GACCCTC	1650	0.0	14.015152	7
CCGGTAG	160	1.7859384E-7	13.875	9
CGGTGTC	150	1.3072022E-6	13.566668	30
CCGTTTA	150	1.3072022E-6	13.566668	27
TGCGACG	125	4.84899E-5	13.32	22
>>END_MODULE