FastQCFastQC Report
Wed 25 May 2016
SRR2088200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866447
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT315201.099619145234501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310051.081652652220676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT278220.9706092594769763No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG170740.5956502945981559No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113300.39526284630415287No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92640.32318755588364273No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA91260.3183732334838216No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT87010.30354651594814064No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT86180.30065094522940766No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA69930.24396055465180413No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT65800.22955247384654243No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG64760.22592428884957583No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT63780.2225054222178188No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54060.18859584705386145No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA50880.17750197369775195No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50130.1748854941326318No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT44960.15684922833040346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.13406841291675722No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38170.13316136666751557No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35550.1240211313866958No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34610.12074181033174519No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31970.11153180226252221No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT31520.10996191452345011No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30140.10514759212362901No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29990.10462429621060497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.029.724721
GTATTGG11550.020.0216451
ACGTTTA1501.2732926E-1118.526
GGACCCT17350.017.593666
CGCACTA752.0686245E-417.26666619
CGAACTA2150.017.20930324
ATTGGAC13050.017.1532573
CGTCTGT7900.017.09493634
TGGACCC18350.016.937335
GTATCAA126500.016.7889352
TTGGACC19300.016.1994824
GACCCTC18750.015.5893337
GTACCGT1101.453295E-515.1363646
CACGGTA750.004106395414.832
ACGGACC1551.2120472E-714.322588
TATTGGA15650.014.3035132
TATATCG800.006301650313.8755
TGCGTCT8650.013.687860532
TCACGTT1907.1395334E-913.63157924
AAGACCG1409.569923E-613.2142865
Produced by FastQC (version 0.11.5)